Sage Journals: Discover world-class research

Abstract

Membrane contact sites (MCS) circumvent the topological constraints of functional coupling between different membrane-bound organelles by providing a means of communication and exchange of materials. One of the most characterised contact sites in the cell is that between the endoplasmic reticulum and the mitochondrial (ERMCS) whose function is to couple cellular Ca²⁺ homeostasis and mitochondrial function. Inositol 1,4,5-trisphosphate receptors (IP₃Rs) on the ER, glucose-regulated protein 75 (GRP 75) and voltage-dependent anion channel 1 (VDAC1) on the outer mitochondrial membrane are the canonical component of the Ca²⁺ transfer unit at ERMCS. These are often reported to form a Ca²⁺ funnel that fuels the mitochondrial low-affinity Ca²⁺ uptake system. We assess the available evidence on the IP₃R subtype selectivity at the ERMCS and consider if IP₃Rs have other roles at the ERMCS beyond providing Ca²⁺. Growing evidence suggests that all three IP₃R subtypes can localise and regulate Ca²⁺ signalling at ERMCS. Furthermore, IP₃Rs may be structurally important for assembly of the ERMCS in addition to their role in providing Ca²⁺ at these sites. Evidence that various binding partners regulate the assembly and Ca²⁺ transfer at ERMCS populated by IP₃R-GRP75-VDAC1, suggesting that cells have evolved mechanisms that stabilise these junctions forming a Ca²⁺ microdomain that is required to fuel mitochondrial Ca²⁺ uptake.

Keywords

endoplasmic reticulum mitochondria membrane contact sites VDAC1 GRP75

Highlight

IP₃Rs may have a structural role in forming ERMCS

All IP₃R subtypes localise to ER–mitochondrial contact sites

Various tethering complexes and binding partners ensure the stability of ERMCS where IP₃R delivers Ca²⁺ to the mitochondria

Introduction

Membrane contact sites (MCS) provide a means of biochemical exchange of signalling mediators between distinct membran e-bound organelles. For cellular homeostasis, intracellular compartments are required to cooperate via functional junctions in order to regulate various processes including Ca²⁺ signalling, lipid synthesis and trafficking (Chang and Liou, 2016, Zaman et al., 2020), glucose homeostasis (Rieusset, 2018), mitochondrial nucleoid transportation (Qin et al., 2020), autophagy (Kohler et al., 2020), organelle dynamics (Prinz, 2014) and various pathological processes (Kim et al., 2022). MCS are characterised as the regions of proximity between two organelles or cellular compartments that do not completely fuse but are held apart at short distances of ∼30 nm by distinct tethering complexes (Fernandez-Busnadiego et al., 2015; Prinz, 2014). First reported over 60 years ago, the endoplasmic reticulum (ER)–mitochondrial junction is one of the most characterised MCS (Copeland and Dalton, 1959). Tethering complexes of various compositions have been identified between the mitochondria and the ER, posing an important need to understand if these signify the occurrence of functionally distinct junctions, or if independent tethering complexes contribute to the maintenance of the same junctions, so facilitating the overall functions of these contact sites.

The composition of the ER–mitochondrial MCS (ERMCS) has been thoroughly reviewed previously (de Brito and Scorrano, 2010, Prinz, 2014, Sassano et al., 2022, Xu et al., 2020). The morphology and composition of these junctions are not static but are dynamic and responsive to changing cellular metabolic demands. Cryogenic-electron microscopy (Cryo-EM) analysis has shown that the length of ERMCS changes in response to nutrient availability (Sood et al., 2014), while biochemical analysis reveals a change in the number of ERMCS during transitions in nutrient availability (Theurey et al., 2016). The onset of ER stress is reportedly marked by an increase in ERMCS and subsequent changes in mitochondrial functions that drive the cellular adaptation to this signal (Bravo et al., 2011). Similarly, a change in ER–mitochondria coupling is also observed following chemically-induced mitochondrial stress (Lopez-Crisosto et al., 2021). Advanced imaging techniques have also shown the dynamism of these junctions, revealing highly motile VAMP-associated protein B (VAPB) positive contact sites that are altered in response to nutrient stress (Obara et al., 2022). In this review, we will discuss the role of ERMCS in shaping the delivery of Ca²⁺ to the mitochondria. Specifically, we will highlight the role and regulation of component proteins that drive the stability and delivery of Ca²⁺ at these junctions.

ERMCS and Ca²⁺ Signalling

The role of ERMCS in shaping Ca²⁺ signals is one of the most characterised functions of these contact sites. Ca²⁺ must permeate the outer mitochondrial membrane (OMM) and the inner mitochondrial membrane (IMM) to reach the mitochondrial matrix. The OMM has a high Ca²⁺ permeability. This is possibly due to its abundant expression of voltage-dependent anion-selective channel proteins (VDACs) (of which there are 3 subtypes (VDAC1-3)); although VDAC1 is predominantly implicated in mitochondrial Ca²⁺ dynamics, it was shown to selectively co-immunoprecipitate with inositol 1,4,5-trisphosphate receptors (IP₃R; see later)(De Stefani et al., 2012; Gincel et al., 2001, Rapizzi et al., 2002). There may yet be unidentified Ca²⁺ permeable channels on the OMM that drive its high permeability to Ca²⁺ as the loss of all VDAC isoforms does not affect mitochondrial-dependent cell death, a process thought to occur following mitochondrial Ca²⁺ overload (Baines et al., 2007). Following many years of debate, the mitochondrial Ca²⁺ uniporter (MCU) was identified as the mediator of Ca²⁺ transport through the IMM into the mitochondrial matrix (De Stefani et al., 2011; Kirichok et al., 2004). The Ca²⁺ uptake into mitochondria is now strongly encouraged to involve a tightly regulated multiprotein complex (reviewed in (Giorgi et al. 2018), (De Stefani et al., 2015)). The low affinity of the MCU (K_D Ca²⁺ for MCU, ∼20 −30 µM) is evolutionary important to avoid unregulated Ca²⁺ overload of the mitochondria for triggering apoptosis (Bragadin et al., 1979). The accumulation of high concentrations of Ca²⁺ in the mitochondria occurs following the stimulation of Ca²⁺ channels on the ER. This is only made possible due to their close apposition to the ER (Csordas et al., 2010; Rizzuto et al., 1998). Between 5% and 20% of the surface of the mitochondrial network in HeLa cells is in contact with the ER under resting conditions (Rizzuto et al., 1998). The canonical view of the ERMCS protein complex driving Ca²⁺ exchange is the interaction of IP₃R with VDAC1 via the cytosolic protein linker GRP75. GRP75 is part of the heat shock protein 70 family of proteins and is reported to stabilise the IP₃R-VDAC1 interaction and therefore promote the formation of ERMCS enabling Ca²⁺ transfer into the mitochondria (Xu et al., 2018). It is important to note that other than at ERMCS, mitochondrial Ca²⁺ uptake dynamics have also been implicated in regulating Ca²⁺ signals at ER–PM membrane contact sites by regulating STIM1 oligomerisation at these junctions (Deak et al., 2014, Nan et al., 2021).

The transfer of Ca²⁺ at ERMCS not only shapes the spatiotemporal properties of global Ca²⁺ signals but also directly affects mitochondria functions. For example, the activity of three of the important enzymes in the electron transport chain pyruvate dehydrogenase, isocitrate dehydrogenase, oxoglutarate dehydrogenase (OGDH) and α-ketoglutarate dehydrogenase is Ca²⁺-dependent (Denton, 2009). Ca²⁺ signalling at ERMCS is also an important regulator of cellular apoptosis (Kroemer and Reed, 2000). Ca²⁺ overload into the mitochondria is an important trigger for apoptosis leading to mitochondrial swelling, the opening of the mitochondrial permeability transition pore (mPTP) and release into the cytosol of apoptotic factors such as cytochrome c, procaspase 9 and apoptosis-inducing factor 9 (Giorgi et al. 2018, Sukumaran et al., 2021). Furthermore, B cell lymphoma 2 (Bcl-2), mainly resident on the OMM, is an anti-apoptotic protein which reduces the steady-state ER Ca²⁺ concentration and subsequent Ca²⁺ transfer to the mitochondria so enhancing the apoptotic signals (Pinton and Rizzuto, 2006, Sukumaran et al., 2021). Conversely, the pro-apoptotic protein BAX promotes apoptosis by increasing Ca²⁺ transfer to the mitochondria from the ER (Sukumaran et al., 2021). VDAC1 oligomerisation is also implicated in regulating apoptosis (Shoshan-Barmatz et al., 2018). Moreover, a reduction in mitochondrial Ca²⁺ uptake, such as following down-regulation of IP₃R on the ER, leads to the impairment of cellular bioenergetics and the activation of autophagy, a process where obsolete cellular components are degraded or recycled in lysosomes (Sukumaran et al., 2021). It is therefore not unexpected that disruptions of ERMCS are implicated in the pathophysiology of multiple diseases such as amyotrophic lateral sclerosis (Sakai et al., 2021), Alzheimer's disease (Area-Gomez et al., 2009, Yu et al., 2021), Parkinson's disease (Guardia-Laguarta et al., 2014), Huntington's disease (Kim et al., 2010; Milakovic et al., 2006) and cancer (Simoes et al., 2020) (Figure 1).

Figure 1.

Ca²⁺ Transfer at ER–Mitochondria Contact Sites Is Important for Cellular Homeostasis. IP₃R forms a tightly regulated complex with GRP75 in the cytosol and VDAC1 in the outer mitochondrial membrane to allow preferential access of high Ca²⁺ concentrations to fuel the low-affinity MCU. Ca²⁺ taken up into the mitochondria is important for mitochondrial function and dynamics. Dysregulation of ERMCS abrogates mitochondrial Ca²⁺ uptake with consequences for cellular Ca²⁺ dynamics and various pathophysiological processes.

Localisation and Functioning of IP₃Rs at ERMCS

IP₃Rs are high-conductance intracellular Ca²⁺ channels, ubiquitously expressed in eukaryotic cells, which open in response to extracellular stimuli. Ca²⁺ signals elicited by IP₃Rs regulate a vast plethora of biological processes such as motility, neurotransmitter release, apoptosis and gene transcription (Berridge, 2016; Clapham, 2007). IP₃Rs are predominantly located on the ER but have also been reported to be in the nuclear envelope, plasma membrane, Golgi and secretory vesicles (Dellis et al., 2006; Malviya et al., 1990; Pinton et al., 1998, Yoo and Albanesi, 1990). There are 3 subtypes of IP₃R, which have a molecular mass of ∼300 kDa and share 60-80% sequence homology, although they are encoded by different genes and expressed differentially in tissues (Foskett et al., 2007; Taylor et al., 1999). The existence of alternative splice variants confers further diversity to IP₃R expression in cells (Foskett et al., 2007).

Functionally, IP₃R exists as tetrameric channels which can be homomeric or heteromeric (Alzayady et al., 2013). IP₃R are co-regulated by binding to IP₃ and Ca²⁺, with the latter regulating the channel in a biphasic manner such that at low cytosolic concentrations of Ca²⁺ in the presence of IP₃ activates the channel (Mak and Foskett, 1998) while high Ca²⁺ concentrations conversely inhibit the IP₃ receptor (Kaftan et al., 1997, Mak and Foskett, 1998). Recent insights into the structure of IP₃Rs continue to improve our understanding of the structural basis for IP₃R activation and channel opening (Schmitz et al., 2022). Whilst most cells express more than one IP₃R subtype, preferential expression of the subtypes has been reported, with IP₃R1 reported as the predominant subtype in Purkinje cell neurons (Nakanishi et al., 1991), IP₃R2 in cardiac myocytes (Vervloessem et al., 2015) but also in the liver and epithelium (Klar et al., 2014), and IP₃R3 in pancreatic β cells (Blondel et al., 1994), testis and endothelial cells (De Smedt et al., 1997). While all three IP₃R subtypes are regulated by IP₃ and Ca²⁺, they have different IP₃ affinities in the order IP₃R2 > IP₃R1 > IP₃R3 (Iwai et al., 2007, Miyakawa et al., 1999; Newton et al., 1994; Tu et al. 2005b, Zhang et al., 2011) and are distinctly modulated by additional signals such as Ca²⁺ and ATP (Mak et al., 2001; Prole and Taylor, 2016, Tu et al. 2005a, Wagner and Yule, 2012; Yoneshima et al., 1997). In systems containing all three IP₃R subtypes, the composition of IP₃R channels that participate in Ca²⁺ release remains a question, as minor subtypes could be selectively contributing to the Ca²⁺ signals while the major subtype remains silent (Lock et al., 2018).

The Ca²⁺ signals evoked by IP₃R occur in a hierarchical manner, with increasing IP₃ stimulus corresponding to a graded response from local Ca²⁺ events, such as Ca²⁺ puffs involving the opening of a few IP₃R to global Ca²⁺ waves that spread across the entire cell (Berridge et al., 2000). All IP₃R subtypes can evoke Ca²⁺ puffs with a similar frequency and amplitude, but IP₃R2's high affinity for IP₃ is reflected in the slower kinetics of individual puffs (Lock et al., 2018, Mataragka and Taylor, 2018). The original notion that Ca²⁺ puffs are the building blocks of all modes of Ca²⁺ signalling (Berridge, 1997, Bootman and Berridge, 1996, Bootman et al., 1997, Marchant et al., 1999, Parker et al., 1996) has recently been challenged; it has been suggested that, sustained global signals are evoked by a diffuse mode of Ca²⁺ release distinct from Ca²⁺ puffs which terminate during the early stages of global Ca²⁺ waves (Lock and Parker, 2020). Another layer of complexity in the strict regulation of IP₃R activity is that not all IP₃Rs are licensed to release Ca²⁺. The majority of IP₃Rs in a cell are mobile. However, Ca²⁺ signals originate from a subset of IP₃R immobilised on actin via KRas-induced actin-binding protein (KRAP). The loss of KRAP reduces the number of immobile IP₃R clusters in a cell, abrogates Ca²⁺ puffs and global Ca²⁺ signals (Thillaiappan et al., 2021). There are thought to be approximately 8 IP₃Rs in a licensed IP₃R cluster from which elementary Ca²⁺ events occur (Thillaiappan et al., 2017, 2021). However, further analysis has suggested that KRAP may license individual IP₃R channels rather than the entire cluster as a signalling unit (Vorontsova et al., 2022). Furthermore, cyclic AMP response element-binding protein (CREB) may affect IP₃R1 licensing by regulating the expression of KRAP in HEK293 cells (Arige et al., 2021). Even with our increased knowledge of the structure and function of IP₃R, there continues to be active research on the fine-tuning of its licensing, activity and spatial localisation.

All IP₃R Subtypes Localise to ER–Mitochondrial Contact Sites

With different biophysical properties of the IP₃R subtypes, there has been a long interest in the subtype-specific regulation of ER–mitochondrial contact sites. Initial analysis into the importance of subtype selectively in the role of IP₃R at ER–mitochondria MCS in CHO-KI and HEK293 cells has suggested a preferentiality for IP₃R3 in delivering Ca²⁺ to mitochondria and subsequent regulation of apoptosis (Mendes et al., 2005). Furthermore, Mendes et al. using confocal microscopy suggested that IP₃R3 colocalises more extensively with mitochondria compared to the other 2 subtypes (Mendes et al., 2005). For many years it was thus considered that IP₃R3 selectively interacts with mitochondria at ERMCS and is the required mediator of apoptotic Ca²⁺ overload. Nonetheless, there have been isolated studies showing the roles of the other IP₃R subtypes at ERMCS in different biological settings. Recombinant expression of IP₃R1 was shown to enhance Ca²⁺ accumulation in mitochondria in the rat liver and HeLa cells (Szabadkai et al., 2006). Furthermore, IP₃R1 forms a tripartite complex with GRP75 and VDAC1 modulating diabetic atrial remodelling (Yuan et al., 2022). In astrocytes, IP₃R2 was found to be accumulated together with mitochondria at the sites of regenerative Ca²⁺ wave initiation (Simpson et al., 1998). However, in a systematic study, Bartok et al. showed that all IP₃R subtypes are competent to restore contacts between the ER and mitochondria in a null IP₃R background with compromised ERMCS (Bartok et al., 2019). Super-resolution analysis shows that all IP₃R subtypes can form clusters near to the mitochondria, but IP₃R2 may be more enriched at ER–mitochondrial junctions in DT40 cells. Whilst all IP₃R subtypes were found to be competent in transferring Ca²⁺ to mitochondria, IP₃R2 displayed higher efficacy in mediating Ca²⁺ transfer from the ER to the mitochondria in DT40 cells (Bartok et al., 2019). This work further suggests that IP₃R1-mediated Ca²⁺ transfer to the mitochondria may be predominantly sustained by Ca²⁺ entry across the plasma membrane in DT40 cells (Bartok et al., 2019). Therefore, there is growing evidence of the localisation of all IP₃R subtypes at ERMCS (Table 1). Further assessment of the context-specific contribution of IP₃R subtypes to ERMCS formation and function is required to address the potential involvement of all three subtypes in junction stability and Ca²⁺ transfer from the ER to the mitochondria.

Table 1.

Summary of IP₃R subtype identification at ERMCS in different cell types.

Subtype	Cell line	Comment	Reference
IP₃R1	DT40	IP₃R1 may increase [Ca²⁺]_mito, fuelled by Ca²⁺ influx from PM	(Bartok et al., 2019)
	Rat liver	IP₃R1 forms a complex with GRP75 and VDAC1	(Szabadkai et al., 2006)
	HeLa	IP₃R1 forms a complex with GRP75 and VDAC1	(Szabadkai et al., 2006)
IP₃R2	Astrocytes	IP₃R2 sits with mitochondria at the sites of regenerative Ca²⁺ signals	(Simpson et al., 1998)
IP₃R2	DT40	Suggests all IP₃R can exist at ERMCS but IP₃R2 most effective in Ca²⁺ transfer to mitochondria	(Bartok et al., 2019)
IP₃R3	HeLa	IP₃R3 forms a complex with GRP75 and VDAC1	(Gomez-Suaga et al., 2017)
	HEK	siRNA to IP₃R3 selectively reduces [Ca²⁺]_mito	(Mendes et al., 2005)
	CHO	siRNA to IP₃R3 selectively reduces [Ca²⁺]_mito	(Mendes et al., 2005)
	C57B6/J mice brain slices	IP₃R3 at MAM fractions with VDAC1, Tom70 and VAPB	(Filadi et al., 2018)

IP₃Rs Have a Structural Role in Forming ERMCS

Recent evidence suggests that IP₃Rs may play a structural role in ERMCS formation which is independent of their ability to deliver Ca²⁺ from the ER for mitochondrial uptake via the MCU. HEK293 cells are reported to show limited ER–mitochondrial Ca²⁺ transfer, but tight IP₃R-dependent ERMCS (Katona et al., 2022). HEK293 cells lacking IP₃R showed fewer regions of close apposition (<20 nm) between the ER and mitochondria and re-expression of IP₃Rs including a non-Ca²⁺ conducting mutant in HEK293 cells rescued the contacts between the ER and mitochondria (Katona et al., 2022). Recruitment of mobile IP₃R at ER–mitochondrial contacts has been shown to license the IP₃Rs to rapidly deliver Ca²⁺ to the mitochondria and increase the activity of mitochondrial Ca²⁺-sensitive dehydrogenases (Katona et al., 2022). Analysis using drug-inducible ER–mitochondrial linkers showed that ERMCS junctions formed more quickly in WT DT40 cells compared to IP₃R knock-out cells where IP₃R-mediated Ca²⁺ release was completely attenuated (Bartok et al., 2019). This further suggests that IP₃R are not situated at junctions with mitochondria solely for the provision of Ca²⁺, but are required for junction assembly. IP₃R-mediated regulation of the gap length at specialised junctions is independent of their Ca²⁺ flux property as pore-dead IP₃Rs are equally as competent in restoring tight junctions. Close ER–mitochondria proximity nonetheless remains key for Ca²⁺ transfer to the mitochondria as forcing the mitochondria to the PM and away from the ER disrupts IP₃R-mediated Ca²⁺ transfer into the mitochondria. Such proximity is likely maintained by several tethering complexes acting synergistically.

Co-Regulators of the IP₃R-GRP75-VDAC1 complex at ERMCS

Although the IP₃R–VDAC–GRP75 interaction in the canonical tethering complex is often reported at the ERMCS Ca²⁺ microdomain, these junctions can be modulated by other membrane-anchored or cytosolic binding partners (Figure 2).

Figure 2.

Composition of ER–Mitochondrial Contact Sites Where Ca²⁺ Exchange Occurs. Various binding partners and regulators of the IP₃R-GRP75-VDAC1 complex have been described. These act either by binding directly to one or more of the components (top) or by stabilising the ERMCS (bottom) thereby allowing IP₃R to remain localised preferentially to provide Ca²⁺ for the MCU.

Tespa1

Thymocyte-expressed positive selection-associated gene 1 (Tespa1) is an important regulator of T cell development in the thymus which shares sequence homology (including the double phenylalanine motif that interacts with IP₃Rs) with KRAP (Matsuzaki et al., 2012, Thillaiappan et al., 2021). Although it lacks the actin-binding domain of KRAP, Tespa1 has been shown to interact with IP₃Rs as well as GRP75 in T and B lymphocytes (Matsuzaki et al., 2012, Matsuzaki et al., 2013). The loss of Tespa1 leads to a reduction in both cytosolic and mitochondrial Ca²⁺ signals following T-cell receptor stimulation (Matsuzaki et al., 2013). This would suggest that Tespa1 functions in these cells to regulate IP₃R activity at ERMCS (Matsuzaki et al., 2012).

Sigma-1 Receptor

The ER chaperone protein sigma-1 receptor is an important regulator of Ca²⁺ signalling at ERMCS. The sigma-1 receptor is thought to remain bound to another chaperone protein binding immunoglobulin protein (BiP) under physiological conditions. However, upon ER stress, such as during ER Ca²⁺ depletion, sigma-1 dissociates from BiP and translocates to ERMCS, stabilising IP₃R3 at these junctions and promoting the prolonged release of Ca²⁺ from the ER into the mitochondria (Hayashi and Su, 2007). Other functions of the sigma-1 receptor at ERMCS include stabilising the ER stress sensor inositol-requiring enzyme 1 (IRE-1) and promoting its ability to respond to mitochondria-derived reactive oxygen species during ER stress (Mori et al., 2013). Furthermore, the sigma-1 receptor may regulate dendritic spine formation by modulating the free radical formation around ERMCS to dampen caspase-3 activation and consequent inactivation of Rac GTPase via the degradation of guanine nucleotide exchange factor (Tsai et al., 2009). Sigma-1 receptors functioning at ERMCS are implicated in the pathophysiology of multiple neurodegenerative diseases (Nguyen et al., 2017).

BOK

B cell lymphoma 2 (BCL-2) ovarian killer (BOK) is an ER-resident pro-apoptotic protein. It has been shown to bind IP₃Rs, regulating both Ca²⁺ dynamics at ERMCS under physiological conditions, and following a stimulus (Carpio et al., 2021). BOK is also shown to regulate the stability and composition of proteins at ERMCS (Carpio et al., 2021). The loss of BOK leads to a decreased localisation of IP₃R1, IP₃R3 and the sigma-1 receptor at ERMCS as assessed by microscopy (Carpio et al., 2021). Furthermore, whilst the amount of VDAC1 is unchanged following the loss of BOK in pure mitochondria-associated membrane (MAM) fractions, the amount of IP₃R1 and IP₃R3 is significantly attenuated (Carpio et al., 2021). Conversely, overexpression of MCL-1 and BOK transmembrane domains in HeLa cells increases ERMCS populated by IP₃R and VDAC as assessed via proximity ligation assay (Lucendo et al., 2020). The BOK-IP₃R interaction at ERMCS is implicated in the regulation of apoptosis in these cells (Carpio et al., 2021). Altogether, these suggest BOK plays a role in the recruitment and localisation of IP₃R at ERMCS.

Tom70

The translocase of the outer membrane (TOM) family of proteins accounts for a significant proportion of the OMM protein content. TOM proteins, together with the translocase of the inner membrane (TIM), are key regulators of the trafficking of mitochondrial proteins encoded in the nucleus (Chacinska et al., 2009). Unlike TOM20 which has a uniform distribution across the OMM, TOM70 appears more punctate, coinciding with ERMCS contact sites identified with an engineered split-GFP construct (Filadi et al., 2018). TOM70 was also found to be present in pure MAM fractions physically bind to IP₃R2 (as assessed by co-immunoprecipitation analysis) and regulate IP₃R-mediated Ca²⁺ transfer and cellular bioenergetics (Filadi et al., 2018). Of note, the depletion of TOM70 did not affect the number of ERMCS junctions detected (Filadi et al., 2018). This suggests that TOM70 is not structurally required in the assembly of the junction but may be involved in ‘trapping’ IP₃R at these junctions.

Transglutaminase Type 2 (TG2)

Transglutaminase type 2 (TG2) is an important mediator of post-translational modifications of proteins and localises to various cellular compartments including the cytosol, mitochondria and nucleus (Szondy et al., 2017). Mass spectrometry reveals that TG2 interacts with multiple proteins on the ER and mitochondria including BiP, TOM70 and GRP75 (D’Eletto et al., 2018). TG2 was found to immunoprecipitate with GRP75 and was also enriched in pure MAM fractions (D’Eletto et al., 2018). Overexpression of TG2 reduces the IP₃R3–GRP75 interaction in MEFs, suggesting that TG2 is a negative regulator of IP₃R-GRP75 (D’Eletto et al., 2018). TG2 also modulates the composition of ERMCS and mitochondrial Ca²⁺ uptake following agonist stimulation (D’Eletto et al., 2018). Although the IP₃R3–GRP75 association is increased in the absence of TG2, the overall ERMCS number is reduced (D’Eletto et al., 2018). One argument is that the increase in the IP₃R3–GRP75 interaction occurs as a compensatory mechanism for the reduction in physical coupling between the ER and mitochondria. It remains to be elucidated if TG2 is a physical tether or only a regulatory partner at these ERMCS.

iRhom

iRhom, a catalytically-dead member of the rhomboid family of serine proteases is best characterised as a cofactor of ADAM metallopeptidase domain 17 (ADAM17) (Dulloo et al., 2019). Although not exclusively, iRhoms which has 2 subtypes (iRhom1/2) can be found on the ER and have been implicated in regulating the transfer of Ca²⁺ to the mitochondria via IP₃Rs in ER stress (Dulloo et al., 2022). Depletion of iRhom1/2 under ER stress leads to defective mitochondrial membrane potential and abrogated IP₃R-mediated cytosolic and mitochondrial Ca²⁺ signals (Dulloo et al., 2022). Although iRhoms were shown to interact with IP₃Rs, it is not clear if they reside within ERMCS, or if they indirectly affect mitochondrial Ca²⁺ uptake by globally attenuating IP₃R activity under ER stress.

Seipin

Seipin is an ER resident protein implicated in lipodystrophy (Combot et al., 2022). The observation that patient-derived lymphoblast showed perturbed mitochondrial morphology prompted further investigation into the possible roles of this protein at ERMCS (Combot et al., 2022). Seipin is reported to be enriched at ERMCS populated by IP₃R, VDAC and the sarco/endoplasmic reticulum Ca²⁺-ATPase (SERCA) during starvation (Combot et al., 2022). Seipin deficiency leads to attenuation of the uptake of mitochondrial Ca²⁺ following IP₃R stimulation and impaired mitochondrial metabolism and dynamics in A431 cells (Combot et al., 2022).

Pyruvate Dehydrogenase Kinases (PDKs)

Pyruvate dehydrogenase kinases (PDKs) are serine/threonine kinases mainly located in the mitochondria where they regulate the activity of pyruvate dehydrogenase (PDH) in glycolysis (Wang et al., 2021). There are four isoforms of PDK (PDK 1-4), showing broad tissue expression. Using proximity ligation assays and cellular fractionation analysis, PDK4 alone was shown to localise and interact with IP₃R1–VDAC–GRP75 at ERMCS in skeletal muscle cells (Thoudam et al., 2018). Overexpression of PDK4 enhances the IP₃R1–VDAC1–GRP75 interaction at ERMCS (Thoudam et al., 2018). The kinase activity of PDK4 is required for this enhancement as the pharmacological inhibition of its kinase activity, or the use of a kinase-dead mutant, does not show this enhancement (Thoudam et al., 2018). Inhibition of PDK4 in these cells also attenuates IP₃R-mediated Ca²⁺ transfer to the mitochondria without affecting the ER store content (Thoudam et al., 2018). Using mice models, PDK4 has been shown to be increased in obesity, leading to the regulation of ERMCS formation and Ca²⁺ transfer to the mitochondria (Thoudam et al., 2018). This suggests PDK4 as a structural tether and stabiliser of IP₃R-positive ERMCS in these specialised cells. PDKs are traditionally known to reside in the mitochondrial matrix (Wang et al., 2021), therefore the mechanism by which they interact and couple with IP₃R1, GRP75 and VDAC in MAM fractions remains unclear. Furthermore, PDK4 can be found in other cell types such as the liver, kidneys and pancreatic cells (Moon et al., 2012), but it remains to be determined if it also interacts at ERMCS in these other cells.

Other Co-Regulators of ERMCS Where Ca²⁺ Exchange Occurs

Other regulators of ERMCS important for Ca²⁺ exchange have been described in metabolic regulation, neurodegenerative disease models and in cancer. In metabolic regulation, abrogation of the tethers VAPB on the ER membrane and protein tyrosine phosphatase interacting protein 51 (PTPIP51) on the mitochondrial membrane at ERMCS that regulate autophagy attenuates the IP₃R3–VDAC1 interaction and subsequent IP₃R mediated Ca²⁺ signals to the mitochondria (Gomez-Suaga et al., 2017). Furthermore, the deletion of PTP1P51 coiled-coil domain which affects its localisation at ERMCS, abrogates IP₃R-mediated Ca²⁺ delivery to the mitochondria (Mórotz et al., 2022). In cardiomyocytes, the mitochondrial resident protein FUN14 domain containing 1 (FUNDC1) binds to IP₃R2 to regulate ERMCS and modulate Ca²⁺ exchange at these junctions (Wu et al., 2017).

In neurodegenerative disease models, the loss of function mutation in Niemann-Pick type C1 (NPC1) changes the spatial distribution of IP₃R1, potentiates GPCR-mediated Ca²⁺ signals, and promotes IP₃R-mediated mitochondrial Ca²⁺ uptake causing cytotoxicity (Tiscione et al., 2021). Furthermore, DJ-1 encoded for by PARK7 is a cytosolic and nuclear protein implicated in early onset Parkinson's disease. PARK7 which translocates to ERMCS to interact with the IP₃R–GRP75–VDAC1 complex to regulate both the integrity of the ERMCS and Ca²⁺ transfer at these junctions (Liu et al., 2019). DJ1 regulates the spatial localisation of IP₃R, with the loss of DJ1, leading to the aggregation of IP₃R via an unknown mechanism (Liu et al., 2019). Actin polymerisation via the ER-anchored inverted formin 2 (INF2) results in an increase in mitochondrial Ca²⁺ and further change in the mitochondrial morphology via dynamin-related protein 1 (Drp1) recruitment (Chakrabarti et al., 2018).

In adrenocortical carcinoma, fetal and adult testis-expressed 1 (FATE1), which encodes a cancer-testis antigen has been shown to localise to MAMs (Doghman-Bouguerra et al., 2016). There it decreases ERMCS and negatively modulates the transfer of IP₃R-mediated Ca²⁺ to the mitochondria. In addition, Furthermore, FATE1 has also been suggested to attenuate apoptosis in these adrenocortical carcinoma cells (Doghman-Bouguerra et al., 2016). Upregulation of FATE1 in cancers may occur to drive uncoupling of the ER and mitochondria, attenuate Ca²⁺ delivery and increase the resistance to cell death in these cancer cells.

Perspectives and Concluding Remarks

As highlighted, multiple regulators of the IP₃R-GRP75-VDAC complex at ERMCS mediate Ca²⁺ exchange in various cell types. This poses the question of how ERMCS are regulated? Furthermore, the dogma that the IP₃R–GRP75–VDAC tripartite complex forms a funnel through which Ca²⁺ is exchanged from the ER to the mitochondria, requires closer scrutiny. Firstly, there is evidence that other binding partners can interact with IP₃R to form ERMCS where Ca²⁺ exchange occurs, as is the case for IP₃R interacting with the AKAP1 transmembrane domain or TOM70 on the mitochondrial membrane (Katona et al., 2022). Although it is important to note that TOM70 has been shown to interact directly with the IP₃R–GRP75–VDAC complex (SECTION 3.3); therefore, TOM70 may well be a coincident reporter of the same IP₃R–GRP75–VDAC junctions. Secondly, disruption of other tethering complexes such as between VAPB and PTPIP51 on the OMM, also disrupts Ca²⁺ uptake into the mitochondria (De Vos et al., 2012). This suggests a model where tethering complexes work synergistically to promote ERMCS where Ca²⁺ exchange can occur. Indeed, overexpression of VAPB and PTP1P51 was shown to increase the IP₃R3–VDAC1 interaction in HeLa cells and Ca²⁺ exchange at these junctions (Gomez-Suaga et al., 2017). Furthermore, the knockdown of mitofusin-2 which tethers at ERMCS via interaction with either mitofusin 1 or 2 on the OMM also regulates the stability of the junctions, with its ablation leading to attenuated mitochondrial Ca²⁺ uptake (de Brito and Scorrano, 2008, Naon et al., 2016). This is not without controversy, since MFN2 has been postulated to act in the opposite direction where it reduces the stability of ERMCS and its reduction increases Ca²⁺ transfer to the mitochondria (Filadi et al., 2015). Thirdly, local Ca²⁺ transfer at ERMCS can be circumvented using a sufficiently high concentration of a rapid Ca²⁺ chelating agent such as ethylene glycol-bis(β-aminoethyl ether)-N,N,N′,N′-tetraacetic acid (EGTA) (Csordas et al., 1999). A tight Ca²⁺ microdomain synonymous with a funnel may be impenetrable by buffering agents as Ca²⁺ can be channelled through to the mitochondria regardless of the buffering activity in the cytosol.

We suggest that the ER–mitochondrial Ca²⁺ microdomain is maintained by a complex of different tethers and binding proteins working synergistically to promote ERMCS contact sites where IP₃R sits to power the low-affinity mitochondrial Ca²⁺ uptake mechanism. Further work is required to elucidate the interdependence of various tethering complexes on mitochondrial Ca²⁺ uptake and their role in health and disease. Whilst current technologies are limited in their capabilities, it is also important to assess how the individual tethering complexes at ERMCS change simultaneously in response to metabolic demands, as it will help answer the longstanding questions around the role of the multiple complexes found at these junctions.

Footnotes

Abbreviations

Acknowledgement

This work was supported by the Biotechnology and Biological Sciences Research Council UK (BB/T012986/1). P.A-A is a fellow of Emmanuel College, Cambridge. A.I is supported by a Cambridge European, Department of Pharmacology & Wolfson Medical Research Scholarship. We would like to thank Prof Colin Taylor for helpful discussions during the write-up of this review. We thank Prof Graham Ladds for providing guidance and for reviewing the manuscript to enhance its clarity.

Declaration of Conflicting Interests

The author(s) declared no potential conflicts of interest with respect to the research, authorship, and/or publication of this article.

ORCID iDs

Peace Atakpa-Adaji

References

Alzayady

Chandrasekhar

Yule

(2013). Fragmented inositol 1,4,5-trisphosphate receptors retain tetrameric architecture and form functional Ca²⁺ release channels. Journal of Biological Chemistry 288(16), 11122–11134. https://doi.org/10.1074/jbc.M113.453241

Area-Gomez

de Groof

Boldogh

Bird

Gibson

Koehler

Duff

Yaffe

Pon

et al. (2009). Presenilins are enriched in endoplasmic reticulum membranes associated with mitochondria. The American Journal of Pathology 175(5), 1810–1816.

Arige

Terry

Malik

Knebel

Wagner Ii

Yule

(2021). CREB Regulates the expression of type 1 inositol 1,4,5-trisphosphate receptors. Journal of Cell Science 134(20), jcs258875.

Baines

Kaiser

Sheiko

Craigen

Molkentin

(2007). Voltage-dependent anion channels are dispensable for mitochondrial-dependent cell death. Nature Cell Biology 9(5), 550–555.

Bartok

Weaver

Golenar

Nichtova

Katona

Bansaghi

Alzayady

Thomas

Ando

Mikoshiba

et al. (2019). IP₃ Receptor isoforms differently regulate ER-mitochondrial contacts and local calcium transfer. Nature Communications 10(1), 3726.

Berridge

(1997) Elementary and global aspects of calcium signalling. Journal of Physiology 499, 291–306.

Berridge

(2016). The inositol trisphosphate/calcium signaling pathway in health and disease. Physiological Reviews 96, 1261–1296.

Berridge

Lipp

Bootman

(2000). The versatility and universality of calcium signalling. Nature Reviews: Molecular Cell Biology 1, 11–21.

Blondel

Bell

Moody

Miller

Gibbons

(1994). Creation of an inositol 1,4,5-trisphosphate-sensitive Ca²⁺ store in secretory granules of insulin-producing cells. Journal of Biological Chemistry 269, 27167–27170.

10.

Bootman

Berridge

(1996). Subcellular Ca²⁺ signals underlying waves and graded responses in HeLa cells. Current Biology 6, 855–865.

11.

Bootman

Berridge

Lipp

(1997). Cooking with calcium: The recipes for composing global signals from elementary events. Cell 91, 367–373.

12.

Bragadin

Pozzan

Azzone

(1979). Kinetics of Ca²⁺ carrier in rat liver mitochondria. Biochemistry 18(26), 5972–5978.

13.

Bravo

Vicencio

Parra

Troncoso

Munoz

Bui

Quiroga

Rodriguez

Verdejo

Ferreira

et al. (2011). Increased ER-mitochondrial coupling promotes mitochondrial respiration and bioenergetics during early phases of ER stress. Journal of Cell Science 124(Pt 13), 2143–2152.

14.

Carpio

Means

Brill

Sainz

Ehrlich

Katz

(2021). BOK Controls apoptosis by Ca²⁺ transfer through ER-mitochondrial contact sites. Cell Reports 34(10), 108827.

15.

Chacinska

Koehler

Milenkovic

Lithgow

Pfanner

(2009). Importing mitochondrial proteins: machineries and mechanisms. Cell 138(4), 628–644.

16.

Chakrabarti

Stan

de Juan Sanz

Ryan

Higgs

(2018). INF2-mediated Actin polymerization at the ER stimulates mitochondrial calcium uptake, inner membrane constriction, and division. Journal of Cell Biology 217, 251–268.

17.

Chang

Liou

(2016). Homeostatic regulation of the PI(4,5)P₂-Ca²⁺ signaling system at ER-PM junctions. Biochimica et Biophysica Acta 1861, 862–873.

18.

Clapham

(2007). Calcium signaling. Cell 131, 1047–1058.

19.

Combot

Salo

Chadeuf

Hölttä

Ven

Pulli

Ducheix

Pecqueur

Renoult

Lak

et al. (2022). Seipin localizes at endoplasmic-reticulum-mitochondria contact sites to control mitochondrial calcium import and metabolism in adipocytes. Cell Reports 38(2), 110213.

20.

Copeland

Dalton

(1959). An association between mitochondria and the endoplasmic reticulum in cells of the pseudobranch gland of a teleost. The Journal of Biophysical and Biochemical Cytology 5(3), 393–396.

21.

Csordas

Thomas

Hajnóczky

(1999). Quasi-synaptic calcium signal transmission between endoplasmic reticulum and mitochondria. European Molecular Biology Organisation Journal 18, 96–108.

22.

Csordas

Varnai

Golenar

Roy

Purkins

Schneider

Balla

Hajnoczky

(2010). Imaging interorganelle contacts and local calcium dynamics at the ER-mitochondrial interface. Molecular Cell 39, 121–132.

23.

Deak

Blass

Khan

Groschner

Waldeck-Weiermair

Hallström

Graier

Malli

(2014). IP₃-mediated STIM1 oligomerization requires intact mitochondrial Ca²⁺ uptake. Journal of Cell Science 127(Pt 13), 2944–2955.

24.

de Brito

Scorrano

(2008). Mitofusin 2 tethers endoplasmic reticulum to mitochondria. Nature 456(7222), 605–610.

25.

de Brito

Scorrano

(2010). An intimate liaison: Spatial organization of the endoplasmic reticulum-mitochondria relationship. European Molecular Biology Organisation Journal 29(16), 2715–2723.

26.

D’Eletto

Rossin

Occhigrossi

Farrace

Faccenda

Desai

Marchi

Refolo

Falasca

Antonioli

et al. (2018). Transglutaminase type 2 regulates ER-mitochondria contact sites by interacting with GRP75. Cell Reports 25(13), 3573–3581.e3574.

27.

Dellis

Dedos

Tovey

Rahman

T-U-

Dubel

Taylor

(2006). Ca²⁺ entry through plasma membrane IP₃ receptors. Science 313, 229–233.

28.

Denton

(2009). Regulation of mitochondrial dehydrogenases by calcium ions. Biochimica et Biophysica Acta 1787(11), 1309–1316.

29.

De Smedt

Missiaen

Parys

Henning

Sienaert

Vanlingen

Gijsens

Himpens

Caseels

(1997). Isoform diversity of the inositol trisphosphate receptor in cell types of mouse origin. Biochemical Journal 322, 575–583.

30.

De Stefani

Bononi

Romagnoli

Messina

De Pinto

Pinton

Rizzuto

(2012). VDAC1 Selectively transfers apoptotic Ca²⁺ signals to mitochondria. Cell Death & Differentiation 19(2), 267–273.

31.

De Stefani

Patron

Rizzuto

(2015). Structure and function of the mitochondrial calcium uniporter complex. Biochimica et Biophysica Acta (BBA) - Molecular Cell Research 1853(9), 2006–2011.

32.

De Stefani

Raffaello

Teardo

Szabo

Rizzuto

(2011). A forty-kilodalton protein of the inner membrane is the mitochondrial calcium uniporter. Nature 476, 336–340.

33.

De Vos

Mórotz

Stoica

Tudor

Lau

Ackerley

Warley

Shaw

Miller

(2012). VAPB Interacts with the mitochondrial protein PTPIP51 to regulate calcium homeostasis. Human Molecular Genetics 21(6), 1299–1311.

34.

Doghman-Bouguerra

Granatiero

Sbiera

Lacas-Gervais

Brau

Fassnacht

Rizzuto

Lalli

(2016). FATE1 Antagonizes calcium- and drug-induced apoptosis by uncoupling ER and mitochondria. EMBO Reports 17(9), 1264–1280.

35.

Dulloo

Atakpa-Adaji

Yeh

Levet

Muliyil

Taylor

Freeman

(2022). Irhom pseudoproteases regulate ER stress-induced cell death through IP₃ receptors and BCL-2. Nature Communications 13(1), 1257.

36.

Dulloo

Muliyil

Freeman

(2019). The molecular, cellular and pathophysiological roles of iRhom pseudoproteases. Open Biology 9(3), 190003.

37.

Fernandez-Busnadiego

Saheki

De Camilli

(2015). Three-dimensional architecture of extended synaptotagmin-mediated endoplasmic reticulum-plasma membrane contact sites. Proceedings of the National Academy of Sciences 112, E2004–E2013.

38.

Filadi

Greotti

Turacchio

Luini

Pozzan

Pizzo

(2015). Mitofusin 2 ablation increases endoplasmic reticulum-mitochondria coupling. Proceedings of the National Academy of Sciences 112(17), E2174–E2181.

39.

Filadi

Leal

Schreiner

Rossi

Dentoni

Pinho

Wiehager

Cieri

Calì

Pizzo

et al. (2018). TOM70 Sustains cell bioenergetics by promoting IP₃R3-mediated ER to mitochondria Ca²⁺ transfer. Current Biology 28(3), 369–382.e366.

40.

Foskett

White

Cheung

Mak

(2007). Inositol trisphosphate receptor Ca²⁺ release channels. Physiological Reviews 87, 593–658.

41.

Gincel

Zaid

Shoshan-Barmatz

(2001). Calcium binding and translocation by the voltage-dependent anion channel: A possible regulatory mechanism in mitochondrial function. Biochemical Journal 358(Pt 1), 147–155.

42.

Giorgi

Marchi

Pinton

(2018). The machineries, regulation and cellular functions of mitochondrial calcium. Nature Reviews Molecular Cell Biology 19(11), 713–730.

43.

Gomez-Suaga

Paillusson

Stoica

Noble

Hanger

Miller

CCJ

(2017). The ER-mitochondria tethering Complex VAPB-PTPIP51 regulates autophagy. Current Biology 27(3), 371–385.

44.

Guardia-Laguarta

Area-Gomez

Rüb

Liu

Magrané

Becker

Voos

Schon

Przedborski

(2014). α-Synuclein is localized to mitochondria-associated ER membranes. The Journal of Neuroscience 34(1), 249–259.

45.

Hayashi

(2007). Sigma-1 receptor chaperones at the ER-mitochondrion interface regulate Ca²⁺ signaling and cell survival. Cell 131(giot), 596–610.

46.

Iwai

Michikawa

Bosanac

Ikura

Mikoshiba

(2007). Molecular basis of the isoform-specific ligand-binding affinity of inositol 1,4,5-trisphosphate receptors. Journal of Biological Chemistry 282, 12755–12764.

47.

Kaftan

Ehrlich

Watras

(1997). Inositol 1,4,5-trisphosphate (InsP₃) and calcium interact to increase the dynamic range of InsP₃ receptor-dependent calcium signaling. Journal of General Physiology 110, 529–538.

48.

Katona

Bartók

Nichtova

Csordás

Berezhnaya

Weaver

Ghosh

Várnai

Yule

Hajnóczky

(2022). Capture at the ER-mitochondrial contacts licenses IP₃ receptors to stimulate local Ca²⁺ transfer and oxidative metabolism. Nature Communications 13(1), 6779.

49.

Kim

Moody

Edgerly

Bordiuk

Cormier

Smith

Beal

Ferrante

(2010). Mitochondrial loss, dysfunction and altered dynamics in Huntington's disease. Human Molecular Genetics 19(20), 3919–3935.

50.

Kim

Coukos

Gao

Krainc

(2022). Dysregulation of organelle membrane contact sites in neurological diseases. Neuron 110(15), 2386–2408.

51.

Kirichok

Krapavinsky

Clapham

(2004). The mitochondrial calcium uniporter is a highly selective ion channel. Nature 427, 360–364.

52.

Klar

Hisatsune

Baig

Tariq

Johansson

Rasool

Malik

Ameur

Sugiura

Feuk

Mikoshiba

Dahl

(2014). Abolished InsP₃R2 function inhibits sweat secretion in both humans and mice. Journal of Clinical Investigation 124, 4773–4780.

53.

Kohler

Aufschnaiter

Büttner

(2020). Closing the Gap, Membrane Contact Sites in the Regulation of Autophagy. Cells 9(5).

54.

Kroemer

Reed

(2000). Mitochondrial control of cell death. Nature Medicine 6(5), 513–519.

55.

Liu

Fujioka

Liu

Chen

Zhu

(2019). DJ-1 regulates the integrity and function of ER-mitochondria association through interaction with IP3R3-Grp75-VDAC1. Proceedings of the National Academy of Sciences 116(50), 25322–25328.

56.

Lock

Alzayady

Yule

Parker

(2018). All three IP₃ receptor isoforms generate Ca²⁺ puffs that display similar characteristics. Science Signaling 11, eaau0344.

57.

Lock

Parker

(2020). IP₃ Mediated global Ca²⁺ signals arise through two temporally and spatially distinct modes of Ca²⁺ release. Elife 9, e55008.

58.

Lopez-Crisosto

Díaz-Vegas

Castro

Rothermel

Bravo-Sagua

Lavandero

(2021). Endoplasmic reticulum-mitochondria coupling increases during doxycycline-induced mitochondrial stress in HeLa cells. Cell Death Discovery 12(7), 657.

59.

Lucendo

Sancho

Lolicato

Javanainen

Kulig

Leiva

Duart

Andreu-Fernández

Mingarro

Orzáez

(2020). Mcl-1 and Bok transmembrane domains: Unexpected players in the modulation of apoptosis. Proceedings of the National Academy of Sciences 117(45), 27980–27988.

60.

Mak

Foskett

(1998). Effects of divalent cations on single-channel conduction properties of Xenopus IP₃ receptor. American Journal of Physiology 275, C179–C188.

61.

Mak

D-O

McBride

Foskett

(2001). Regulation by Ca²⁺ and inositol 1,4,5-trisphosphate (InsP₃) of single recombinant type 3 InsP₃ receptor channels: Ca²⁺ activation uniquely distinguishes types 1 and 3 InsP₃ receptors. Journal of General Physiology 117, 435–446.

62.

Malviya

Rogue

Vincendon

(1990). Stereospecific inositol 1,4,5-[³²P]trisphosphate binding to isolated rat liver nuclei: Evidence for inositol trisphosphate receptor-mediated calcium release from the nucleus. Proceedings of the National Academy of Sciences 87, 9270–9274.

63.

Marchant

Callamaras

Parker

(1999). Initiation of IP₃-mediated Ca²⁺ waves in Xenopus oocytes. European Molecular Biology Organisation Journal 18, 5285–5299.

64.

Mataragka

Taylor

(2018). All three IP₃ receptor subtypes generate Ca²⁺ puffs, the universal building blocks of IP₃-evoked Ca²⁺ signals. Journal of Cell Science 131, jcs220848.

65.

Matsuzaki

Fujimoto

Ota

Ogawa

Tsunoda

Doi

Hamabashiri

Tanaka

Shirasawa

(2012). Tespa1 is a novel inositol 1,4,5-trisphosphate receptor binding protein in T and B lymphocytes. FEBS Open Biology 2, 255–259.

66.

Matsuzaki

Fujimoto

Tanaka

Shirasawa

(2013). Tespa1 is a novel component of mitochondria-associated endoplasmic reticulum membranes and affects mitochondrial calcium flux. Biochemical and Biophysical Research Communications 433, 322–326.

67.

Mendes

Gomes

Thompson

Souto

Goes

Rodrigues

Gomez

Nathanson

Leite

(2005). The type III inositol 1,4,5-trisphosphate receptor preferentially transmits apoptotic Ca²⁺ signals into mitochondria. Journal of Biological Chemistry 280, 40892–40900.

68.

Milakovic

Quintanilla

Johnson

(2006). Mutant huntingtin expression induces mitochondrial calcium handling defects in clonal striatal cells: Functional consequences. Journal of Biological Chemistry 281(46), 34785–34795.

69.

Miyakawa

Maeda

Yamazawa

Hirose

Kurosaki

Iino

(1999). Encoding of Ca²⁺ signals by differential expression of IP₃ receptor subtypes. European Molecular Biology Organisation Journal 18, 1303–1308.

70.

Moon

Lee

Kim

Jeoung

Lee

Kim

(2012). Association of pyruvate dehydrogenase kinase 4 gene polymorphisms with type 2 diabetes and metabolic syndrome. Diabetes Research and Clinical Practice 95(2), 230–236.

71.

Mori

Hayashi

(2013). Sigma-1 receptor chaperone at the ER-mitochondrion interface mediates the mitochondrion-ER-nucleus signaling for cellular survival. PLoS One 8(10), e76941.

72.

Mórotz

Martín-Guerrero

Markovinovic

Paillusson

Russell

MRG

Machado

PMP

Fleck

Noble

Miller

CCJ

(2022). The PTPIP51 coiled-coil domain is important in VAPB binding, formation of ER-mitochondria contacts and IP3 receptor delivery of Ca2 + to mitochondria. Frontiers in Cell and Developmental Biology 10.

73.

Nakanishi

Maeda

Mikoshiba

(1991). Immunohistochemical localization of an inositol 1,4,5-trisphosphate receptor, P400, in neural tissue: Studies in developing and adult mouse brain. The Journal of Neuroscience 11(7), 2075.

74.

Nan

Lin

Saif Ur Rahman

Zhu

(2021). The interplay between mitochondria and store-operated Ca²⁺ entry: Emerging insights into cardiac diseases. Journal of Cellular and Molecular Medicine 25(20), 9496–9512.

75.

Naon

Zaninello

Giacomello

Varanita

Grespi

Lakshminaranayan

Serafini

Semenzato

Herkenne

Hernández-Alvarez

et al. (2016). Critical reappraisal confirms that Mitofusin 2 is an endoplasmic reticulum-mitochondria tether. Proceedings of the National Academy of Sciences 113(40), 11249–11254.

76.

Newton

Mignery

Südhof

(1994). Co-expression in vertebrate tissues and cell lines of multiple inositol 1,4,5-trisphosphate (InsP₃) receptors with distinct affinities for InsP₃. Journal of Biological Chemistry 269, 28613–28619.

77.

Nguyen

Lucke-Wold

Mookerjee

Kaushal

Matsumoto

(2017). Sigma-1 receptors and neurodegenerative diseases: Towards a hypothesis of sigma-1 receptors as amplifiers of neurodegeneration and neuroprotection. Advances in Experimental Medicine and Biology 964, 133–152.

78.

Obara

Nixon-Abell

Moore

Riccio

Hoffman

Shtengel

Schaefer

Pasolli

Masson

J-B

et al. (2022). Motion of single molecular tethers reveals dynamic subdomains at ER-mitochondria contact sites. bioRxiv: 2022.2009.2003.505525.

79.

Parker

Choi

Yao

(1996). Elementary events of InsP₃-induced Ca²⁺ liberation in Xenopus oocytes: Hot spots, puffs and blips. Cell Calcium 20, 105–121.

80.

Pinton

Pozzan

Rizzuto

(1998). The Golgi apparatus is an inositol 1,4,5-trisphosphate-sensitive Ca²⁺ store, with functional properties distinct from those of the endoplasmic reticulum. European Molecular Biology Organisation Journal 17, 5298–5308.

81.

Pinton

Rizzuto

(2006). Bcl-2 and Ca²⁺ homeostasis in the endoplasmic reticulum. Cell Death & Differentiation 13(8), 1409–1418.

82.

Prinz

(2014). Bridging the gap: Membrane contact sites in signaling, metabolism, and organelle dynamics. Journal of Cell Biology 205, 759–769.

83.

Prole

Taylor

(2016). Inositol 1,4,5-trisphosphate receptors and their protein partners as signalling hubs. Journal of Physiology 594, 2849–2866.

84.

Qin

Guo

Xue

Shi

Chen

Hao

Zhao

Sun

(2020). ER-mitochondria contacts promote mtDNA nucleoids active transportation via mitochondrial dynamic tubulation. Nature Communications 11(1), 4471.

85.

Rapizzi

Pinton

Szabadkai

Wieckowski

Vandecasteele

Baird

Tuft

Fogarty

Rizzuto

(2002). Recombinant expression of the voltage-dependent anion channel enhances the transfer of Ca²⁺ microdomains to mitochondria. Journal of Cell Biology 159(4), 613–624.

86.

Rieusset

(2018). The role of endoplasmic reticulum-mitochondria contact sites in the control of glucose homeostasis: An update. Cell Death & Disease 9(3), 388.

87.

Rizzuto

Pinton

Carrington

Fay

Fogarty

Lifshitz

Tuft

Pozzan

(1998). Close contacts with the endoplasmic reticulum as determinants of mitochondrial Ca²⁺ responses. Science 280, 1763–1766.

88.

Sakai

Watanabe

Komine

Sobue

Yamanaka

(2021). Novel reporters of mitochondria-associated membranes (MAM), MAMtrackers, demonstrate MAM disruption as a common pathological feature in amyotrophic lateral sclerosis. The FASEB Journal 35(7), e21688.

89.

Sassano

Felipe-Abrio

Agostinis

(2022). ER-mitochondria contact sites; a multifaceted factory for Ca²⁺ signaling and lipid transport. Frontiers in Cell and Developmental Biology 10.

90.

Schmitz

Takahashi

Karakas

(2022). Structural basis for activation and gating of IP₃ receptors. Nature Communications 13(1), 1408.

91.

Shoshan-Barmatz

Krelin

Shteinfer-Kuzmine

(2018). VDAC1 Functions in Ca²⁺ homeostasis and cell life and death in health and disease. Cell Calcium 69, 81–100.

92.

Simoes

ICM

Morciano

Lebiedzinska-Arciszewska

Aguiari

Pinton

Potes

Wieckowski

(2020). The mystery of mitochondria-ER contact sites in physiology and pathology: A cancer perspective. Biochimica et Biophysica Acta (BBA) - Molecular Basis of Disease 1866(10), 165834.

93.

Simpson

Mehotra

Langley

Sheppard

(1998). Specialized distributions of mitochondria and endoplasmic reticulum proteins define Ca²⁺ wave amplification sites in cultured astrocytes. Journal of Neuroscience Research 52(6), 672–683.

94.

Sood

Jeyaraju

Prudent

Caron

Lemieux

McBride

Laplante

Tóth

Pellegrini

(2014). A Mitofusin-2 dependent inactivating cleavage of Opa1 links changes in mitochondria cristae and ER contacts in the postprandial liver. Proceedings of the National Academy of Sciences 111(45), 16017–16022.

95.

Sukumaran

Nascimento Da Conceicao

Sun

Ahamad

Saraiva

Selvaraj

Singh

(2021). Calcium Signaling Regulates Autophagy and Apoptosis. Cells 10.

96.

Szabadkai

Bianchi

Varnai

De Stefani

Wieckowski

Cavagna

Nagy

Balla

Rizzuto

(2006). Chaperone-mediated coupling of endoplasmic reticulum and mitochondrial Ca²⁺ channels. Journal of Cell Biology 175, 901–911.

97.

Szondy

Korponay-Szabó

Király

Sarang

Tsay

(2017). Transglutaminase 2 in human diseases. Biomedicine (Taipei) 7(3), 15.

98.

Taylor

Genazzani

Morris

(1999). Expression of inositol trisphosphate receptors. Cell Calcium 26, 237–251.

99.

Theurey

Tubbs

Vial

Jacquemetton

Bendridi

Chauvin

Alam

Le Romancer

Vidal

Rieusset

(2016). Mitochondria-associated endoplasmic reticulum membranes allow adaptation of mitochondrial metabolism to glucose availability in the liver. Journal of Molecular Cell Biology 8, 129–143.

100.

Thillaiappan

Chavda

Tovey

Prole

Taylor

(2017). Ca²⁺ signals initiate at immobile IP₃ receptors adjacent to ER-plasma membrane junctions. Nature Communications 8, 1505.

101.

Thillaiappan

Smith

Atakpa-Adaji

Taylor

(2021). KRAP Tethers IP₃ receptors to actin and licenses them to evoke cytosolic Ca²⁺ signals. Nature Communications 12(1), 4514.

102.

Thoudam

C-M

Leem

Chanda

Park

J-S

Kim

H-J

Jeon

J-H

Choi

Y-K

Liangpunsakul

Huh

et al. (2018). PDK4 Augments ER–mitochondria contact to dampen skeletal muscle insulin signaling during obesity. Diabetes 68(3), 571–586.

103.

Tiscione

Casas

Horvath

Lam

Hino

Ory

Santana

Simó

Dixon

Dickson

(2021). IP₃R-driven increases in mitochondrial Ca²⁺ promote neuronal death in NPC disease. Proceedings of the National Academy of Sciences 118(40).

104.

Tsai

Hayashi

Harvey

Wang

Shen

Zhang

Becker

Hoffer

(2009). Sigma-1 receptors regulate hippocampal dendritic spine formation via a free radical-sensitive mechanism involving Rac1xGTP pathway. Proceedings of the National Academy of Sciences 106(52), 22468–22473.

105.

Wang

Bezprozvanny

(2005a). Modulation of mammalian inositol 1,4,5-trisphosphate receptor isoforms by calcium: A role of calcium sensor region. Biophysical Journal 88, 1056–1069.

106.

Wang

Nosyreva

De Smedt

Bezprozvanny

(2005b). Functional characterization of mammalian inositol 1,4,5-trisphosphate receptor isoforms. Biophysical Journal 88, 1046–1055.

107.

Vervloessem

Yule

Bultynck

Parys

(2015). The type 2 inositol 1,4,5-trisphosphate receptor, emerging functions for an intriguing Ca²⁺-release channel. Biochimica et Biophysica Acta 1853, 1992–2005.

108.

Vorontsova

Lock

Parker

(2022). KRAP Is required for diffuse and punctate IP₃-mediated Ca²⁺ liberation and determines the number of functional IP3R channels within clusters. Cell Calcium 107, 102638.

109.

Wagner

2nd Yule

(2012). Differential regulation of the InsP₃ receptor type-1 and −2 single channel properties by InsP₃, Ca²⁺ and ATP. Journal of Physiology 590, 3245–3259.

110.

Wang

Shen

Yan

(2021). Pyruvate dehydrogenase kinases (PDKs): an overview toward clinical applications. Bioscience Reports 41(4).

111.

Wang

Ding

Mao

Huang

Xie

Zou

M-H

(2017). Binding of FUN14 domain containing 1 with inositol 1,4,5-trisphosphate receptor in mitochondria-associated endoplasmic Reticulum membranes maintains mitochondrial dynamics and function in hearts in vivo. Circulation 136(23), 2248–2266.

112.

Guan

Ren

Wei

Tao

Yang

Liu

Zhang

Zhu

(2018). IP₃R-Grp75-VDAC1-MCU Calcium regulation axis antagonists protect podocytes from apoptosis and decrease proteinuria in an Adriamycin nephropathy rat model. BMC Nephrology 19(1), 140.

113.

Wang

Tong

(2020). Endoplasmic Reticulum-mitochondria contact sites and neurodegeneration. Frontiers in Cell and Developmental Biology 8, 428.

114.

Yoneshima

Miyawaki

Michikawa

Furuichi

Mikoshiba

(1997). Ca²⁺ differentially regulates ligand-affinity states of type 1 and 3 inositol 1,4,5-trisphosphate receptors. Biochemical Journal 322, 591–596.

115.

Yoo

Albanesi

(1990). Inositol 1,4,5-trisphosphate-triggered Ca²⁺ release from bovine adrenal medullary secretory vesicles. Journal of Biological Chemistry 265, 13446–13448.

116.

Jin

Huang

(2021). Mitochondria-associated membranes (MAMs): A potential therapeutic target for treating Alzheimer's disease. Clinical Science 135(1), 109–126.

117.

Yuan

Gong

Xie

Zhang

Meng

Tse

Zhao

Bao

Zhang

et al.(2022). IP₃R1/GRP75/VDAC1 complex mediates endoplasmic reticulum stress-mitochondrial oxidative stress in diabetic atrial remodeling. Redox Biology 52, 102289.

118.

Zaman

Nenadic

Radojičić

Rosado

Beh

(2020). Sticking With It: ER-PM Membrane Contact Sites as a Coordinating Nexus for Regulating Lipids and Proteins at the Cell Cortex. Frontiers in Cell and Developmental Biology 8.

119.

Zhang

Fritz

Ibarra

Uhlén

(2011). Inositol 1,4,5-trisphosphate receptor subtype-specific regulation of calcium oscillations. Neurochemical Research 36(7), 1175–1185.

IP 3 R at ER-Mitochondrial Contact Sites: Beyond the IP 3 R-GRP75-VDAC1 Ca 2+ Funnel

Abstract

Keywords

Highlight

Introduction

ERMCS and Ca2+ Signalling

Localisation and Functioning of IP3Rs at ERMCS

All IP3R Subtypes Localise to ER–Mitochondrial Contact Sites

IP3Rs Have a Structural Role in Forming ERMCS

Co-Regulators of the IP3R-GRP75-VDAC1 complex at ERMCS

Tespa1

Sigma-1 Receptor

BOK

Tom70

Transglutaminase Type 2 (TG2)

iRhom

Seipin

Pyruvate Dehydrogenase Kinases (PDKs)

Other Co-Regulators of ERMCS Where Ca2+ Exchange Occurs

Perspectives and Concluding Remarks

Footnotes

Abbreviations

Acknowledgement

Declaration of Conflicting Interests

ORCID iDs

References

ERMCS and Ca²⁺ Signalling

Localisation and Functioning of IP₃Rs at ERMCS

All IP₃R Subtypes Localise to ER–Mitochondrial Contact Sites

IP₃Rs Have a Structural Role in Forming ERMCS

Co-Regulators of the IP₃R-GRP75-VDAC1 complex at ERMCS

Other Co-Regulators of ERMCS Where Ca²⁺ Exchange Occurs