The microRNA hsa-miR-132-5p is upregulated in endometriotic cyst stromal cells (ECSCs). We investigate how hsa-miR-132-5p affects the progression of endometriosis.
Methods:
Normal endometrial stromal cells (NESCs) that have been isolated from normal eutopic endometrium without endometriosis and ECSCs isolated ovarian endometriotic cysts were assayed. We then evaluated how hsa-miR-132-5p impacted apoptosis, motility, and cell viability in hsa-miR-132-5p-transfected NESCs.
Results:
Transfection with hsa-miR-132-5p promoted cell motility and viability and inhibited the apoptosis of NESCs.
Conclusions:
Our results indicate that the expression of hsa-miR-132-5p promotes the acquisition of endometriosis-specific features during the progression of endometriosis. Thus, hsa-miR-132-5p may have potential value in the treatment of endometriosis.
GiudiceLC.Clinical practice. Endometriosis. N Engl J Med2010; 362: 2389–2398.
2.
NasuKYugeATsunoA, et alMevalonate-Ras homology (Rho)/Rho-associated coiled-coil-forming protein kinase (ROCK)-mediated signaling pathway as a therapeutic target for the treatment of endometriosis-associated fibrosis. Curr Signal Transduct Ther2010; 5: 141–148.
3.
NasuKNishidaMKawanoY, et alAberrant expression of apoptosis-related molecules in endometriosis: a possible mechanism underlying the pathogenesis of endometriosis. Reprod Sci2011; 18: 206–218.
4.
AbeWNasuKNakadaC, et almiR-196b targets c-Myc and Bcl-2 expression, inhibits proliferation and induces apoptosis in endometriotic stromal cells. Hum Reprod2013; 28: 750–761.
5.
OkamotoMNasuKAbeW, et alEnhanced miR-210 expression promotes the pathogenesis of endometriosis through activation of signal transducer and activator of transcription 3. Hum Reprod2015; 30: 632–641.
ZhangBPanXCobbGP, et almicroRNAs as oncogenes and tumor suppressors. Dev Biol2007; 302: 1–12.
11.
TanWLiuBQuS, et almicroRNAs and cancer: key paradigms in molecular therapy. Oncol Lett2018; 15: 2735–2742.
12.
LinSGregoryRI.microRNA biogenesis pathways in cancer. Nat Rev Cancer2015; 15: 321–333.
13.
PengYCroceCM.The role of microRNAs in human cancer. Signal Transduct Target Ther2016; 1: 15004.
14.
HirakawaTNasuKAbeW, et alMiR-503, a microRNA epigenetically repressed in endometriosis, induces apoptosis and cell-cycle arrest and inhibits cell proliferation, angiogenesis, and contractility of human ovarian endometriotic stromal cells. Hum Reprod2016; 31: 2587–2597.
15.
AoyagiYNasuKKaiK, et alDecidualization differentially regulates microRNA expression in eutopic and ectopic endometrial stromal cells. Reprod Sci2017; 24: 445–455.
16.
TakebayashiKNasuKOkamotoM, et alhsa-miR-100-5p, an overexpressed miRNA in human ovarian endometriotic stromal cells, promotes invasion through attenuation of SMARCD1 expression. Reprod Biol Endocrinol2020; 18: 31.
17.
ZhuRNasuKHijiyaN, et alHsa-miR-199a-3p inhibits motility, invasiveness, and contractility of ovarian endometriotic stromal cells. Reprod Sci2021; 28: 3498–3507.
18.
NasuKAoyagiYZhuR, et alRole of repressed microRNAs in endometriosis. Med Mol Morphol2022; 55(1): 1–7.
19.
NasuKAoyagiYZhuR, et alPromising therapeutic targets of endometriosis obtained from microRNA studies. Med Mol Morphol2022; 55: 85–90.
20.
MatsumotoHNasuKNishidaM, et alRegulation of proliferation, motility, and contractility of human endometrial stromal cells by platelet-derived growth factor. J Clin Endocrinol Metab2005; 90: 3560–3567.
21.
ShakedIMeersonAWolfY, et alMicroRNA-132 potentiates cholinergic anti-inflammatory signaling by targeting acetylcholinesterase. Immunity2009; 31: 965–973.
22.
MagillSTCambronneXALuikartBW, et almicroRNA-132 regulates dendritic growth and arborization of newborn neurons in the adult hippocampus. Proc Natl Acad Sci USA2010; 107: 20382–20387.
23.
MulikSXuJReddyPB, et alRole of miR-132 in angiogenesis after ocular infection with herpes simplex virus. Am J Pathol2012; 181: 525–534.
24.
LiuQLiaoFWuH, et alUpregulation of miR-132 expression in glioma and its clinical significance. Tumour Biol2014; 35: 12299–12304.
25.
ParkJKHenryJCJiangJ, et almiR-132 and miR-212 are increased in pancreatic cancer and target the retinoblastoma tumor suppressor. Biochem Biophys Res Commun2011; 406: 518–523.
26.
QuWDingSMCaoG, et almiR-132 mediates a metabolic shift in prostate cancer cells by targeting Glut1. FEBS Open Bio2016; 6: 735–741.
27.
ZhangZGChenWXWuYH, et almiR-132 prohibits proliferation, invasion, migration, and metastasis in breast cancer by targeting HN1. Biochem Biophys Res Commun2014; 454: 109–114.
28.
MokutaniYUemuraMMunakataK, et alDown-regulation of microRNA-132 is associated with poor prognosis of colorectal cancer. Ann Surg Oncol2016; 23 Suppl: 599–608.
29.
YouJLiYFangN, et almiR-132 suppresses the migration and invasion of lung cancer cells via targeting the EMT regulator ZEB2. PLoS One2014; 9 :e91827.
30.
LiYZuLWangY, et almiR-132 inhibits lung cancer cell migration and invasion by targeting SOX4. J Thorac Dis2015; 7: 1563–1569.
31.
WangRLiangH.MiR-132, miR-15a and miR-16 synergistically inhibit pituitary tumor cell proliferation, invasion and migration by targeting Sox5. Cancer Lett2015; 356: 568–578.
32.
GengFWuJLLuGF, et alMicroRNA-132 targets PEA-15 and suppresses the progression of astrocytoma in vitro. J Neurooncol2016; 129: 211–220.
33.
TianHHouLXiongYM, et almiR-132 targeting E2F5 suppresses cell proliferation, invasion, migration in ovarian cancer cells. Am J Transl Res2016; 8: 1492–1501.
