Abstract
We are pleased to present this year’s JALA Ten honorees, which highlight significant breakthroughs across the intersection of automation, diagnostics, therapeutics, imaging, manufacturing, and beyond. Examples include new paradigms in design, nucleic acid–based therapeutics, the development of novel nanolithography approaches, and cellular interrogation and control, among others.
Nominations hailed from all corners of the research universe including universities; companies, both early stage and established; and government research laboratories. Honorees have developed new approaches that range from the fundamental to those that have been successfully transitioned into commercial products.
Innovation is a team effort, and this year’s selections demonstrate the remarkable progress that can be realized when top scientists and engineers combine their talents with those of translationally minded clinicians and entrepreneurs toward realizing important advancements that will ultimately improve the quality of health care. It is this same scope that is evident across the membership of the JALA Editorial Board and the principles that guide our publishing philosophy.
The JALA Editorial Board would like to thank all of the enthusiastic nominators for their thoughtful and comprehensive assessments of their respective submissions and salutes the honorees for their continued efforts toward demonstrating the importance of continued support for research and development, enabling innovation to benefit nearly every facet of biology and medicine.
Optimizing Design Outcomes
By Francesco Ciucci1, Tomonori Honda2, and Maria C. Yang2
1Universitat Heidelberg, Germany
2Massachusetts Institute of Technology, Cambridge, MA, USA
One common way to represent a complex engineering system (for example, an aircraft or a desalination plant) is as an overall system that encompasses many subsystems, such as a power subsystem and a structural subsystem. A continuing challenge in the early stages of designing complex engineering systems is making design tradeoffs between subsystems so that the overall system can achieve the best possible performance. One accepted strategy is to apply game theory to a design, treating subsystems as “players” that pass information to each other until the overall system reaches optimality. Current game theoretic approaches pass simple point information and can achieve a Nash equilibrium. This article explores the value of passing additional information by putting it in the form of a quadratic and also a Hessian (compressed into an Eigenvalue), thus making the system more cooperative and therefore lead to highly desirable Pareto optimal solutions. The approach is illustrated through three case examples: (1) a mathematical design problem with two players, (2) an aircraft design problem with two players, and (3) the design of a speed reducer with three players. This article demonstrates that a Pareto solution can be reached in all three of these cases. Using this strategy, designers of complex systems have the potential to achieve more effective design outcomes.
High-Throughput Three-Dimensional Tracking of Human Sperms Using Computational On-Chip Imaging
By Ting-Wei Su1, Liang Xue1, and Aydogan Ozcan1
1University of California, Los Angeles, Los Angeles, CA, USA
This work demonstrates a novel lens-free on-chip imaging technique that can track the three-dimensional (3D) trajectories of >1500 individual human sperms within an observation volume of ~8 to 17 mm3 with submicron accuracy and ~10 to 12 ms temporal resolution. This high-throughput imaging platform achieves more than an order of magnitude larger imaging volume compared with other microscopy tools permitting us to report, for the first time, the helical trajectories of human sperms, an observation that could not be reported before our work, mostly because of tight 3D radii of such helices as well as the rapid rotation speed of human sperms.
Cantilever-Free Scanning Probe Molecular Printing
By Louis Giam1 and Chad A. Mirkin1
1Northwestern University, Evanston, IL, USA
Cantilever-free scanning probe molecular printing techniques (polymer pen lithography and hard-tip, soft-spring lithography) merge the advantages of dip-pen nanolithography and microcontact printing to enable parallel and inexpensive printing of molecular features with nanoscale resolution over large areas. The conceptual advance was the elimination of impractical cantilevers and the use of compliant pyramids mounted on a hard transparent backing or, alternatively, the use of hard tips on a soft-backing layer, also mounted on a transparent substrate, as the molecular delivery vehicles in a piezo-driven scanning probe device. These advances have led to all commercialized forms of molecular printing tools (NanoInk).
Reversible Regulation of Aptamer Activity with Effector-Responsive Hairpin Oligonucleotides
By Na Li1
1University of Miami, Miami, FL, USA
Most of the existing strategies for regulating aptamer activity have a limited specificity and/or reversibility. This work demonstrated a simple, generic strategy to simultaneously achieve specificity and reversibility by exploiting the spontaneous conformational change of hairpin oligonucleotides upon the specific recognition of nucleic acid effectors. This new strategy has been demonstrated with an anticoagulant aptamer. With further optimization and development, this strategy could potentially be used to create on-demand aptamer therapy. The potency of the therapy (the aptamer activity) is continuously adjusted based on the disease status that is indicated by the amount of effectors (mRNA or DNA biomarkers).
Hydrodynamic Stretching of Single Cells for Large Population Mechanical Phenotyping
By Daniel R. Gossett1, Henry T. K. Tse1, Serena A. Lee1, Yong Ying1, Anne G. Lindgren1, Otto O. Yang1, Jianyu Rao1, Amander T. Clark1, and Dino Di Carlo1
1University of California, Los Angeles, Los Angeles, CA, USA
Biomarkers for cell type, state, and function enable critical classifications in biological research and medical diagnostics. Recently, biophysical markers have been shown to be attractive, label-free alternatives to conventional biochemical biomarkers. The potential impact of these biophysical markers spans many areas of biomedicine including regenerative medicine, clinical cancer diagnostics, and immune monitoring. However, current methods of probing single-cell mechanical properties are either too labor intensive for clinical adoption or too low in throughput to accurately sample the heterogeneity of biological samples. Dino Di Carlo and his research group have recently developed a technique called deformability cytometry, which couples microfluidic hydrodynamic stretching with high-speed imaging and automated image analysis to probe single-cell deformability at a rate of more than 1000 cells/s. This throughput enables thorough sampling of clinically relevant complex and heterogeneous biological fluids (e.g., blood, pleural effusions, and urine). The limited operational complexity and minimal sample preparation requirements of this method will ease translation as both a research tool and clinical diagnostic tool. Deformability cytometry makes mechanical phenotyping robust and accessible to biomedicine.
Thermal Biosensing with Phase Change Nanoparticles
By Chaoming Wang1, Zhaoyong Sun1, Liyuan Ma1, and Ming Su7
1University of Central Florida, Orlando, FL, USA
Nanoparticles of optical, magnetic, and electric character can detect biomarkers with high sensitivities but with low multiplicity due to low spectral resolution or nondistinguishable particle property. A panel of nanoparticles of phase-change materials (nano-PCM) has been used to detect multiple biomarkers with differential scanning calorimetry. The nanoparticles are made of either pure metals or eutectic alloys and have sharp and distinct melting peaks during linear temperature rise. After forming one-to-one accordance between each type of nanoparticle and ligand (i.e., antibody or DNA), multiple molecular variations can be detected at the same time for efficient drug discovery and medical diagnosis.
Controlling Spatial Organization of Multiple Cell Types in Defined 3D Geometries
By Halil Tekin1,2, Jefferson G. Sanchez1,2, Christian Landeros1,2, Karen Dubbin1,2, Robert Langer1 and Ali Khademhosseini1,2
1Massachusetts Institute of Technology, Cambridge, MA, USA
2Harvard Medical School, Boston, MA, USA
Multicellular communities are structurally and functionally complex. Various spatially distributed cell types in defined microenvironments produce structural complexity. Functional complexity results from the intricate interactions between multiple cell types, which regulate native tissue functions, such as specific organ functions, cancer dynamics, and developmental stages. Recreating these complexities in vitro would be highly useful to fabricate particular tissue constructs for regenerative medicine, create tumor models for drug discovery, and form biomimetic microenvironments to study developmental biology. However, it has remained a challenge to obtain targeted spatial organization of various cell types in defined microenvironments. Herein, we introduce poly(N-isopropylacrylamide; PNIPAAm)–based dynamic microwells to spatially arrange multiple cell types in defined 3D geometries by exploiting the shape change properties of microwells at different temperatures. Two biologically relevant different cell types were spatially distributed in square and circular geometries by seeding them at different temperatures. This work is versatile and could potentially be useful for applications in tissue engineering, cancer biology, developmental biology, and drug discovery.
Selective Trapping and Manipulation of Microscale Objects Using Mobile Microvortices
By Li Zhang1,2, Tristan Petit1,3, Kathrin E. Peyer1, Bradley Kratochvil1, and Bradley J. Nelson1
1Institute of Robotics and Intelligent Systems, Zurich, Switzerland
2The Chinese University of Hong Kong, Hong Kong, China
3Diamond Sensors Laboratory, Gif-sur-Yvette, France
This work describes a new technology to trap and manipulate microscale objects, such as microparticles and Escherichia coli bacteria, using mobile microvortices generated by a rotating nanowire or self-assembled microspheres. Unlike conventional microfluidic devices, the mobile microvortex performs selective manipulation of individual micro-objects with micrometer positioning precision without the need for fabricating additional features on the manipulation surface. Furthermore, the mobile microvortices, with volumes down to femto-liters, provide noncontact, minimally invasive manipulation of cells and other biological samples in environments almost identical to their normal physiological conditions. Another primary advantage of mobile-microvortex–based manipulation is that there is no specific requirement on the material properties of the micro-object.
Acoustic Tweezers: A Noninvasive, Noncontact, Versatile, On-Chip Platform for Cell Manipulation
By Xiaoyun Ding1, Sz-Chin Steven Lin1, Brian Kiraly1, Hongjun Yue1, Sixing Li1, I-Kao Chiang1, Jinjie Shi1, Stephen J. Benkovic1, and Tony Jun Huang1
1The Pennsylvania State University, University Park, PA, USA
Professor Tony Jun Huang’s research group at The Pennsylvania State University pioneered the first surface acoustic wave-based manipulation platform, so-called “acoustic tweezers,” which can trap and dexterously manipulate single microparticles, cells, and entire organisms (i.e., Caenorhabditis elegans) along a programmed route in two dimensions within a dime-sized microfluidic chip. The acoustic tweezers can move a 10 µm single polystyrene bead to write the word PNAS (fig. 1A), a bovine red blood cell to trace the letters PSU (fig. 1B), and a single C. elegans in an x-y plane (fig. 1C). It was also the first technology capable of touchless trapping and manipulating C. elegans, a 1-mm-long roundworm that is an important model system for studying diseases and development in humans. With its advantages in noninvasiveness, miniaturization, and versatility, our acoustic tweezers will become a powerful tool for many disciplines of science and engineering.
Reprinted with permission from Ciucci F, Honda T, Yang MC. An Information Passing Strategy for Achieving Pareto Optimality in the Design of Complex Systems. Res. Eng. Des. 2013, 23(1), 71–83.
Reprinted with permission from Su T-W, Xue L, Ozcan A. High-Throughput Lensfree 3D Tracking of Human Sperms Reveals Rare Statistics of Helical Trajectories. Proc. Natl. Acad. Sci. U. S. A. 2012. DOI: 10.1073/pnas.1212506109
Reprinted with permission from Giam L, Mirkin C. Cantilever-Free Scanning Probe Molecular Printing. Angew. Chem. Intl. Ed. 2011, 33, 7482–7485.
Reprinted with permission from Li N. Reversible Regulation of Aptamer Activity with Effector-Responsive Hairpin Oligonucleotides. J. Lab Autom. 2012. DOI: 10.1177/2211068212448429
Reprinted with permission from Gossett Daniel R, et al. Hydrodynamic Stretching of Single Cells for Large Population Mechanical Phenotyping. Proc. Natl. Acad. Sci. U. S. A. 2012, 109, 7630–7635.
Reprinted with permission from Wang C, et al. Simultaneous Detection of Multiple Biomarkers with Several Orders of Concentration Difference Using Phase Change Nanoparticles. Anal. Chem. 2011, 83, 2215.
Reprinted with permission from Tekin H, et al. Controlling Spatial Organization of Multiple Cell Types in Defined 3D Geometries. Adv. Mater. 2012. DOI: 10.1002/adma.201201805
Reprinted with permission from Petit T, Zhang L, Peyer KE, Kratochvil B, Nelson BJ. Selective Trapping and Manipulation of Microscale Objects Using Mobile Microvortices. Nano Lett. 2012, 12, 156–160.
Reprinted with permission from Ding et al. On-Chip Manipulation of Single Microparticles, Cells, and Organisms Using Surface Acoustic Waves. Proc. Natl. Acad. Sci. U. S. A. 2012, 109, 11105–11109.
Reprinted with permission from Tewson P, Westenberg M, Zhao Y, Campbell R, Quinn A, Hughes T. Simultaneous Detection of Ca2+ and Diacylglycerol Signaling in Living Cells. PLoS ONE 2012, 7, e42791.
Simultaneous Detection of Ca2+ and Diacylglycerol Signaling in Living Cells
By Paul Tewson1, Mara Westenberg1, Yongxin Zhao2, Robert E. Campbell3, Anne Marie Quinn1 and Thomas E. Hughes1,3
1Montana Molecular, Bozeman, MT, USA
2University of Alberta, Edmonton, Alberta, Canada
3Montana State University, Bozeman, MT, USA
Cell-based calcium assays have been a mainstay of drug discovery and research since the 1990s. However, Ca2+ is one component of a complex network of interacting pathways that signal via multiple second messengers. Researchers in Montana created a green fluorescent sensor for diacylglycerol and paired it with a red fluorescent Ca2+ sensor, producing a robust, no-wash, multiplex assay that simultaneously detects two second messengers of GPCR signaling. This work demonstrates an approach to producing multiplex assays with improved specificity and more information content. Such assays are suitable for microscopy and use on multimode fluorescence plate readers.
Footnotes
All figures are reprinted with permission from the copyright holder.