Abstract
A method is presented for the computer synthesis of deoxyribonucleic acid (DNA) histograms, based on estimates of cell distribution around a life cycle, DNA synthesis rate and instrumentally introduced dispersion. Synthetic histograms are compared with experimental DNA histograms for two cell systems; in one, the cell population is in asynchronous exponential growth (steady state), in the other, the cell population is recovering from a block at the G1/S phase interface (nonsteady state). The average phase transit times and the associated dispersions are determined, using a DNA histogram synthesis technique, for the nonsteady state population.