Oocytes collected from patients following ovarian stimulation exhibit varying qualities. The quality of oocytes has an effect on its subsequent fertilization, developmental competence post-fertilization and the viability of resultant embryos. The aim of this article was to review the morphological criteria devised for assessment of oocyte quality by ordinary light and polarized light microscopy before IVF or ICSI. The parameters employed in the evaluation of oocyte morphology include the appearance of: structure of the cumulus–oocyte complex, oocyte cytoplasm, perivitelline space, zona pellucida, polar body and meiotic spindle.
AliJJoshiHNAl-BadrMShahataMAMAbdulkaderAAl-NatshaSFlamerziMHamshoA. (1998) Subzonal insemination of immature oocytes generated during intracytoplasmic injection cycles. Med. Sci. Res. (UK)26:389–391.
2.
AnifandisGDafopoulosKMessiniCIChalvatzasNMessinisIE. Effect of the position of the polar body during ICSI on fertilization rate and embryo development. Reprod Sci.2010; 17(9): 849–853.
3.
BalabanBUrmanBSertacAAlatasCAksoySMercanR. Oocyte morphology does not affect fertilization rate, embryo quality and implantation rate after intracytoplasmic sperm injection. Hum Reprod. 1998; 13(12): 3431–3433.
4.
BarnesFLFirstNL. Embryonic transcription in in vitro cultured bovine embryos. Mol Reprod Dev.1991; 29:117–123.
5.
BattagliaDEGoodwinPKleinNASoulesMR. Infuence of maternal age on meiotic spindle in oocytes from naturally cycling women. Hum Reprod. 1996; 11:2217–2222.
6.
BertrandEVan den BerghMEnglertY. Does zona pellucida thickness influence the fertilization rate?Hum Reprod. 1995; 10(5): 1189–1193.
7.
BonettiACerviMTomeiFMarchiniMOrtolaniFMannoM. Ultrastructural evaluation of human metaphase II oocytes after vitrification: closed versus open devices. Fertil Steril. 2011; 95(3): 928–935.
CavillaJLKennedyCRByskovAGHartshorneGM. Human immature oocytes grow during culture for IVM. Hum Reprod. 2008; 23(1): 37–45.
10.
CiottiPMNotarangeloLMorselli-LabateAMFellettiVPorcuEVenturoliS. First polar body morphology before ICSI is not related to embryo quality or pregnancy rate. Hum Reprod. 2004; 19(10): 2334–2339.
11.
CombellesCMRacowskyC. Assessment and optimization of oocyte quality during assisted reproductive technology treatment. Semin Reprod Med.2005; 23:277–284.
12.
CookeSTylerJPDriscollGL. Meiotic spindle location and identification and its effect on embryonic cleavage plane and early development. Hum Reprod. 2003; 18:2397–2405.
13.
CoticchioGBoriniADistratisVMaioneMScaravelliGBianchiVMacchiarelliGNottolaSA. Qualitative and morphometric analysis of the ultrastructure of human oocytes cryopreserved by two alternative slow cooling protocols. J Assist Reprod Genet. 2010a; 27:131–140.
14.
CoticchioGSciajnoRHuttKBromfieldJBoriniAAlbertiniDF. Comparative analysis of the metaphase II spindle of human oocytes through polarized light and high-performance confocal microscopy. Fertil Steril. 2010b; 93(6): 2056–2064.
15.
CoticchioGSereniESerraoLMazzoneSIadarolaIBoriniA. What criteria for the definition of oocyte quality?Ann NY Acad Sci.2004; 1034:132–144.
16.
De SousaPACaveneyAWesthusinMEWatsonAJ. Temporal patterns of embryonic gene expression and their dependence on 11 oogenetic factors. Theriogenol. 1998; 49:115–128.
17.
De SutterPDozortsevDQianCDhontM. Oocyte morphology does not correlate with fertilization rate and embryo quality after intracytoplasmic sperm injection. Hum Reprod. 1996; 11:595–597.
18.
DeVosAVan de VeldeHJorisHVan SteirteghemAC. In-vitro matured metaphase-I oocytes have a lower fertilization rate but similar embryo quality as mature metaphase-II oocytes after intracytoplasmic sperm injection. Hum Reprod1999; 14:1859–1863.
19.
DurinziKLSanigaEMLanzendorfSE. The relationship between size and maturation in vitro in the unstimulated human oocyte. Fertil Steril. 1995; 63:404–406.
20.
EbnerTMoserMSommergruberCYamanCPflegerUTewsG. First polar body morphology and blastocyst formation rate in ICSI patients. Hum Reprod. 2002; 17:2415–2418.
21.
EbnerTMoserMSommergruberMTewsG. Selection based on morphological assessment of oocytes and embryos at different stages of preimplantation development: a review. Hum Reprod Update. 2003; 9:251–262.
EbnerTYamanCMoserMSommergruberMFeichtingerOTewsG. Prognostic value of first polar body morphology on fertilization rate and embryo quality in intracytoplasmic sperm injection. Hum Reprod. 2000; 15:427–430.
25.
Eichenlaub-RitterUSchmiadyHKentenichHSoewartoD. Recurrent failure in polar body formation and premature chromosome condensation in oocytes from a human patient: indicators of asynchrony in nuclear and cytoplasmic maturation. Hum Reprod. 1995; 10:2343–2349.
26.
FanHYSunQY. Involvement of mitogen activated protein kinase cascade during oocyte maturation and fertilization in mammals. Biol Reprod2004; 70:535–547.
27.
FangCTangMLiTPengWLZhouCQZhuangGLLeongM. Visualization of meiotic spindle and subsequent embryonic development in in vitro and in vivo matured human oocytes. J Assist Reprod Genet. 2007; 24(11): 495–499.
28.
GabrielsenALindenbergSPetersenK. The impact of the zona pellucida thickness variation of human embryos on pregnancy outcome in relation to suboptimal embryo development. A prospective randomized controlled study. Hum Reprod. 2001; 16:2166–2170.
29.
GaoSLiYGaoXHuJYangHChenZJ. Spindle and Chromosome Changes of Human MII Oocytes During Incubation After Slow Freezing/Fast Thawing Procedures. Reproductive Sciences2009; 16(4): 391–396.
30.
Hassan-AliHHisham-SalehAEl-GezeiryDBaghdadyIIsmaeilIMandelbaumJ. Perivitelline space granularity: a sign of human menopausal gonadotrophin overdose in intracytoplasmic sperm injection. Hum Reprod. 1998; 13:3425–3430.
31.
HeindryckxBDe GheselleSLiermanSGerrisJDe SutterP. Efficiency of polarized microscopy as a predictive tool for human oocyte quality. Hum Reprod. 2011; 26(3): 535–544.
32.
KahramanSYakinKDonmezESamliHBahceMCengizGSertyelSSamliMImirzaliogluN. Relationship between granular cytoplasm of oocytes and pregnancy outcome following intracytoplasmic sperm injection. Hum Reprod. 2000; 15:2390–2393.
33.
KastropPMBeversMMDestreeOHKruipTA. Protein synthesis and phosphorylation patterns of bovine oocytes maturing in vivo. Mol Reprod Dev.1991; 29:271–275.
34.
KhaliliMAMojibianMSultanAM. Role of oocyte morphology on fertilization and embryo formation in assisted reproductive techniques. Middle East Fertil Soc J.2005; 10(1): 72–77.
35.
KilaniSCookeSChapmanM. Time course of meiotic spindle development in MII oocytes. Zygote. 2011; 19(1): 55–62.
36.
KorkmazCCinarOAkyolM. The relationship between meiotic spindle imaging and outcome of intracytoplasmic sperm injection: a retrospective study. Gynecol Endocrinol. 2011; [Epub ahead of print]
37.
KrisherRL. The effect of oocyte quality on development. J Anim Sci2004; 82:E14–E23.
38.
LoutradisDDrakakisPKallianidisKMilingosSDendrinosSMichalasS. Oocyte morphology correlates with embryo quality and pregnancy rate after intracytoplasmic sperm injection. Fertil Steril. 1999; 72:240–244.
MiaoYLKikuchiKSunQYSchattenH. Oocyte aging: cellular and molecular changes, developmental potential and reversal possibility. Hum Reprod Update. 2009; 15(5): 573–585.
41.
MoonJHHyunCSLeeSWSonWYYoonSHLimJH. Visualization of the metaphase II meiotic spindle in living human oocytes using the Polscope enables the prediction of embryonic developmental competence after ICSI. Hum Reprod. 2003; 18:817–820.
42.
NavarroPAde AraújoMMde AraújoCMRochaMdos ReisRMartinsW. Relationship between first polar body morphology before intracytoplasmic sperm injection and fertilization rate, cleavage rate, and embryo quality. Int J Gyn Obstet. 2009; 104(3): 226–229.
43.
OtsukiJNagaiYChibaK. Lipofuscin bodies in human oocytes as an indicator of oocyte quality. J Assist Reprod Genet. 2007; 24(7): 263–270.
44.
OtsukiJ. Intracytoplasmic morphological abnormalities in human oocytes. J Mamm Ova Res.2009; 26:26–31.
45.
PelletierCKeefeDLTrimarchiJR. Noninvasive polarized light microscopy quantitatively distinguishes the multilaminar structure of the zona pellucida of living human eggs and embryos. Fertil Steril. 2004; 81(Suppl 1): 850–856.
46.
PetersenCGOliveiraJBMauriALMassaroFCBaruffiRLPontesAFrancoJGJr. Relationship between visualization of meiotic spindle in human oocytes and ICSI outcomes: a meta-analysis. Reprod Biomed Online. 2009; 18(2): 235–243.
47.
RienziLMartinezFIacobelliMMinasiMGFerreroSTesarikJGrecoE. Relationship between meiotic spindle location with regard to the polar body position and oocyte developmental potential after ICSI. Hum Reprod. 2003; 18:1289–1293.
48.
RienziLUbaldiFMIacobelliMMinasiMGRomanoSFerreroSSapienzaFBaroniELitwickaKGrecoE. Significance of metaphase II human oocyte morphology on ICSI outcome. Fertil Steril. 2008; 90(5): 1692–700.
49.
RienziLVajtaGUbaldiF. Predictive value of oocyte morphology in human IVF: a systematic review of the literature. Hum Reprod Update. 2011; 17(1): 34–45.
ShenYStalfTMehnertCDe SantisLCinoITinnebergHREichenlaub-RitterU. Light retardance by human oocyte spindle is positively related to pronuclear score after ICSI. Reprod Biomed Online. 2006; 12(6): 737–751.
52.
SilvaCPKommineniKOldenbourgRKeefeDL. The first polar body does not predict accurately the location of the metaphase 2 meiotic spindle in mammalian oocytes. Fertil Steril. 1999; 71:719–721.
53.
SirardMAFlormanHMLeibfried-RutledgeMLBarnesFLSimsMLFirstNL. Timing of nuclear progression and protein synthesis necessary for meiotic maturation of bovine oocytes. Biol Reprod. 1989; 40:1257–1263.
54.
SirardMARichardFBlondinPRobertC. Contribution of the oocyte to embryo quality. Theriogenol. 2006; 65:126–136.
55.
StrassburgerDFriedlerSRazielAKastersteinESchachterMRon-ElR. The outcome of ICSI of immature MI oocytes and rescued in vitro matured MII oocytes. Hum Reprod2004; 19(7): 1587–1590.
SunQYWuGMLaiLParkKWCabotRCheongHTDayBNPratherRSSchattenH. Translocation of active mitochondria during pig oocyte maturation, fertilization and early embryo development in vitro. Reprod. 2001; 122:155–163.
58.
TrimarchiJRKeefeDL. Assessing the quality of oocytes derived from in vitro maturation: are we looking under the lamppost?Fertil Steril. 2006; 85:839–840.
59.
TriwitayakornASuwajanakornSPruksananondaKSereepapongWAhnonkitpanitV. Correlation Between Human Follicular Diameter and Oocyte Outcomes in an ICSI Program. J of Assist Reprod Genet. 2003; 20(4): 143–147.
60.
UbaldiFRienziL. Morphological Selection of Gametes. Placenta. 2008; 29:S115–S120.
61.
VassenaRMapletoftRJAllodiSSinghJAdamsGP. Morphology and developmental competence of bovine oocytes relative to follicular status. Theriogenology2003; 60:923–932.
62.
VeeckL. Abnormal morphology of the human oocyte and conceptus. In: An Atlas of Human Gametes and Conceptuses. Lancs, UK: The Parthenon. Publishing Group Ltd, 1999.
63.
VieiraRCBarcelosIDFerreiraEMMartinsWPFerrianiRANavarroPA. Spindle and chromosome configurations of in vitro-matured oocytes from polycystic ovary syndrome and ovulatory infertile women: a pilot study. J Assist Reprod Genet. 2011; 28(1): 15–21.
64.
WallbuttonSKasraieJ. Vacuolated oocytes: fertilization and embryonic arrest following intra-cytoplasmic sperm injection in a patient exhibiting persistent oocyte macro vacuolization–case report. J Assist Reprod Genet. 2010; 27(4): 183–188.
65.
WangQSunQY. Evaluation of oocyte quality: morphological, cellular and molecular predictors. Reprod Fertil Dev.2007; 19:1–12.
66.
WangWHKeefeDL. Spindle observation in living mammalian oocytes with the polarization microscope and its practical use. Cloning Stem Cells. 2002; (4): 269–276.
67.
WangWHMengLHackettRJKeefeDL. Developmental ability of human oocytes with or without birefringent spindles imaged by Polscope before insemination. Hum Reprod. 2001a; 16:1464–1468.
68.
WangWHMengLHackettRJOdenbourgRKeefeDL. The spindle observation and its relationship with fertilization after intracytoplasmic sperm injection in living human oocytes. Fertil Steril. 2001b; 75:348–353.
69.
XiaP. Intracytoplasmic sperm injection: correlation of oocyte grade based on polar body, perivitelline space and cytoplasmic inclusions with fertilization rate and embryo quality. Hum Reprod. 1997; 12:1750–1755.
70.
XuMBarrettSLWest-FarrellEKondapalliLAKiesewetterSESheaLDWoodruffTK. In vitro grown human ovarian follicles from cancer patients support oocyte growth. Hum Reprod. 2009; 24(10): 2531–2540.
71.
YounisJSRadinOIzhakiIBen-AmiM. Does first polar body morphology predict oocyte performance during ICSI treatment?J Assist Reprod Genet. 2009; 26(11–12): 561–567.
72.
YounisJSRadinOIzhakiIBen-AmiM. Does first polar body morphology predict oocyte performance during ICSI treatment?J Assist Reprod Genet. 2009; 26(11–12): 561–567.
73.
YuYMaiQChenXWangLGaoLZhouCZhouQ. Assessment of the developmental competence of human somatic cell nuclear transfer embryos by oocyte morphology classification. Hum Reprod. 2009; 24(3): 649–657.
