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Abstract

Ageratina adenophora is an invasive weed with widespread distribution. During the last several decades, many biologically active secondary metabolites have been isolated and characterized from A. adenophora, some of them having inspired the research and development of new therapeutic agents. This review mainly focuses on biological properties of A. adenophora, including the toxicity, antibacterial, antifungal, insecticidal, antiviral activities and others. In addition, the current limits and potentials of A. adenophora and its extracts are also discussed.

Keywords

active compounds phamaceutical effects application

Introduction

Ageratina adenophora, also known as Eupatorium adenophorum, belongs to the genus Agerina in the Compositae family including multiple variants, such as E. adenophorum var. adenophorum, E. adenophorum var. peruvianum Hieron, Eupatorium glandulosum Michx, E. glandulosum Hort. ex Kunth [Illegitimate] and Eupatorium pasadenense Parish. A. adenophora is a perennial semi-herbaceous plant, originating in Mexico and Costa Rica, and has successfully invaded Europe, Oceania and Asia.¹ In 1940, Eupatorium adenophorum invaded Yunnan Province of China from the China-Myanmar border and spread widely to Sichuan, Guangxi, Guizhou, Hubei and Tibet provinces, as well as Chongqing and even Taiwan.² As one of the most important invasive plants in China, A. adenophora affects more than 30 million hectares in China.³ It is estimated that A. adenophora will spread further north and east at an average speed of 20 kilometers per year.² However, there are no effective methods available at present to prevent and control its spread.

A. adenophora is rich in bioactive compounds, including monoterpenes, sesquiterpenes, diterpenes, triterpenes, flavonoids, polysaccharides, pyrrolizidine alkaloids, essential oils, phenylpropanoids etc., of which terpenes are the predominant component.⁴ Those compounds from A. adenophora exhibit a wide range of biological activities, playing an important role in discovering clues to drug development for human disease treatment. For example, in India, A. adenophora, as a common folk medicine, has the property of antibacterial, sterilization, coagulation, analgesia and antipyretic.⁵ In this review, we have mainly discussed the current research on A. adenophora extracts and their biological activities (Table 1).

Table 1.

Biological properties of Ageratina adenophora and its extracts.

No.	Activity	Source or Compound Name	Effect and Application	References
1	Toxicity	Whole grass Methanol extract DAOA, OA and Euptox A	– Respiratory disease in horses. – Extensive inflammation on mice. – Toxicity of liver, spleen and kidney in goat. – Hepatotoxicity, jaundice to mice and rat. – Immuno-toxic and hepatotoxicity to mice.	[6–15]
2	Antibacterial effects	Oil extract Organic and aqueous extracts Euptox A Thymol derivatives	– Against Erwinia herbicola and Pseudomonas putida from plants. – Inhibition of the growth of Bacillus subtilis, Bacillus cereus, Staphylococcus aureus, Escherichia coli, Klebsiella aerogenes and Pseudomonas aeruginosa. – Against the Ralstonia solanacearum – Against both Gram-negative and Gram-positive bacteria	[16–21]
3	Antifungal effects	Leaf extracts Euptox A Oil extract	– Inhibited the formation of Pythium myriotylum mycelial. – Against Fusarium oxysporum, Bipolaris sorokiniana, Fusarium proliferatum and Alternaria tenuissima as well as spore production in F. oxysporum and B. sorokiniana. – Against Phytophthora capsici.	[5,19,22–25]
4	Antiviral effects	Leaf extract Euptox A	– Inhibition of tobacco mosaic virus infection by disaggregating virus particles. Inhibition of H5N1 subtype of influenza virus and alleviate the flu symptoms by enhancing anti-viral immune response. – Inhibition Newcastle Disease Virus infection of chicken embryo fibroblasts.	[26–29]
5	Anti-nematode and insecticidal effects	Aqueous extract Acetone extract Ethanol extract and Euptox A 5,6-dihydroxycadinan -3-ene-2,7-dione	– Against Oncomelania hupensis in humans and livestock. – Against coccidian oocysts in chickens and Aphis gossypii. – Against cabbage aphids and Brevicoryne brassicae. – Against Psoroptes cuniculi and Sarcoptes scabiei in rabbit. – Against Meloidogyne incognita.	[30–40]
6	Other effects	Sesquiterpene Whole grass	– Antitumor effects and inhibition of cancer cell proliferation. – Animal feed after detoxification. – - Inhibit the inflammatory reaction	[41–43]

Toxicity

A. adenophora is highly toxic to animals and affects many organs of animals. Studies have shown that horses feeding on A. adenophora will develop respiratory diseases.⁶ Multifocal parenchymal necrosis and liver degeneration can be caused by intragastric injection of freeze-dried A. adenophora leaf powder or methanol extract in mice.⁷ In another study, rats fed with 25% (w/w) freeze-dried A. adenophora powder will induce jaundice, mainly manifested by increased plasma bilirubin, ALP, ALT and AST levels.⁸ Furthermore, studies have proved that A. adenophora has toxic effects on the liver, spleen and kidney by inducing autophagy and apoptosis of cells in goats and mice, respectively.⁹ In addition, after feeding A. adenophora with a dose of more than 20%, the liver index of mice increased and caused extensive inflammation, in addition to the decrease of antioxidant activity, increasing the production of reactive oxygen species, and activating pyroptosis via the NLRP3-dependent pathway. This indicated that A. adenophora-induced liver damage was mediated via increased oxidative stress.¹⁰

The structural and functional characteristics of the liver determine its increased susceptibility to pathogens and toxins.¹¹ The major toxin isolated from A. adenophora is 9-oxo-10, 11-dehydro-agerophorone (Euptox A), one of the twenty sesquiterpenes with the same molecular skeleton of cadinene present in the weed. Euptox A is a major hepatotoxin for rodents, with a median lethal dose (LD50) of 1470 mg/kg body weight in mice.¹² Two more toxic sesquiterpenes isolated from A. adenophora—2-deoxo-2-(acetyloxy)-9-oxo-ageraphorone (DAOA) and 9-oxo-agerophorone (OA)—are also hepatotoxic in mice with respective LD₅₀ 926 mg/kg BW and 1470 mg/kg BW.¹³ DAOA and Euptox A are also immuno-toxic to mice, leading to decreased numbers and irregular arrangement of splenocytes and thymocytes. Consistent with this, we found that Euptox A arrested splenocyte proliferation in G0/G1 phage and induced autophagy in a dose-dependent manner when administered to mice via the gastric route.¹⁴ In addition, three sesquiterpenes (amorpha-4, 7 (11)—diene-8—a DTD), eupatoranone and 9-oxo-radinone) and two esters, dibutyl phthalate and di (2-ethylhexyl) phthalate (DEHP), were purified from the root of A. adenophora as phytotoxic compounds and played a key role in allelopathy.¹⁵

Antibacterial effects

In recent decades, the emergence of drug-resistant strains has prompted researchers to pay more and more attention to the development of novel antibacterial agents. Plant extracts are potentially rich sources of antimicrobial compounds, and are considered clinically and ecologically safer compared to synthetic chemicals.¹⁶ In Vietnam, A. adenophora is used to treat various skin infections, and its oil-extract has obvious inhibitory effect on Erwinia herbicola and Pseudomonas putida, two phyto-pathogenic bacteria.¹⁷ The organic phase and water extracts from the crude leaves of A. adenophora have inhibitory effects on the growth of Bacillus subtilis, Bacillus cereus, Staphylococcus aureus, Escherichia coli, Klebsiella aerogenes and Pseudomonas aeruginosa.¹⁸ In addition, Euptox A has a strong inhibitory effect on Ralstonia solanacearum (R1-4), which is widely distributed in plant pathogens, with a minimum inhibitor of 0.25 to 1 mg/ml, while OA has moderate toxicity to animals.¹⁹ The thymol derivatives of A. adenophora have also exhibited inhibitory effects on both gram-negative and gram-positive bacteria.²⁰ Furthermore, bioactive quinic acid derivatives from A. adenophora have in vitro antibacterial activity toward five assayed bacterial strains.²¹

Antifungal effects

Secondary metabolites refer to compounds that are synthesized under stress conditions, and primarily inhibit pathogens. A number of secondary metabolites, mainly sesquiterpenes, have been isolated from the inflorescence and roots of A. adenophora that show potent antifungal activity. A study shows that the crude extract of A. adenophora has inhibitory effect on pathogenic fungi.²² A later research demonstrated that sesquiterpenoids, the main active component of A. adenophora, mainly isolated from inflorescence and root.⁵ In addition, Liu et al.²³ reported that the leaf extracts of A. adenophora (mainly 10Hβ-9-oxo-agerophorone, 10Hα-9-oxo-agerophorone and Euptox A) inhibited the formation of Pythium myriotylum mycelial biomass at the minimum inhibitory concentration of 100 μg/ml. Euptox A also inhibited germination of Fusarium oxysporum, Bipolaris sorokiniana, Fusarium proliferatum and Alternaria tenuissima as well as spore production in F. oxysporum and B. sorokiniana.¹⁹ Furthermore, a bioactive quinic acid derivatives from A. adenophora has in vitro anti-fungal activity against spore germination of Magnaporthe grisea with an IC₅₀ value 542.3 µM.²¹ Six compounds isolated from the root of A. adenophora have antifungal activity against Alternaria alternata and four other agricultural pathogenic fungi Colletotrichum gloesporioides, C.musae, Rhzoctonia solani and Fusarium oxysporum f.sp.niveum.²⁴ Finally, the mycelial growth of Phytophthora capsici was inhibited by A. adenophora oil extraction after incubation 7 d at the concentration of 500 μg/mL.²⁵

Antiviral effects

Pathogenic viruses affect humans, farm animals and plants, resulting in considerable mortality, morbidity and economic losses worldwide.²⁶ Furthermore, the frequent emergence of new vaccine-resistant mutant strains render most vaccination and treatment regimens ineffectual. Herbal formulations have been used since millennia to prevent and treat viral infections and enhance immunity, and much of the current research on developing antiviral drugs is focused on medicinal plants. A. adenophora inhibits the growth of other plants via allelopathy, indicating its potential as an anti-viral agent as well.²⁷ For example, leaf extract from A. adenophora inhibited tobacco mosaic virus infection by disaggregating virus particles, as observed under by electron microscopy.²⁷ In addition to plant virus, A. adenophora is also effective against animal virus. In addition, the polysaccharides in A. adenophora leaf extracts inhibited the highly pathogenic H5N1 subtype of influenza virus, and alleviated the flu symptoms by enhancing anti-viral immune response via IL-6, TNF-α and IFN-γ.²⁸ Xu et al.²⁹ reported that Euptox A extracted from A. adenophora neutralizes the Newcastle disease virus, then inhibited the infection of chicken embryo fibroblasts.

Anti-nematode and insecticidal effects

In recent years, the potential of A. adenophora as anti-nematode agent and insecticide has been gradually recognized, and its extract has been approved for clinical application in Vietnam.³⁰ Aqueous extracts of A. adenophora inhibited Oncomelania hupensis and controlled schistosomiasis spread in humans and livestock.³¹ It is also significantly effective against coccidian oocysts and Aphis gossypii.³² In addition, the A. adenophora acetone extraction has a strong inactivation effects against cabbage aphids and Brevicoryne brassicae.³³ Acariasis is a highly infectious skin disease caused by mites, which frequently affects livestock and can lead to decreased productivity and even death.³⁴ Although several chemical acaricidic drugs are available to treat and control ectoparasitic acariasis,³⁵ their therapeutic utility is limited by bioaccumulation and the emergence of resistant strains. In contrast, botanical acaricides are biodegradable and do not have the risk of resistance.³⁶ The ethanol extract of A. adenophora showed potent toxicity against the mites Psoroptes cuniculi and Sarcoptes scabiei in a time and concentration-dependent manner,⁴⁰ and its crude extract has shown satisfactory clinical outcome on rabbits with spontaneous and induced mite infection.³² Euptox A, was recently characterized by silica gel column chromatography, thin-layer chromatography and ¹H, ¹³C nuclear magnetic resonance, and makes up a considerable proportion of A. adenophora-derived toxins.³⁷ It showed potent toxic effects against S. scabiei and P. cuniculi.³⁸ Four cadinene sesquiterpenes were isolated from A. adenophora leaves with ethyl acetate and identified by gas chromatography-mass spectrometry, of which 5,6-dihydroxycadinan-3-ene-2,7-dione exhibited significant antinemic activity against Meloidogyne incognita.³⁹

Others

It is reported that the extract and secondary metabolites of A. adenophora have anti-inflammatory activity. For example, the ethanol leaf extract of A. adenophora shows anti-inflammatory activity by inhibiting gene expression of IL-1β and cyclooxygenase 2 (COX-2).⁴¹ In addition, the ethanol leaf extract of A. adenophora can effectively inhibit the inflammatory reaction of foot paw caused by injection of DNFB. Furthermore, intravenous injection of A. adenophora leaf extract can increase the number of CD4⁺T cells in spleen and induce TGFβ gene coding and inhibiting IL1β And COX-2 expression.⁴²

The study also found that the extract of A. adenophora has anti-tumor activity. In vitro tests showed that the inhibition rate of 9-oxo10,11-dehydroezelanone isolated from A. adenophora on human lung cancer A549 cells, Hela cells and Hep-2 cells were 76.42%, 68.30% and 79.05% at a dose of 500 μg/mL, respectively.⁴³

Summary and future perspectives

Due to its easy absorption, the liver is one of the main target organs of A. adenophora toxins, especially sesquiterpenoids.¹¹ In addition, the terpenoids and terpened that have not been isolated so far could exert hepatotoxic effects. A. adenophora or its extracts induce liver toxicity by activating oxidative stress, pytoptosis,¹⁰ and atoptosis,⁹ although the exact mechanism, as well as the metabolic effects of A. adenophora are unknown. The intestine is colonized by a large number of microorganisms, defined as gut microbiome or microbiota.⁴⁴ Because the liver and intestine are closely connected through the portal vein, intestinal microbes and toxins can easily enter the liver, with pathogenic implications.⁴⁵ Intestinal microflora is closely related to liver disease, and relevant research reports are extensive,⁴⁶ but it remains to be elucidated whether any disturbance in the gut microbiota is directly linked to hepatotoxicity induced by A. adenophora.

Despite its toxicological action, A. adenophora contains many bioactivity with potential therapeutic applications. For instance, the extract from A. adenophora has antitumor effects,⁴⁷ and inhibits cancer cell proliferation by triggering the apoptotic pathway.⁴⁸ Furthermore, A. adenophora can also be used as a livestock feed after detoxification. However, a number of bioactive compounds still remain to be identified and characterized, before incorporating A. adenophora in clinical use. The invasiveness of A. adenophora in arable lands is difficult to control, and will require extensive efforts.⁴⁰

At present, there are many basic studies on the comprehensive development and utilization of A. adenophora. However, the extracts of functional active components from A. adenophora are mostly crude extracts. The identification technology, active mechanism and in-depth utilization of monomer chemical components need to be further strengthened. In addition, A. adenophora has a wide range of biological activities, and the specific mechanism of its function needs further detailed study.

Conclusions

The toxicity and potential utilization of A. adenophora were reviewed in this paper. Our concluding recommendation would be to utilize the double attributes of toxicity and drug properties of A. adenophora, and turn waste into treasure through reasonable development in the future!

Supplemental Material

sj-pdf-1-smo-10.1177_20503121231167964 – Supplemental material for Biological properties of active compounds from Ageratina adenophora

Supplemental material, sj-pdf-1-smo-10.1177_20503121231167964 for Biological properties of active compounds from Ageratina adenophora by Wei SUN, Shan-shan LIU and Chen-chen ZHAO in SAGE Open Medicine

Footnotes

Acknowledgements

Many thanks to Samuel Kumi Okyere from Wayne University for his advice and guidance in writing this paper.

Author contributions

The manuscript was written by the contribution of Wei Sun and Shanshan Liu, Chenchen ZHAO corrected the writing content and revised the grammar of the manuscript. All of the authors have approved the final version of the manuscript.

Declaration of conflicting interests

The author(s) declared no potential conflicts of interest with respect to the research, authorship, and/or publication of this article.

Funding

The author(s) received no financial support for the research, authorship, and/or publication of this article. This work was supported by Guizhou Science and Technology Planning Project (grant no. [2019]1456) and Cultivation of high-level innovative talents in Guizhou Province (grant no. 2022-(2020)-045).

Ethics approval

Not applicable

Informed consent

Not applicable

ORCID iD

Wei SUN

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