Sage Journals: Discover world-class research

Abstract

Sinonasal squamous cell carcinoma (SNSCC) is one of the least frequent carcinomas in the head and neck and accounts for 60% to 75% of sinonasal malignancies. The role of long non-coding RNAs (lncRNAs) in cancer development has drawn great attention over the years. The current study intended to assess the role and specific mechanism of lncRNA double homeobox A pseudogene 8 (DUXAP8) in SNSCC. Quantitative real-time PCR (qRT-PCR) analysis was implemented to assess the expression level of DUXAP8, microRNA-584-5p (miR-584-5p), and fibronectin type III domain containing 3B (FNDC3B). Proliferation assays included colony formation assay, Cell Counting Kit-8 (CCK-8) assay, and 5-ethynyl-2′-deoxyuridine (EdU) assay. Transwell assays were implemented to monitor cell migration and invasion. Cell apoptosis was evaluated via terminal deoxynucleotidyl transferase (TdT) dUTP nick-end labeling (TUNEL) and JC-1 experiments. Mechanism experiments included RNA pull-down assay, RNA binding protein immunoprecipitation (RIP) assay, and luciferase reporter assay. DUXAP8 is overexpressed in SNSCC cells. Functionally, DUXAP8 silencing suppresses the malignant progression of SNSCC. Furthermore, DUXAP8 up-regulates the expression of FNDC3B via sponging miR-584-5p. Rescue experiments demonstrated that DUXAP8 mediates the progression of SNSCC via up-regulating FNDC3B expression. In conclusion, DUXAP8 acts as an oncogene in SNSCC, which may be a new molecular marker for SNSCC.

Keywords

DUXAP8 miR-584-5p FNDC3B sinonasal squamous cell carcinoma

Get full access to this article

View all access options for this article.

References

Ansa

Goodman

Ward

, et al. Paranasal sinus squamous cell carcinoma incidence and survival based on surveillance, epidemiology, and end results data, 1973 to 2009. Cancer. 2013;119:2602–2610. DOI: 10.1002/cncr.28108.

Turner

Reh

. Incidence and survival in patients with sinonasal cancer: a historical analysis of population-based data. Head Neck. 2012;34:877–885. DOI: 10.1002/hed.21830.

Lewis

Jr . Sinonasal squamous cell carcinoma: a review with emphasis on emerging histologic subtypes and the role of human papillomavirus. Head Neck Pathol. 2016;10:60–67. DOI: 10.1007/s12105-016-0692-y.

Sanghvi

Khan

Patel

, et al. Epidemiology of sinonasal squamous cell carcinoma: a comprehensive analysis of 4994 patients. Laryngoscope. 2014;124:76–83. DOI: 10.1002/lary.24264.

Michel

Fakhry

Mancini

, et al. Sinonasal squamous cell carcinomas: clinical outcomes and predictive factors. Int J Oral Maxillofac Surg. 2014;43:1–6. DOI: 10.1016/j.ijom.2013.07.741.

Djebali

Davis

Merkel

, et al. Landscape of transcription in human cells. Nature. 2012;489:101–108. DOI: 10.1038/nature11233.

Iyer

Niknafs

Malik

, et al. The landscape of long noncoding RNAs in the human transcriptome. Nat Genet. 2015;47:199–208. DOI: 10.1038/ng.3192.

Liu

Wang

, et al. Long non-coding RNA CHRF promotes proliferation and mesenchymal transition (EMT) in prostate cancer cell line PC3 requiring up-regulating microRNA-10b. Biol Chem. 2018. DOI: 10.1515/hsz-2018-0380.

Meng

Cai

, et al. Long noncoding RNA ZEB1-AS1 promotes the tumorigenesis of glioma cancer cells by modulating the miR-200c/141-ZEB1 axis. Am J Transl Res. 2018;10:3395–3412.

10.

Chen

, et al. Long non-coding RNA XIST promotes the development of esophageal cancer by sponging miR-494 to regulate CDK6 expression. Biomed Pharmacother. 2019;109:2228–2236. DOI: 10.1016/j.biopha.2018.11.049.

11.

Meng

Pan

, et al. Long noncoding RNA DUXAP8 regulates proliferation and apoptosis of ovarian cancer cells via targeting miR-590-5p. Hum Cell. 2020;33:1240–1251. DOI: 10.1007/s13577-020-00398-8.

12.

Huang

, et al. The pseudogene DUXAP8 promotes colorectal cancer cell proliferation, invasion, and migration by inducing epithelial-mesenchymal transition through interacting with EZH2 and H3K27me3. Onco Targets Ther. 2020;13:11059–11070. DOI: 10.2147/ott.S235643.

13.

Yin

Hua

Wang

, et al. Long non-coding RNA DUXAP8 facilitates cell viability, migration, and glycolysis in non-small-cell lung cancer via regulating HK2 and LDHA by inhibition of miR-409-3p. Onco Targets Ther. 2020;13:7111–7123. DOI: 10.2147/ott.S243542.

14.

Tay

Rinn

Pandolfi

. The multilayered complexity of ceRNA crosstalk and competition. Nature. 2014;505:344–352. DOI: 10.1038/nature12986.

15.

Zhang

, et al. LncRNA MT1JP functions as a ceRNA in regulating FBXW7 through competitively binding to miR-92a-3p in gastric cancer. Mol Cancer. 2018;17:87. DOI: 10.1186/s12943-018-0829-6.

16.

Liang

Han

, et al. LncRNA PTAR promotes EMT and invasion-metastasis in serous ovarian cancer by competitively binding miR-101-3p to regulate ZEB1 expression. Mol Cancer. 2018;17:119. DOI: 10.1186/s12943-018-0870-5.

17.

Zheng

Zhou

, et al. Long noncoding RNA FAM225A promotes nasopharyngeal carcinoma tumorigenesis and metastasis by acting as ceRNA to sponge miR-590-3p/miR-1275 and upregulate ITGB3. Cancer Res. 2019;79:4612–4626. DOI: 10.1158/0008-5472.Can-19-0799.

18.

Lin

Chen

, et al. FNDC3B promotes cell migration and tumor metastasis in hepatocellular carcinoma. Oncotarget. 2016;7:49498–49508. DOI: 10.18632/oncotarget.10374.

19.

, et al. A novel LncRNA, AC091729.7 promotes sinonasal squamous cell carcinomas proliferation and invasion through binding SRSF2. Front Oncol. 2019;9:1575. DOI: 10.3389/fonc.2019.01575.

20.

Kang

. Down-regulating NEAT1 inhibited the viability and vasculogenic mimicry formation of sinonasal squamous cell carcinoma cells via miR-195-5p/VEGFA axis. Biosci Rep. 2020;40. DOI: 10.1042/bsr20201373.

21.

Liang

Wang

Zhang

, et al. Long non-coding RNA Duxap8 facilitates cell proliferation and induces apoptosis in colorectal cancer via miR-519b/ZNF277 axis. Onco Targets Ther. 2021;14:4693–4703. DOI: 10.2147/ott.S301233.

22.

Pang

Yang

. lncRNA DUXAP8 inhibits papillary thyroid carcinoma cell apoptosis via sponging the miR-20b-5p/SOS1 axis. Oncol Rep. 2021;45. DOI: 10.3892/or.2021.8015.

23.

Chen

Gao

Liang

. DUXAP8 knockdown inhibits the development of melanoma by regulating the miR-3182/NUPR1 pathway. Oncol Lett. 2021;22:495. DOI: 10.3892/ol.2021.12756.

24.

Guo

Zheng

Wang

, et al. Mir-584-5p regulates migration and invasion in non-small cell lung cancer cell lines through regulation of MMP-14. Mol Med Rep. 2019;19:1747–1752. DOI: 10.3892/mmr.2019.9813.

25.

Wei

Wang

, et al. miR-584-5p regulates hepatocellular carcinoma cell migration and invasion through targeting KCNE2. Mol Genet Genomic Med. 2019;7:e702. DOI: 10.1002/mgg3.702.

26.

Zhong

Zhang

Hong

, et al. FNDC3B promotes epithelial-mesenchymal transition in tongue squamous cell carcinoma cells in a hypoxic microenvironment. Oncol Rep. 2018;39:1853–1859. DOI: 10.3892/or.2018.6231.

27.

Qiu

. Potential mechanisms of microRNA-129-5p in inhibiting cell processes including viability, proliferation, migration and invasiveness of glioblastoma cells U87 through targeting FNDC3B. Biomed Pharmacother. 2017;87:405–411. DOI: 10.1016/j.biopha.2016.12.100.

Supplementary Material

Please find the following supplemental material available below.

For Open Access articles published under a Creative Commons License, all supplemental material carries the same license as the article it is associated with.

For non-Open Access articles published, all supplemental material carries a non-exclusive license, and permission requests for re-use of supplemental material or any part of supplemental material shall be sent directly to the copyright owner as specified in the copyright notice associated with the article.

0.00 MB

2.01 MB

Long Non-Coding RNA DUXAP8 Acts as an Oncogene in Sinonasal Squamous Cell Carcinoma Through miR-584-5p/FNDC3B Pathway

Abstract

Keywords

Get full access to this article

References

Supplementary Material