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Abstract

Background

Acacia honey is known to have antioxidant, immune-modulatory, and antiproliferative properties. Nasal fibroblasts participate in local immune responses that control the recruitment of inflammatory cells and the production of extracellular matrix.

Objectives

The aim of this study was to determine the effect of acacia honey on myofibroblast differentiation and matrix metalloproteinase-9 (MMP-9) production in nasal polyp fibroblasts.

Methods

Primary nasal fibroblasts were isolated from nasal polyps and treated with transforming growth factor-beta 1 (TGF-β1). Reverse transcription-polymerase chain reaction and Western blot analysis were then performed to determine α-smooth muscle actin (α-SMA), tissue inhibitors of matrix metalloproteinase-1, and MMP-9 mRNA expression and protein production in nasal polyp fibroblasts. Phosphorylated Smad (pSmad) 2/3 and phosphorylated adenosine monophosphate-activated protein kinase (pAMPK) were then determined by Western blotting.

Results

TGF-β1 stimulation increased α-SMA and MMP-9 mRNA expression and protein production in nasal polyp fibroblasts. Acacia honey effectively suppressed α-SMA and MMP-9 mRNA expression and protein production. It also prevented phosphorylation of Smad 2/3 and AMPK.

Conclusion

Acacia honey can inhibit TGF-β1-induced myofibroblast differentiation and MMP-9 production in nasal fibroblasts. These results suggest that acacia honey might be useful for inhibiting nasal polyp formation.

Keywords

acacia honey nasal fibroblast matrix metalloproteinase-9 myofibroblast extracellular matrix

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References

Fokkens

Lund

Mullol

, et al. EPOS 2012: European position paper on rhinosinusitis and nasal polyps 2012. A summary for otorhinolaryngologists. Rhinology. 2012; 50:1–12.

Lechapt-Zalcman

Coste

d’Ortho

, et al. Increased expression of matrix metalloproteinase-9 in nasal polyps. J Pathol. 2001; 193:233–241.

Watelet

Bachert

Claeys

Van Cauwenberge

Matrix metalloproteinases MMP-7, MMP-9 and their tissue inhibitor TIMP-1: expression in chronic sinusitis vs nasal polyposis.

Allergy. 2004; 59:54–60.

Wang

Escudier

Roudot-Thoraval

Abd-Al Samad

Peynegre

Coste

Myofibroblast accumulation induced by transforming growth factor-beta is involved in the pathogenesis of nasal polyps.

Laryngoscope. 1997; 107:926–931.

Al-Waili

Salom

Al-Ghamdi

AA.

Honey for wound healing, ulcers, and burns; data supporting its use in clinical practice.

ScientificWorldJournal. 2011; 11:766–787.

Erejuwa

Sulaiman

Wahab

MS.

Effects of honey and its mechanisms of action on the development and progression of cancer.

Molecules. 2014; 19:2497–2522.

Leong

Herst

Harper

JL.

Indigenous New Zealand honeys exhibit multiple anti-inflammatory activities.

Innate Immun. 2012; 18:459–466.

Gannabathula

Skinner

Rosendale

, et al. Arabinogalactan proteins contribute to the immunostimulatory properties of New Zealand honeys. Immunopharmacol Immunotoxicol. 2012; 34:598–607.

Kropf

Golob

Necemer

, et al. Carbon and nitrogen natural stable isotopes in Slovene honey: adulteration and botanical and geographical aspects. J Agric Food Chem. 2010; 58:12794–12803.

10.

Iftikhar

Arshad

Rasheed

Amraiz

Anwar

Gulfraz

Effects of acacia honey on wound healing in various rat models.

Phytother Res. 2010; 24:583–586.

11.

Abd Ghafar

Ker-Woon

Hui

Mohd Yusof

Wan Ngah

. Acacia honey accelerates in vitro corneal ulcer wound healing model.

BMC Complement Altern Med. 2016; 16:259.

12.

Orlandi

Kingdom

Hwang

, et al. International consensus statement on allergy and rhinology: Rhinosinusitis. Int Forum Allergy Rhinol. 2016; 6:S22–S209.

13.

Tokunaga

Sakashita

Haruna

, et al. Novel scoring system and algorithm for classifying chronic rhinosinusitis: the JESREC Study. Allergy. 2015; 70:995–1003.

14.

Jordana

Schulman

McSharry

, et al. Heterogeneous proliferative characteristics of human adult lung fibroblast lines and clonally derived fibroblasts from control and fibrotic tissue. Am Rev Respir Dis. 1988; 137:579–584.

15.

Kuhar

Tajudeen

Mahdavinia

Gattuso

Ghai

Batra

PS.

Inflammatory infiltrate and mucosal remodeling in chronic rhinosinusitis with and without polyps: structured histopathologic analysis.

Int Forum Allergy Rhinol. 2017; 7:679–689.

16.

Cho

Moon

Park

, et al. Epigenetic regulation of myofibroblast differentiation and extracellular matrix production in nasal polyp-derived fibroblasts. Clin Exp Allergy. 2012; 42:872–882.

17.

Nonaka

Pawankar

Fukumoto

Yagi

Heterogeneous response of nasal and lung fibroblasts to transforming growth factor-beta 1.

Clin Exp Allergy. 2008; 38:812–821.

18.

Hussein

Yusoff

Makpol

Yusof

YA.

Antioxidant capacities and total phenolic contents increase with gamma irradiation in two types of Malaysian honey.

Molecules. 2011; 16:6378–6395.

19.

Muhammad

Odunola

Ibrahim

, et al. Potential biological activity of acacia honey. Front Biosci (Elite Ed). 2016; 8:351–357.

20.

Park

Hong

, et al. Metformin reduces TGF-beta1-induced extracellular matrix production in nasal polyp-derived fibroblasts. Otolaryngol Head Neck Surg. 2014; 150:148–153.

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