Chemical Profiling,Antioxidant and Antibacterial Activities of Essential Oil From Englerastrum gracillimum Th. C. E. Fries Growing in Niger

Abstract

In the present study, the chemical composition, antioxidant and antibacterial activities of the essential oil obtained by hydrodistillation of the aerial parts of Englerastrum gracillimum Th. C. E. Fries growing in Niger were investigated. Gas chromatography-mass spectrometry (GC–MS) analysis of the essential oil resulted in the identification of 42 compounds representing 97.9% of the total oil constituents. The major compounds of the essential oil were: α-humulene (30.5%), followed by cubenol (19.8%), γ-muurolene (14.0%), (E)-β-caryophyllene (5.8%), β-gurjunene (5.2%), and curzerene (4.9%). The antioxidant activity of the essential oil was determined by using the free radical-scavenging activity (2,2 diphenyl-1-picrylhydrazyl: DPPH˙) and ferric reducing antioxidant power assays. The essential oil showed good antioxidant potential with both methods. The antibacterial activity was evaluated against multi-resistant Acinetobacter baumannii P1483, extended-spectrum β-lactamase (ESBL)-Escherichia coli Bu8566, Salmonella spp. H1548, Proteus mirabilis Bu190, Enterobacter cloacae Bu147, Pseudomonas aeruginosa (ATCC 27853), Escherichia coli (ATCC 25922), Klebsiella pneumoniae (ATCC 700603), methicillin-resistant Staphylococcus aureus P1123, Enterococcus faecium H3434, and Staphylococcus aureus (ATCC 25923) using the agar disc diffusion and microdilution methods. The essential oil exhibited good antibacterial activity. The highest antibacterial effect was observed against Staphylococcus aureus ATCC 25923 and methicillin-resistant Staphylococcus aureus P1123 with minimal inhibitory concentration (MIC) values of 0.03 mg/mL and 0.06 mg/mL, respectively. These data are of scientific importance for the valorization of aromatic and medicinal plants of Niger, especially E. gracillimum. This study reports for the first time the antioxidant and antibacterial activities of this essential oil.

Keywords

essential oil chemical composition antioxidant activity antibacterial activity

Recently, essential oils (EO) have been widely investigated by researchers as valuable sources of bioactive compounds, and for their diverse applications in industrial fields. Essential oils are complex mixtures of volatile compounds obtained most commonly by steam or hydro-distillation. There has been great research interest in the assessment of the chemical composition and biological properties of essential oils from plants. Englerastrum gracillimum Th. C. E. Fries, known as Coleus gracillimus (T.C.E. Fr.),¹ and Plectranthus gracillimus (T.C.E.Fr.)² is an annual herb of the Lamiaceae family present in Niger. This plant grows in the Sudanese zone of Africa and is traditionally used as an insect repellent and love filter by nomads.³ However, to the best of our knowledge, there are no data published in the literature on the biological activities of E. gracillimum essential oil. Therefore, the purpose of this study was to characterize the chemical composition of the essential oil isolated from the aerial parts of this plant by hydrodistillation, and to evaluate its antioxidant and antibacterial activities. Then, the results could be used for further applications of this plant as a source of valuable products.

Results and Discussion

Chemical Composition of the EO

The essential oil yield obtained from the aerial parts of E. gracillimum by hydrodistillation was 0.4%; the chemical composition of the oil, determined by GC-MS, is presented in Table 1. A total of 42 components were identified, representing 97.95% of the total oil constituents. The major compounds were: α-humulene (30.5%), cubenol (19.8%), γ-muurolene (14.0%), (E)-β-caryophyllene (5.8%), β-gurjunene (5.2%), and curzerene (4.9%). The EO’s main classes of compounds were sesquiterpene hydrocarbons (61.2%) and oxygenated sesquiterpenes (32.7%). In contrast, monoterpene hydrocarbons and oxygenated monoterpenes were found in small quantities, with amounts of 3.2% and 0.2%, respectively. Our results are in agreement with those of Djibo et al.,³ who also found sesquiterpene hydrocarbons (32.4%) and oxygenated sesquiterpenes (29.4%) as the main classes of compounds in the essential oil obtained from aerial parts of E. gracillimum from Niger. They identified 28 compounds, the major ones being humulene 1,2-epoxide (8.6%), δ-cadinene (7.5%), α-cadinol (7.0%), α-humulene (6.6%), caryophyllene oxide (6.4%), and β-caryophyllene (4.9%). There are minor differences in the chemical composition in terms of the major constituents of the two essential oils of Englerastrum gracillimum from Niger. Also, we found more components in our study. These differences could be due to the different habitat, sample collection times, genetic factors, soil composition, environmental conditions, age of the plant, and other factors that can influence the quality and quantity of oil constituents. It has been reported that variation in the EO chemical composition could be due to the developmental stage of growth,⁴ culture and harvest time,⁵ soil composition, environment,^6,7 genetic factors,⁸ and geographical location.⁹

Table 1

Chemical Composition of Englerastrum Gracillimum Essential Oil.

No.	RI^a	RI^b	^cCompound	Percentage (%)
1	948	939	α-Pinene	0.5
2	956	954	Camphene	0.1
3	973	979	β-Pinene	0.9
4	1024	1025	p-Cymene	0.1
5	1038	1029	Limonene	0.8
6	1040	1037	(Z)-β-Ocimene	0.6
7	1056	1050	(E)-β-Ocimene	0.1
8	1058	1059	γ-Terpinene	0.08
9	1168	1177	Terpinen-4-ol	0.2
10	1343	1351	α-Cubebene	0.1
11	1374	1376	α-Copaene	0.08
12	1382	1381	Geranyl acetate	0.08
13	1398	1390	β-Elemene	0.9
14	1397	1407	Longifolene	0.1
15	1419	1409	α-Gurjunene	0.1
16	1417	1419	( E)-β-Caryophyllene	5.8
17	1435	1433	β-Gurjunene	5.2
18	1430	1434	trans-α-Bergamotene	0.4
19	1445	1436	γ-Elemene	1.2
20	1459	1454	α-Humulene	30.5
21	1469	1479	γ-Muurolene	14.0
22	1480	1484	α-Amorphene	0.3
23	1486	1490	β-Selinene	0.1
24	1494	1498	α-Selinene	0.6
25	1492	1499	Curzerene	4.9
26	1507	1509	Germacrene A	0.1
27	1526	1522	β-Sesquiphellandrene	0.5
28	1565	1561	Germacrene B	1.0
29	1564	1563	(E)-Nerolidol	0.8
30	1570	1568	Palustrol	0.4
31	1576	1578	Spathulenol	0.6
32	1580	1583	Caryophyllene oxide	1.5
33	1637	1646	Cubenol	19.8
34	1644	1650	β-Eudesmol	1.9
35	1660	1654	α-Cadinol	1.2
36	1675	1685	α-Bisabolol	0.8
37	1684	1693	Germacrone	0.7
38	1697	1700	Eudesm-7(11)-en-4-ol	0.2
39	1854	1845	Phytone	0.10
40	1955	1959	Geranyl benzoate	0.09
41	2109	2100	n-Heneicosane	0.2
42	2407	2400	n-Tetracosane	0.1
Sub-totals (%)
Monoterpene hydrocarbons 3.2
Oxygenated monoterpenes 0.2
Sesquiterpene hydrocarbons 61.2
Oxygenated sesquiterpenes 32.7
Other components 0.6
Total identified (%) 97.9

RI^a: experimental retention indices.

RI^b: retention indices from literature.

^cCompounds are listed in order of elution from the column.

Antioxidant Activities

Antioxidant activities of the essential oil were evaluated by using the DPPH^• method and ferric reducing antioxidant power assay at concentrations ranging from 0.1 to 1 mg/mL.

DPPH^• is a stable free radical, which by accepting a hydrogen radical becomes a stable diamagnetic molecule.¹⁰ The DPPH^• reduction capacity was evaluated by the decrease in absorbance at 517 nm induced by the essential oil of E. gracillimum. The IC₅₀ value obtained for the essential oil was 3.12 mg/mL, while that of ascorbic acid, the positive control, was 0.044 mg/mL. The antioxidant activity of the essential oil was also evaluated by the ferric reducing antioxidant power assay. The result for this method is expressed in terms of an EC₅₀ value, which is the concentration of the essential oil giving an absorbance of 0.5 for reducing power. The EC₅₀ value obtained for the oil was 1.02 mg/mL and for ascorbic acid, the positive control, 0.028 mg/mL. The essential oil exhibited good antioxidant activities in both assays. In the DPPH^• test, the IC₅₀ value of 3.12 mg/mL suggested a moderate free radical-scavenging capacity. In the ferric reducing antioxidant power assay, the EC₅₀ value indicated a good reducing power capacity of the essential oil. This antioxidant potential of E. gracillimum essential oil can be attributed to its chemical constituents, such as γ-terpinene, α‐copaene, limonene, caryophyllene oxide, α‐pinene, p-cymene, and β-selinene, which have been reported to have antioxidant properties.^11

-14 The essential oil antioxidant activities represent the product of synergistic, additive, and/or antagonistic effects, because they are complex combinations of several classes of compounds.¹⁵ To our best knowledge, this is the first report of antioxidant activities of the essential oil of E. gracillimum.

Antibacterial Activity

The EO of Englerastrum gracillimum aerial parts was examined for its antibacterial activity against eleven pathogenic bacterial strains: Gram-negative: multi-resistant Acinetobacter baumannii P1483, extended-spectrum β-lactamase (ESBL)-Escherichia coli Bu8566, Salmonella spp. H1548, Proteus mirabilis Bu190, Enterobacter cloacae Bu147, Pseudomonas aeruginosa (ATCC 27853), Escherichia coli (ATCC 25922), Klebsiella pneumoniae (ATCC 700603) and Gram-positive: methicillin-resistant Staphylococcus aureus P1123, Enterococcus faecium H3434 and Staphylococcus aureus (ATCC 25923).

Agar Disc Diffusion Assay

The antibacterial activity of the EO was screened against various human pathogenic Gram-positive and Gram-negative bacteria by measuring the inhibition zone diameter of bacterial growth around the discs by the agar disc diffusion method. The inhibitory capacity of the EO and chloramphenicol as a positive control was estimated as described by Ponce et al.¹⁶ The results from the agar disc diffusion assay are presented in Figure 1. The EO demonstrated a variable level of antibacterial capacity against the micro-organisms tested. Staphylococcus aureus ATCC 25923 was the most sensitive strain to the EO of E. gracillimum, with the strongest inhibition zone of 44 mm, followed by methicillin-resistant Staphylococcus aureus P1123, and Enterococcus faecium H3434, with inhibition zones of 35 and 25 mm, respectively. The EO was strongly active against all the Gram-positive bacteria tested. However, only a weak inhibitory effect was observed against multi-resistant Acinetobacter baumannii P1483, with an inhibition zone of 9 mm. Also, seven Gram-negative bacterial strains: Salmonella spp. H1548, extended-spectrum β-lactamase (ESBL)-Escherichia coli Bu8566, Enterobacter cloacae Bu147, Proteus mirabilis Bu190, Pseudomonas aeruginosa ATCC 27853, Escherichia coli ATCC 25922, and Klebsiella pneumoniae ATCC 700603, were resistant to the EO of E. gracillimum. Chloramphenicol was more active than the EO of E. gracillimum against Gram-negative bacteria. A higher inhibition zone for chloramphenicol was obtained for Salmonella spp. H1548 (30 mm). Enterococcus faecium H3434, Pseudomonas aeruginosa ATCC 27853, and Enterobacter cloacae Bu147 were not sensitive to chloramphenicol. It is important to notice that the EO proved to be more active than chloramphenicol against Gram-positive bacteria. These findings indicate the susceptibility of the Gram-positive bacteria to the EO investigated, and are in accordance with many other studies, which have reported that Gram-positive bacteria are more susceptible to the inhibitory effects of essential oils than Gram-negative ones.^17
-19 According to the literature, the resistance of Gram-negative bacteria to essential oils has been attributed to the presence of a hydrophilic external lipopolysaccharide membrane that blocks the penetration of hydrophobic molecules of oil into the cells.^20
-22

Figure 1

Diameter of inhibition zone (mm) from the action of chloramphenicol and Englerastrum gracillimum essential oil against the pathogenic bacteria.

Microdilution Assay

The determination of the minimal inhibitory concentration (MIC) and minimal bactericidal concentration (MBC) was assessed by the microdilution assay. The MIC was determined for the bacterial strains that were sensitive to the essential oil in the agar disc diffusion assay. The MBC was determined from the inoculate aliquots of culture for the wells that showed no visible growth of bacteria from the MIC assessment. The MIC and MBC assessments are summarized in Table 2. The antibacterial activity of E. gracillimum EO was variable according to the micro-organism tested. The highest antibacterial activity was obtained for Staphylococcus aureus ATCC 25923, reflected by the lowest MIC value of 0.03 mg/mL. Also, the EO showed strong antibacterial activity against methicillin-resistant Staphylococcus aureus P1123, evidenced by a MIC value of 00.6 mg/mL. The lowest MIC value for Chloramphenicol was obtained for Salmonella spp. H1548 (0.03 mg/mL), indicating the strongest antibacterial activity of Chloramphenicol against this strain. The MBC values were identical to or greater than the MIC values. The significant antibacterial effect of E. gracillimum EO might be attributed to constituents such as α-humulene, terpinen-4-ol, α-pinene, γ-terpinene, (E)-β-caryophyllene, p-cymene, α-cadinol, β-pinene, and limonene, which have been reported to possess antibacterial properties,.^9,23

-27 However, essential oils are a mixture of various active compounds, therefore, the synergistic and additive effects of these compounds should be taken into account for the evaluation of their antibacterial activities.^28

-31 We have not found any data reported on the antibacterial activities of E. gracillimum essential oil.

Table 2

MIC and MBC Values for Englerastrum gracillimum Essential Oil and Chloramphenicol Against the Pathogenic Bacteria Tested.

Bacteria	ChloramphenicolMIC (mg/mL)	Essential oil
Bacteria	ChloramphenicolMIC (mg/mL)	MIC (mg/mL)	MBC (mg/mL)
Methicillin-resistant Staphylococcus aureus P1123	0.9	0.06	0.06
Enterococcus faecium H3434	-	0.08	0.5
Staphylococcus aureus ATCC 25923	0.08	0.03	0.03
Multi-resistant Acinetobacter baumannii P1483	1.12	3	4
Proteus mirabilis Bu190	0.05	-	-
Salmonella spp. H1548	0.03	-	-
E. coli ATCC 25922	0.09	-	-
ESBL-E. coli Bu8566	0.06	-	-
Klebsiella pneumoniae ATCC 700603	0.12	-	-
Pseudomonas aeruginosa ATCC 27853	-	-	-
Enterobacter cloacae Bu147	-	-	-

- “not active”. Abbreviations: ATCC, American Type Culture Collection; E. coli, Escherichia coli;EO, Essential oil; ESBL, Extended-spectrum β-lactamase; MBC, minimum bactericidal concentration; MIC, minimum inhibitory concentration.

Conclusions

In this study, the chemical composition analysis of Englerastrum gracillimum essential oil led to the identify of 42 compounds, with a predominance of sesquiterpene hydrocarbons (61.2%) and oxygenated sesquiterpenes (32.7%). The essential oil showed good antioxidant potential. These results suggest that the EO of E. gracillimum could be considered as a significant source of natural antioxidant agents. Also, the EO demonstrated a good antibacterial effect against the human pathogenic bacteria tested, especially against the Gram-positive ones. These results showed that the EO of E. gracillimum is a good source of bioactive compounds with potential antioxidant and antibacterial activities. Therefore, E. gracillimum could be a possible alternative for the treatment of diseases related to oxidative stress and to treat some infectious diseases caused by human pathogenic bacteria. This report is the first study on the chemical composition and biological activities of E. gracillimum essential oil from Niger, and provides a scientific basis for the use of this plant in traditional medicine.

Materials and Methods

Antibiotics, Broth, and Chemical Reagents

Dimethyl sulfoxide (DMSO), 2,2 diphenyl-1-picrylhydrazyl (DPPH^•), ethanol, ascorbic acid, iron III chloride (FeCl₃), potassium ferricyanide (K₃Fe(CN)₆), and all others chemical reagents used in this study were obtained from Sigma-Aldrich. Standard antibiotics were purchased from OXOID (UK), while the sterile 96-well microtiter plates were purchased from CITOTEST (CHINA). Sterile paper discs and culture media (Nutrient broth, Nutrient agar) were products of Becton, Dickinson & Co (New Jersey, USA).

Plant Material and Essential Oil Extraction

The aerial parts of Englerastrum gracillimum were collected from Lido, located in the South of Niger (altitude 232 m, latitude (N) 12°53’058’’, longitude (E) 003°44’446’’) during October 2018. The plant materials were air-dried at room temperature away from light. The plant was identified by a botanist at Abdou Moumouni University, Niamey (Niger), and a voucher number (NA/06) was given to the plant. The essential oil (EO) was obtained by hydrodistillation using a Clevenger type apparatus.³² One hoursundred g of dried aerial parts were hydrodistilled for 3 hours. The obtained essential oil was stored at 4 °C until analysis.

GC-MS Analysis of Essential Oil

The essential oil (EO) chemical composition was analyzed by a GC-MS technique, which used a GCMS-QP2010SE Gas Chromatograph/Mass Spectrometer (SHIMADZU CORPORATION, Columbia-USA) equipped with two columns, a Zebron ZB-5ms (20 m × 0.18 mm x 0.18 µm) and Zebron ZB-WAX (20 m × 0.18 mm × 0.18 µm), and coupled with a Mass Analyzer single quadrupole system, operating in Scan/SIM mode. The initial temperature of the columns was maintained at 50 °C for 3 minutes and increased at 2 °C/min until 280 °C, and then maintained at 280 °C for 30 minutes. The sample was injected by using split mode (1/30), and the volume injected was 1 µL. The injector temperature was 280 °C and helium was used as the carrier gas (1 mL/min). The system operated in the electron impact (EI) mode, and data were collected over a mass range of 20 to 500; scan time 0.2 s. GC/FID analysis was performed under the same experimental conditions as described for the GC-MS. Constituents were identified by comparing their retention indices (RI) determined by using a homologous series of n-alkanes and mass spectral fragmentation patterns with those stored in the NIST08.LIB mass spectral libraries of the GC–MS data system and from literature data.³³ The concentrations of the chemical components were calculated based on GC peak areas without using correction factors.

Antioxidant Activity

DPPH^• Radical-Scavenging Activity

The 2,2 diphenyl-1-picrylhydrazyl radical-scavenging activity of the essential oil was evaluated according to the method reported by Blois, with slight modifications.³⁴ The samples were diluted to prepare a range of concentrations from 0.1 to 1 mg/mL. One mL of each concentration of the different samples was added to 2 ml of DPPH^• (0.2 mmol) ethanolic solution. The mixtures were left in the dark at room temperature. After 30 minutes of incubation, the absorbance of the mixture was measured at 517 nm using a spectrophotometer {Uviline 9400 (SECOMAM)} against a blank solution (1 ml of ethanol +2 ml of DPPH^• solution). Ascorbic acid was used as a positive control. The antioxidant activity of all the test samples was expressed as IC₅₀ (mg/mL), defined as the concentration of the antioxidant needed to scavenge 50% of DPPH^• present in the test solution. The percentage of inhibition of DPPH^• free radical-scavenging activity was calculated by the followed equation:

D P P H^{∙} s c a v e n g i n g e f f e c t (%) = [(A_{0} - A_{1}) ∕ A_{0}] \times 100

Where A₀ is the absorbance of the blank solution and A₁ is the absorbance of the test sample.

Ferric Reducing Antioxidant Power Assay

The capacity of the essential oil to reduce Fe³⁺ was assayed with the method reported by Yıldırım et al.³⁵ Different concentrations of the essential oil were prepared in absolute ethanol: 0.1, 0.2, 0.4, 0.6, 0.8, and 1 mg/mL. One mL of the different sample concentrations was added to 2.5 ml of phosphate buffer (0.2 M, pH = 6.6) and 2.5 ml of K₃Fe(CN)₆ solution (1%). The mixture was incubated at 50 °C in a water bath (DIGITERM 100, J.P. SELECTA, S.A; Spain) for 30 minutes. After incubation, the mixture was left at room temperature, 2.5 ml of trichloroacetic acid (10%) was added, and then centrifuged (HERMLE Z323K, Germany) at 3000 rpm for 10 minutes. Finally, 2.5 ml of the upper layer solution was mixed with 2.5 ml of distilled water and 0.5 ml of FeCl₃ (0.1%); the absorbance was measured at 700 nm (A₇₀₀) with a Uviline 9400 spectrophotometer (SECOMAM). The antioxidant activity of all the test samples was expressed as EC₅₀ (mg/mL). The EC₅₀ value corresponds to the effective concentration of the essential oil giving an absorbance of 0.5 for reducing power and was determined from linear regression analysis of the graphs of A₇₀₀ against the corresponding essential oil concentrations.³⁶ Ascorbic acid was used as a standard compound.

Antibacterial Activity

The evaluation of the essential oil antibacterial activity was performed at the Laboratory of Microbiology and Virology of Mohamed VI University Hospital Center in Marrakech, Morocco.

Bacterial Strains

The essential oil antibacterial activity was evaluated against eleven pathogenic bacterial strains including Gram-negative: multi-resistant Acinetobacter baumannii P1483, extended-spectrum β-lactamase (ESBL)-Escherichia coli Bu8566, Salmonella spp. H1548, Proteus mirabilis Bu190, Enterobacter cloacae Bu147, Pseudomonas aeruginosa (ATCC 27853), Escherichia coli (ATCC 25922), Klebsiella pneumoniae (ATCC 700603) and Gram-positive: methicillin-resistant Staphylococcus aureus P1123, Enterococcus faecium H3434 and Staphylococcus aureus (ATCC 25923), as reported in our previous published work.³⁷ The reference strains (ATCC: American Type Culture Collection Center, Manassas, Virginia, USA) were obtained from the culture collection of the Laboratory of Microbiology and Virology of Mohamed VI University Hospital Center in Marrakech (Morocco), and all the clinical strains were isolated and identified at the same Laboratory.

Agar Disc Diffusion Assay

The agar disc diffusion assay was used to perform the antibacterial activity of the essential oil according to the method reported by Ali et al.³⁸ To obtain a young culture of bacterial colonies, the test bacteria were seeded on Petri dishes containing Mueller-Hinton agar and incubated for 24 hours at 37 °C. After incubation, these colonies were taken and placed in 3 ml of sterile 0.9% NaCl solution to prepare a bacterial suspension adjusted to 0.5 McFarland (10⁸ CFU/mL) standard turbidity by a spectrophotometer (BD PhoenixSpec). On the surface of the Petri dishes containing the Mueller-Hinton agar seeded with the pathogenic bacteria, sterile Whatman N°1 paper discs (6 mm in diameter) were placed and impregnated with 10 µL of essential oil. Discs impregnated with dimethyl sulfoxide were used as the negative control and chloramphenicol as a positive control. All Petri dishes were incubated for 24 hours at 37 °C for bacterial growth. After incubation, the diameters of the inhibition zones of bacterial growth around the discs were measured in mm; each experiment was made three times.

Microdilution Assay

The microdilution method was used to determine the minimum inhibitory concentration (MIC), as described by Chrysargyris et al., and Selim et al.,^39,40 with slight modifications. A range of concentrations of the essential oil from 0.03 to 10 mg/mL was prepared in dimethyl sulfoxide (DMSO). The inoculum of the bacterial strains was prepared from 24 hours broth cultures and suspensions were adjusted to 0.5 McFarland (10⁸ CFU/mL) standard turbidity. The microdilution assay was carried out using sterile 96-well microtiter plates. The 96 well-plates were prepared by putting 90 µL of Mueller-Hinton broth (MHB) and 10 µL of the inoculum into each well. One hoursundred μL of each concentration of the serial dilution of the essential oil was added and the final volume in each well was 200 µL. The negative control was prepared containing 90 µL of Mueller-Hinton broth (MHB), 10 µL of the inoculum and 100 µL of chloramphenicol. The positive control was also prepared containing 90 µL of Mueller-Hinton broth (MHB), 10 µL of the inoculum, and 100 µL of DMSO in the last wells of each strip. After mixing the wells, they were covered and incubated at 37 °C for 24 hours. The MIC value was defined as the lowest concentration of the essential oil that inhibited the visible growth of the microorganisms tested after 24 hours of incubation. To determine the minimum bactericidal concentration (MBC), the content of each well without any visible growth of bacteria was seeded on Petri dishes containing the Mueller-Hinton agar and incubated for 24 hours at 37 °C.⁴¹ The MBC was considered as the concentration of the essential oil that did not exhibit any viable organism in the culture after 24 hours of incubation.

Footnotes

Acknowledgments

The author(s) are grateful to Professor Jean Luc-Pirat ENSC, Montpellier France; Dr. Nabila Soraa and Ilham Dilagui, Laboratory of Microbiology and Virology, Faculty of Medicine and Pharmacy, Cadi Ayyad University, Marrakech, Morocco.

Declaration of Conflicting Interests

The author(s) declared no potential conflicts of interest with respect to the research, authorship, and/or publication of this article.

Funding

The author(s) received no financial support for the research, authorship, and/or publication of this article.

ORCID iDs

Bala Namata Abba

Amadou Tidjani Ilagouma

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Chemical Profiling,Antioxidant and Antibacterial Activities of Essential Oil From Englerastrum gracillimum Th. C. E. Fries Growing in Niger

Abstract

Keywords

Results and Discussion

Chemical Composition of the EO

Antioxidant Activities

Antibacterial Activity

Agar Disc Diffusion Assay

Microdilution Assay

Conclusions

Materials and Methods

Antibiotics, Broth, and Chemical Reagents

Plant Material and Essential Oil Extraction

GC-MS Analysis of Essential Oil

Antioxidant Activity

DPPH• Radical-Scavenging Activity

Ferric Reducing Antioxidant Power Assay

Antibacterial Activity

Bacterial Strains

Agar Disc Diffusion Assay

Microdilution Assay

Footnotes

Acknowledgments

Declaration of Conflicting Interests

Funding

ORCID iDs

References

DPPH^• Radical-Scavenging Activity