Composition and Chemical Variability of Myrtus communis Leaf Oil From Northwestern Algeria

Abstract

Myrtus communis L. is a shrub growing wild in most countries all around the Mediterranean Sea and myrtle leaf oil is useful in the perfumery and pharmaceutical industries. Nowadays, “chemotyped” essential oils are more and more appreciated. Myrtle oil from eastern and central-north Algeria belong to the “α-pinene-cineole” chemotype. In contrast, Moroccan myrtle oil displayed appreciable content of myrtenyl acetate. Therefore, it appeared crucial to investigate the composition of myrtle leaf oil from northwestern Algeria in order to ensure the homogeneity of the composition of Algerian myrtle leaf oil. The chemical composition of 42 oil samples of M. communis isolated from leaves collected in five locations from Tlemcen Province was investigated by gas chromatography (GC) in combination with retention indices, GC-mass spectroscopy, and ¹³C nuclear magnetic resonance spectroscopy. Yields ranged between 0.13% and 1.04% (w/w). The chemical composition of the oils was largely dominated by monoterpene hydrocarbons, with α-pinene (24.3-59.0%) and 1,8-cineole (13.2-49.5%) being the major compounds followed by limonene (3.2-19.8%). The 42 compositions were submitted to statistical analyses. The combination of hierarchical cluster analysis and principal component analysis allowed the distinction of two groups, the second group being subdivided into two subgroups. Groups and subgroups were differentiated with respect to their contents of α-pinene and 1,8-cineole and, to a lesser extent, of limonene. This study ensures that Algerian myrtle leaf oil belongs to the “α-pinene-cineole” chemotype which displays various biological activities. The observed homogeneity could be a positive point to join the international market of essential oils.

Keywords

Myrtaceae essential oil composition chemical variability α-pinene-cineole chemotype Northwestern Algeria

Despite the importance, the abundance, and the diversity of its flora, Algeria imports most of the essential oils (EOs) and plant extracts used in the country for perfumery, food flavoring, or pharmaceutical purposes. Various projects based on the valorization of Algerian aromatic and medicinal plants at artisanal or industrial scale were born.^1,2 Nowadays, quality of commercialized EOs is a crucial parameter. “Chemotyped” EOs are more and more appreciated, particularly in the domain of aromatherapy.³

Myrtus communis L. grows wild in most countries all around the Mediterranean Sea. EO is isolated from different organs, leaves, flowers, and berries. Leaf oil has an industrial importance, due to its pleasant odor (perfumery) and biological activities (aromatherapy). Biological aspects of Algerian myrtle oil have been recently reported⁴ and reviewed.⁵

Various papers reported on the chemical composition of Algerian myrtle leaf oil. Almost all the papers described the composition of a limited number (1-3) of samples and almost all the investigated oil samples contained α-pinene and 1,8-cineole as main components.⁵ An overview of myrtle oil from eastern and central Algeria has been done.^6,7 Taking into account the relative contents of the two major components α-pinene and 1,8-cineole as well as those, at a lesser extent, of limonene and linalool, statistical analysis allowed the differentiation of two groups and two subgroups.⁷ Similar results were reported for myrtle from Chlef Province. The composition of 11/14 oil samples was dominated by α-pinene and 1,8-cineole while linalool reached high contents for the last three samples.^8,9 Otherwise, a few atypical samples have been reported. For instance, an oil sample from Tizi Ouzou contained 1,8-cineole and cis-geraniol as main components.¹⁰ A sample from Forest of Bissa (Chlef) contained mainly limonene besides linalool, α-pinene, and geranyl acetate.^8,9 In all the reported compositions, myrtenyl acetate was absent or present at trace level. Surprisingly, it has been reported as major component (38.7%) of a myrtle leaf oil sample from Aïn Defla Province, 150 km south-west from Algiers.¹¹ Such a composition that looks really unusual in Algeria is classical for Moroccan myrtle leaf oils.^12-14

As a consequence, it appeared crucial to investigate the composition of myrtle leaf oils from northwestern Algeria, in order to get an overview of the composition of wild Algerian myrtle leaf oil. Plants have been collected in the province of Tlemcen near the Moroccan frontier. Indeed, it could be useful for EO producers and eventual exporters to describe a representative chemotype of Algerian myrtle leaf oil.

Leaves from 42 individual plants have been harvested in the following locations: Aïn Kebira, Beni Khellad, Beni Ouarsous, Honaïne, and Nedroma. Essential oil was isolated using a Clevenger-type apparatus. Yields varied substantially, between 0.13% and 1.04%. Highest yields (0.71%-1.04%) were observed at Beni Ouarsous, and lowest yields (0.13%-0.50%) at Beni Khellad. The 42 oil samples were submitted to gas chromatography with flame ionization detector (GC(FID)) analysis on two columns of different polarity, in combination with retention indices; 8 samples were also subjected to GC-mass spectrometry (MS) analysis and 7 samples to ¹³C nuclear magnetic resonance (NMR) analysis following a method developed at the University of Corsica.^15-17

In total, 30 individual components have been identified. In short, α-pinene (35.5%-59.0%) was the major component of 50% of the samples; 1,8-cineole (32.9%-46.5%) was the main component of 30% of the samples, the last 20% displayed similar contents of both compounds. The content of limonene varied drastically from sample to sample (3.2%-19.8%). Linalool ranged between 0.2% and 3.7% with an atypical sample having 12.7%.

Other components present at appreciable contents were α-terpineol (1.3%-4.3%), linalyl acetate (tr-2.8%), α-terpinyl acetate (0.0%-2.0%), geranyl acetate (1.1%-4.8%), and methyl eugenol (1.0%-7.3%). The composition of a representative oil sample, analyzed by GC with retention indices (GC(RI)), GC-MS, and ¹³C NMR, is reported in Table 1.

Table 1

Chemical Composition of a Myrtle Leaf Oil Sample From Tlemcen Province.

	Components^a	RIa^b	RIp^c	%	Identification
1	Isobutyl isobutyrate	899	1087	Tr^d	RI, MS, NMR ^e
2	α-Thujene	922	1014	0.2	RI, MS
3	α-Pinene	931	1014	31.3	RI, MS, NMR
4	β-Pinene	969	1108	0.3	RI, MS, NMR
5	Myrcene	980	1161	0.1	RI, MS
6	Isobutyl 2-methylbutyrate	987	1172	0.1	RI, MS, NMR
7	2-Methylbutyl isobutyrate	996	1194	0.1	RI, MS, NMR
8	δ-3-Carene	1004	1145	0.4	RI, MS, NMR
9	p-Cymene	1011	1268	0.9	RI, MS, NMR
10	Limonene*	1022	1199	11.1	RI, MS, NMR
11	1,8-Cineole*	1022	1209	36.8	RI, MS, NMR
12	(E)-β-Ocimene	1035	1246	0.1	RI, MS
13	γ-Terpinene	1047	1241	0.4	RI, MS, NMR
14	Terpinolene	1077	1279	0.2	RI, MS
15	Linalool	1083	1542	3.6	RI, MS, NMR
16	2-Methylbutyl 2-methylbutyrate	1089	1276	0.4	RI, MS, NMR
17	trans-Pinocarveol	1122	1649	0.3	RI, MS, NMR
18	Terpinen-4-ol	1160	1596	0.3	RI, MS, NMR
19	α-Terpineol	1171	1691	3.6	RI, MS, NMR
20	Geraniol	1232	1841	0.5	RI, MS, NMR
21	Linalyl acetate	1238	1553	Tr	RI, MS, NMR
22	α-Terpinyl acetate	1330	1691	Tr	RI, MS, NMR
23	Geranyl acetate	1359	1752	2.9	RI, MS, NMR
24	Methyl eugenol	1368	2008	2.2	RI, MS, NMR
25	(E)-β-Caryophyllene	1413	1588	0.1	RI, MS, NMR
26	α-Humulene	1446	1660	0.1	RI, MS, NMR
27	Dione^f	1490	2033	0.5	RI, NMR
28	Spathulenol	1559	2113	0.1	RI, MS, NMR
29	Caryophyllene oxide	1565	1971	0.1	RI, MS, NMR
30	Humulene oxide	1589	2028	0.1	RI, MS, NMR
	Total (%)			96.8

^aComponents are listed following their order of elution on apolar chromatographic column, (BP-1), percentages on apolar column except for compounds with * (BP-20).

^bRIa, retention indices on apolar column.

^cRIp, retention indices on polar column.

^dTr, trace.

^e NMR (italic), compound identified by NMR in another oil sample.

^fDione, 3,3,5,5,8,8-hexamethyl-7-oxabicyclo[4.3.0]non-1(6)-ene-2,4-dione.

The 42 compositions were subjected to statistical analysis in order to distinguish clusters. The combination of hierarchical cluster analysis (HCA; dendrogram shown in Figure 1) and principal component analysis (PCA; plot shown in Figure 2), for which the first two axes accounted for 70.4% and 20.3% of the total variance of the population, respectively, suggested the existence of two principal groups, group II being subdivided into two subgroups IIA and IIB. Groups and subgroups were differentiated by their content of α-pinene and 1,8-cineole; however, limonene and methyl eugenol contributed to the differentiation (Table 2).

Figure 1

Dendrogram obtained by hierarchical cluster analysis of the 42 samples of myrtle essential oil from northwestern Algeria.

Figure 2

Principal component analysis of the 42 samples of Myrtus communis essential oil from northwestern Algeria.

Table 2

Main Components of 42 Samples of Myrtle Essential Oil From Northwestern Algeria.

	Group I (16 samples)				Group IIA (17 samples)				Group IIB (9 samples)
Components^a	Mean	SD^b	Min^c	Max^c	Mean	SD^b	Min^c	Max^c	Mean	SD^b	Min^c	Max^c
Isobutyl isobutyrate	0.2	0.3	Tr^d	1.2	0.6	0.9	Tr	2.7	0.3	0.6	Tr	2.0
α-Pinene	43.2	7.4	24.9	59.0	35.0	4.7	26.6	42.0	30.6	4.8	24.3	37.1
p-Cymene	0.7	0.6	0.2	2.4	0.7	0.4	0.2	1.5	0.6	0.4	0.2	1.1
Limonene*	13.0	4.5	4.2	19.8	10.6	2.8	3.2	15.9	6.0	1.0	4.5	7.1
1,8-Cineole*	23.7	4.5	13.2	30.0	34.1	2.3	29.9	38.2	42.2	3.6	38.0	49.5
Linalool	1.3	1.1	0.2	3.7	2.3	2.9	0.2	12.7	1.6	0.8	0.8	3.3
2-Methylbutyl 2-methylbutyrate	0.7	0.4	0.1	1.3	0.4	0.3	0.1	1.2	0.4	0.2	0.0	0.7
α-Terpineol	2.1	0.7	1.3	3.6	3.1	0.6	2.0	3.9	3.3	0.7	2.5	4.3
Linalyl acetate	0.6	0.8	0.1	2.8	0.7	0.6	0.1	2.0	0.4	0.4	Tr	1.2
α-Terpinyl acetate	0.6	0.4	0.0	1.7	0.7	0.6	0.0	2.0	0.7	0.7	0.0	1.5
Geranyl acetate	2.7	0.9	1.1	4.8	2.6	0.6	1.8	4.0	3.1	0.6	2.1	4.0
Methyl eugenol	2.6	1.4	1.1	7.3	1.7	0.4	1.0	2.4	3.2	2.0	1.0	6.2
Dione^e	0.5	0.2	0.3	0.8	0.4	0.2	0.1	1.1	0.3	0.2	0.1	0.6

^aComponents are listed following their order of elution on apolar chromatographic column, (BP-1), percentages on apolar column except for compounds with an asterisk, percentages on polar column (BP-20).

^bSD, standard deviation.

^c Min, minimum; Max, maximum.

^dTr, trace.

^eDione, 3,3,5,5,8,8-hexamethyl-7-oxabicyclo[4.3.0]non-1(6)-ene-2,4-dione.

The composition of samples of Group I (16/42 samples) was dominated by α-pinene (mean M = 43.2%, standard deviation SD = 7.4); the content of that compound reached 59.0% in one sample. Other components present at appreciable contents were 1,8-cineole (M = 23.7%, SD = 4.5) and limonene (M = 13.0%, SD = 4.5). Samples of subgroups IIA (17/42 samples) and IIB (9/42 samples) contained similar amounts of α-pinene (M = 35.0%, SD = 4.7 and M = 30.6%, SD = 4.8, respectively). They were differentiated by their contents of 1,8-cineole, more abundant in subgroup IIB (M = 42.2%, SD = 3.6 vs M = 34.1%, SD = 2.3) and limonene more abundant in subgroup IIA (M = 10.6%, SD = 2.8 vs M = 6.0%, SD = 1.0).

The compositions of α-pinene-rich oil samples of Group I isolated from plants harvested in northwestern Algeria resembled to those of samples of subroups IA and IB coming from plants growing wild in northeastern and central-north Algeria.⁷ Samples of subgroup IIA containing about one-third of α-pinene as previously observed for some samples from eastern and central Algeria differed however by higher contents of 1,8-cineole or limonene. Finally, samples of subgroup IIB (9/42 samples) displayed a high percentage of 1,8-cineole that brought a slight difference with all other Algerian myrtle oil samples.

In conclusion, the composition of essential oils isolated from myrtle leaves harvested in northwestern Algeria completes previous results relative to the same plant growing wild in north-eastern and central-north Algeria and provides an overview of Algerian myrtle leaf oil. Although quantitative differences have been observed in the content of the main components, it could be assumed that Algerian myrtle leaf oil belongs to the “α-pinene-1,8-cineole” chemotype, also found in Tunisia,^13,18,19 Sardinia,²⁰ and Corsica.^21,22 In contrary, Algerian myrtle leaf oil differs drastically from oils containing myrtenyl acetate at appreciable content isolated from leaves harvested in Morocco,^12-14 Spain,²³ Portugal,²⁴ Italy,²⁵ ex-Yugoslavia,¹³ Croatia,²⁶ Albania,²⁷ and Turkey.²⁸

The occurrence of the “α-pinene-1,8-cineole” chemotype of myrtle leaf oil from eastern to northwestern Algeria could be advantageous for its production, commercialization, and eventual exportation. Indeed, it has been demonstrated in vitro that Algerian myrtle leaf oil (“α-pinene-1,8-cineole” chemotype) displays various biological activities: antibacterial activity,^8,10,29,30 antifungal effect, anti-inflammatory potential (myrtle leaf oil was able to significantly inhibit nitric oxide production, without affecting cell viability),⁴ antioxidant activity,³¹ antimotility, and antidiarrheal activity.³² Biological activity was also assessed on a pest destructive of stored products, Callosobruchus maculatus.³³ Therefore, a homogenous oil composition is obviously appreciated.

Experimental

Plant Material

Leaves of M. communis were collected in February-March 2018 from northwestern Algeria. Forty-two samples of individual plants were harvested in 5 stations at Tlemcen Province (Figure 3): Beni Khellad (BK1-9), Honaïne (H1-9), Beni Ouarsous (BO1-8), Nedroma (N1-7), and Aïn Kebira (AK1-9).

Figure 3

Harvesting locations of the leaves of Myrtus communis.

Identification of the plants was performed by Pr N. Benabadji (Laboratory of Ecology and Management of Natural Ecosystems, Tlemcen University). A voucher specimen has been deposited at the Laboratory of Natural Products, (Tlemcen University), under the accession number M. 1856.

Isolation of Essential Oils

The EOs were obtained from each sample by hydrodistillation of dried leaves (160-180 g) in a Clevenger-type apparatus for 2.5 hours. Essential oil yields were estimated on the basis of the dry weight of plant material.

GC (FID) and ¹³C-NMR Analyses

Analyses have been carried out as previously reported.^6,34

GC-MS Analysis

The EOs were analyzed with a Perkin-Elmer TurboMass detector (quadrupole), directly coupled to a Perkin-Elmer Autosystem equipped with a fused-silica capillary column (50 m × 0.22 mm i.d., film thickness 0.25 µm), BP-1 (dimethylpolysiloxane). Carrier gas, helium at 0.8 mL/min; split: 1/60; injection volume: 0.5 µL; injector temperature: 250°C; oven temperature programmed from 60 °C to 220 °C at 2 °C/min and then held isothermal (20 min); ion source temperature, 250°C; energy ionization, 70 eV; electron ionization mass spectra were acquired over the mass range 40 to 400 Da.

Identification of Components

As previously reported.^6,34

Data Analysis

PCA and HCA (Ward’s method) were performed with Xlstat (Adinsoft, France).³⁵

Footnotes

Acknowledgments

This work was conducted within the cooperative program between the Universities of Abou Bekr Belkaïd (Tlemcen) and Pascal Paoli (Corsica).

Declaration of Conflicting Interests

The author(s) declared no potential conflicts of interest with respect to the research, authorship, and/or publication of this article.

Funding

The author(s) disclosed receipt of the following financial support for the research, authorship, and/or publication of this article: C. Bekhechi thanks University of Corsica for a research grant as associate professor (September 2018).

References

Bessah

Benyoussef

E-H

. La filière des huiles essentielles Etat de l’art, impacts et enjeux socioéconomiques. Rev Energies Renouvelables. 2015;18(3):513-528.

Sahi

. La dynamique des plantes aromatiques et médicinales en Algérie. In: Ilbert

Hoxha

Sahi

Courivaud

Chailan

, eds. Le Marché Des Plantes Aromatiques et Médicinales: Analyse des Tendances du Marché Mondial et des Stratégies Economiques en Albanie et en Algérie. Montpellier: CIHEAM / France AgriMer; 2016:101-140.

Escriva

. Les Huiles Essentielles Corses. Brussels, Belgium: Amyris; 2012.

Bouzabata

Cabral

Gonçalves

et al . Myrtus communis L. as source of a bioactive and safe essential oil. Food Chem Toxicol. 2015;75:166-172.doi:10.1016/j.fct.2014.11.009

Bouzabata

Casanova

Bighelli

Cavaleiro

Salgueiro

Tomi

. The genus Myrtus L. in Algeria: composition and biological aspects of essential oils from M. communis and M. nivellei: a review. Chem Biodivers. 2016;13(6):672-680.doi:10.1002/cbdv.201500342

Bouzabata

Boussaha

Casanova

Tomi

. Composition and chemical variability of leaf oil of Myrtus communis from North-Eastern Algeria. Nat Prod Commun. 2010;5(10):1662-1689.doi:10.1177/1934578X1000501029

Bouzabata

Castola

Bighelli

Abed

Casanova

Tomi

. Chemical variability of Algerian Myrtus communis L. Chem Biodivers. 2013;10(1):129-137.doi:10.1002/cbdv.201200150

Hennia

Miguel

MG.

Brada

Nemmiche

Figueiredo

. Composition, chemical variability and effect of distillation time on leaf and fruits essential oils of Myrtus communis from North Western Algeria. J Essent Oil Res. 2016;28(2):146-156.doi:10.1080/10412905.2015.1090936

Hennia

Brada

Nemmiche

Fauconnier

M-L.

Lognay

. Chemical composition and antibacterial activitiy of the essential oils of Algerian Myrtus communis L. J Essent Oil Res. 2015;27(4):324-328.doi:10.1080/10412905.2015.1023905

10.

Djenane

Yangüela

Amrouche

Boubrit

Boussad

Roncalés

. Chemical composition and antimicrobial effects of essential oils of Eucalyptus globulus, Myrtus communis and Satureja hortensis against Escherichia coli O157:H7 and Staphylococcus aureus in minced beef. Food Sci Technol Int. 2011;17(6):505-515.doi:10.1177/1082013211398803

11.

Touaibia

. Composition and anti-inflammatory effect of the common myrtle (Myrtus communis L.) essential oil growing wild in Algeria. Phytothérapie. 2017.doi:10.1007/s10298-017-1100-9

12.

Gauthier

Gourai

Bellakhdar

. A propos de l'huile essentielle de Myrtus communis L. var. italica récolté Au Maroc. I. Rendements et compositions durant un cycle végétatif annuel. Al Biruniya Revue Marocaine Pharm. 1988;4(2):97-116.

13.

Chalchat

J-C.

Garry

R-P.

Michet

. Essential oils of Myrtle (Myrtus communis L.) of the Mediterranean littoral. J Essent Oil Res. 1998;10(6):613-617.doi:10.1080/10412905.1998.9700988

14.

Fadil

Farah

Ihssane

Haloui

Lebrazi

Rachiq

. Intrapopulation variability of Myrtus communis L. growing in Morocco: Chemometric investigation and antibacterial activity. J Appl Res Med Aromat Plants. 2017;7:35-40.doi:10.1016/j.jarmap.2017.04.006

15.

Tomi

Bradesi

Bighelli

Casanova

. Computer-aided identification of individual components of essential oils using carbon-13 NMR spectroscopy. J Magn Reson Anal. 1995;1(1):25-34.

16.

Tomi

Casanova

. ¹³C NMR as a tool for identification of individual components of essential oils from Labiatae-A review. Acta Hortic. 2006;723 185-192.doi:10.17660/ActaHortic.2006.723.21

17.

Ouattara

ZA.

Boti

JB.

Ahibo

et al . The key role of ¹³CNMR analysis in the identification of individual components of Polyalthia longifolia leaf oil. Flavour Fragr J. 2014;29(6):371-379.doi:10.1002/ffj.3215

18.

Wannes

WA.

Mhamdi

Sriti

Marzouk

. Changes in essential oil composition of Tunisian Myrtus communis var. italica L. during its vegetative cycle. J Essent Oil Res. 2010;22(1):13-18.doi:10.1080/10412905.2010.9700256

19.

Messaoud

Zaouali

Salah

AB.

Khoudja

ML.

Boussaid

. Myrtus communis in Tunisia: variability of the essential oil composition in natural populations. Flavour Fragr. J.. 2005;20(6):577-582.doi:10.1002/ffj.1490

20.

Tuberoso

CIG.

Barra

Angioni

Sarritzu

Pirisi

. Chemical composition of volatiles in sardinian myrtle (Myrtus communis L.) alcoholic extracts and essential oils. J Agric Food Chem. 2006;54(4):1420-1426.doi:10.1021/jf052425g

21.

Bradesi

Tomi

Casanova

Costa

Bernardini

. Chemical composition of myrtle leaf essential oil from Corsica (France). J Essent Oil Res. 1997;9(3):283-288.doi:10.1080/10412905.1997.10554245

22.

Bazzali

Tomi

Casanova

Bighelli

. Occurrence of C8-C10 esters in Mediterranean Myrtus communis L. leaf essential oil. Flavour Fragr. J. 2012;27(5):335-340.doi:10.1002/ffj.3102

23.

Boelens

MH.

Jimenez

. The chemical composition of Spanish myrtle leaf oils. Part I. J Essent Oil Res. 1991;3(3):173-177.doi:10.1080/10412905.1991.9700498

24.

Pereira

Cebola

M-J.

Bernardo-Gil

. Evolution of the yields and composition of essential oil from Portuguese myrtle (Myrtus communis L.) through the vegetative cycle. Molecules. 2009;14(8):3094-3105.doi:10.3390/molecules14083094

25.

De Laurentis

Rosato

Gallo

Leone

Milillo

. Chemical composition and antimicrobial activity of Myrtus communis . Rivista Italiana EPPOS. 2005;39(giugno):3-8.

26.

Jerković

Radonić

Borčić

. Comparative study of leaf, fruit and flower essential oils of Croatian Myrtus communis L. during a one-year vegetative cycle. J Essent Oil Res. 2002;14(4):266-270.doi:10.1080/10412905.2002.9699849

27.

Asllani

. Chemical composition of Albanian myrtle oil (Myrtus communis L. J Essent Oil Res. 2000;12(2):140-142.doi:10.1080/10412905.2000.9699481

28.

Özek

Demirci

Baser

KHC

. Chemical composition of Turkish myrtle oil. J Essent Oil Res. 2000;12(5):541-544.doi:10.1080/10412905.2000.9712154

29.

Ben Ghnaya

Chograni

Messoud

Boussaid

. Comparative chemical composition and antibacterial activities of Myrtus communis L. essential oils isolated from Tunisian and Algerian Population. J. Plant Pathol Microbiol. 2013;4(7):186.

30.

Badra

Saoudi

Akila

. Essential oil chemical composition of myrtle growing in Northeastern Algeria and estimation of its antibacterial effectiveness. Am J Biochem Biotechnol. 2016;12(2):110-121.doi:10.3844/ajbbsp.2016.110.121

31.

Belmimoun

Meddah

AT.

Sonnet

. Antibacterial and antioxidant activities of the essential oils and phenolic extracts of Myrtus communis and Zygophylum album from Algeria. J Fundam App Sci. 2016;8(2):510-524.doi:10.4314/jfas.v8i2.22

32.

Benchikh

Benabdallah

Dahamna

Khennouf

Flamini

Amira

. Antimotility and andidiarrhoel activity of Myrtus communis L. leaves essential oil in mice. Int J Pharmacogn Phytochem Res. 2016;8(7):1238-1244.

33.

Toudert-Taleb

Hedjal-Chebheb

Hami

Debras

J-F.

Kellouche

. Composition of essential oils extracted from six aromatic plants of kabylian origin (Algeria) and evaluation of their bioactivity on Callosobruchus maculatus (Fabricius, 1775) (Coleoptera: Bruchidae). Afr Entomol. 2014;22(2):417-427.doi:10.4001/003.022.0220

34.

Djié Bi

TMG.

Yapi

AT.

Ouattara

et al . Composition and variability of leaf oil from Monodora crispata ¹³C NMR identification of heat-sensitive compounds. Nat Prod Commun. 2017;12(3):435-438.doi:10.1177/1934578X1701200332

35.

Legendre

. Numerical Ecology. 2nd edn. Amsterdam: Elsevier Science; 1998.

Composition and Chemical Variability of Myrtus communis Leaf Oil From Northwestern Algeria

Abstract

Keywords

Experimental

Plant Material

Isolation of Essential Oils

GC (FID) and 13C-NMR Analyses

GC-MS Analysis

Identification of Components

Data Analysis

Footnotes

Acknowledgments

Declaration of Conflicting Interests

Funding

References

GC (FID) and ¹³C-NMR Analyses