Phytochemical investigation of the ethyl acetate soluble fraction from the leaves of Epigynum cochinchinensis led to the isolation of one new C21 pregnane glycoside, epigycoside C (1), along with two known analogues epigycoside A (2) and epigynoside G (3). Their structures were elucidated on the basis of extensive spectroscopic techniques. The in vitro immunosuppressive activity of compound 1 was evaluated against concanavalin A (Con A)- and lipopolysaccharides (LPS)-stimulated proliferation of mice splenocytes. Compound 1 displayed significant immunosuppressive activities in a dose-dependent manner.
LiZ., ChenG., LuX., WangH., FengB., PeiY. (2013) Three new steroid glycosides from the starfish Asterina pectinifera. Natural Product Research, 27, 1816–1822.
2.
GaoF., YaoY.C., CaiS.B., ZhaoT.R., YangX.Y., FanJ., LiX.N., CaoJ.X., ChengG.G. (2017) Novel immunosuppressive pregnane glycosides from the leaves of Epigynum auritum. Fitoterapia, 118, 107–111.
3.
JeongH.Y., SungG.H., KimJ.H., YoonJ.Y., YangY., ParkJ.G., KimS.H., YiY.S., YangW.S., YoonD.H. (2014) Syk and Src are major pharmacological targets of a Cerbera manghas methanol extract with kaempferol-based anti-inflammatory activity. Journal of Ethnopharmacology, 151, 960–969.
4.
LiJ.L., GaoZ.B., ZhaoW.M. (2015) Identification and evaluation of antiepileptic activity of C21 steroidal glycosides from the roots of Cynanchum wilfordii. Journal of Natural Products, 79, 89–97.
MiddletonD.J. (2005) A revision of Epigynum (Apocynaceae: Apocynoideae). Harvard Papers in Botany, 10, 67–81.
9.
WanZ., YaoY.C., GaoF., CaiS.B., AsfarK., ZhaoT.R., YangX.Y., FanJ., QianS.Y., CaoJ.X. (2017) A new immunosuppressive pregnane glycoside from aqueous fraction of Epigynum cochinchinensis. Natural Product Research, 31, 2893–2899.
10.
DengA.J., YuJ.Q., LiZ.H., MaL., ZhangZ.H., QinH.L. (2017) 14,15-Secopregnane-type glycosides with 5α:9α-peroxy and Δ6,8(14)-diene linkages from the roots of Cynanchum stauntonii. Molecules, 22, 860–867.
11.
SteinbergK.M., ShresthaS., DosokyN.S., MonzoteL., PiñónA., HaberW.A., SetzerW.N. (2017) Cytotoxic and antileishmanial components from the bark extract of Ruyschia phylladenia from Monteverde, Costa Rica. Natural Product Communications, 12, 1–2.
12.
LiX., XuanB., ShouQ., ShenZ. (2014) New flavonoids from Campylotropis hirtella with immunosuppressive activity. Fitoterapia, 95, 220–228.