Two glucans, one α-glucan (TM-I) and one β-glucan (TM-II) were isolated from an edible mushroom, Termitomyces microcarpus by extraction with 5% NaOH/0.05% NaBH4, followed by a precipitation with 1 M acetic acid. The repeating units of two polysaccharides have been identified by means of NMR studies (1H, 13C, DEPT-135 and HSQC) and chemical investigations. The structures of repeating units of two polymers were established as
MizunoM, MorimotoM, MinatoK, TsuchidaH. (1998) Polysaccharides from Agaricus blazei stimulate lymphocyte T-cell subsets in mice. Journal of Bioscience Biotechnology and Biochemistry, 62, 434–437.
2.
JiskaniMM. (2001) Energy potential of mushrooms. The Economic Business Review, P IV, Oct 15–21.
3.
BelloBK, AkinyeleBJ. (2007) Effect of fermentation on the microbiology and mineral composition of an edible mushroom Termitomyces robustus (Fries). International Journal of Biological Chemistry, 1, 237–243.
4.
MattilaP, KönköK, EurolaM, PihlavaJM, AstolaJ, VahteristoL, HietaniemiV, KumpulainenJ, ValtonenM, PiironenV. (2001) Contents of vitamins, mineral elements, and some phenolic compounds in cultivated mushrooms. Journal of Agricultural Food Chemistry, 49, 2343–2348.
5.
ChiharaG, MaedaY, HamuroJ, SasakiT, FukuokaF. (1969) Inhibition of mouse sarcoma 180 by polysaccharides from Lentinus edodes. Nature, 222, 687–696.
6.
MaedaYY, ChiharaG. (1971) Lentinan, a new immune-accelerator of cell-mediated responses. Nature, 229, 634–638.
7.
SasakiT, TakasukaN. (1976) Further study of the structure of lentinan, an anti-tumor polysaccharide from Lentinus edodes. Carbohydrate Research, 47, 99–105.
8.
MizunoT, HagiwaraT, NakamuraT, ItoH, ShimuraK, SumiyaT, AsakuraA. (1990) Studies on the best-mediated antitumor polysaccharides. Part XIII, antitumor activity and some properties of water-soluble polysaccharides from himemat-sutake, the fruiting bodies of Agaricus blazei Murill. Agricultural and Biological Chemistry, 54, 2897–2950.
9.
TanigamiY, KusumotoS, NagaoS, KokeguchiS, KatoK, KotaniS, ShibaT. (1991) Partial degradation and biological activities of an antitumor polysaccharide from rice bran. Chemical and Pharmaceutical Bulletin, 39, 1782–1787.
ChandraK, GhoshK, OjhaAK, IslamSS. (2009) A protein containing glucan from an edible mushroom, Termitomyces microcarpus (var). Natural Product Communications, 4, 553–556.
12.
ShidaM, UchidaT, MatsudaK. (1978) A (1→3)-α-D-glucan isolated from the fruit bodies of Lentinus edodes. Carbohydrate Research, 60, 117–127.
13.
ChandraK, GhoshK, RoySK, MondalS, MaitiD, OjhaAK, DasD, MondalS, IslamSS. (2007) A water-soluble glucan isolated from an edible mushroom Termitomyces microcarpus. Carbohydrate Research, 342, 2484–2489.
14.
BhanjaSK, RoutD, PatraP, SenIK, NandanCK, IslamSS. (2014) Water-insoluble glucans from the edible fungus Ramaria botrytis. Bioactive Carbohydrates and Dietary Fibre, 3, 52–58.
15.
CiucanuI, KerekF. (1984) A simple and rapid method for the permethylation of carbohydrates. Carbohydrate Research, 131, 209–217.
16.
AgrawalPK. (1992) NMR spectroscopy in the structural elucidation of oligosaccharides and glycosides. Phytochemistry, 31, 3307–3330.
17.
CarboneroER, GracherAHP, SmiderleFR, RosadoFR, SassakiGL, GorinPAJ, IacominiM. (2006) A β-glucan from the fruit bodies of edible mushrooms Pleurotus eryngii, and Pleurotus ostreatoroseus. Carbohydrate Polymer, 66, 252–257.
18.
SmiderleFR, OlsenLM, CarboneroER, BaggioCH, FreitasCS, MarconR, SantosARS, GorinPAJ, IacominiM. (2008) Anti-inflammatory and analgesic properties in a rodent model of a (1→3), (1→6)-linked β-glucan isolated from Pleurotus pulmonarius. European Journal of Pharmacology, 597, 86–91.
19.
BhanjaSK, NandanCK, MandalS, BhuniaB, MaitiTK, MondalS, IslamSS. (2012) Isolation and characterization of the immunostimulating β-glucans of an edible mushroom, Termitomyces robustus (var). Carbohydrate Research, 357, 83–89.
