Abstract
To investigate the effect of insect damage on the biosynthesis of secondary metabolites, we cultivated Angelica acutiloba with and without damage caused by the larvae of Papilio machaon. Compounds from the leaves and roots of A acutiloba were extracted with chloroform and analyzed by GC-MS. We annotated the peaks based on the mass spectral data and retention times. In addition, the effects of insect damage on the plants were investigated by principal component analysis (PCA). As a result, it was clarified that the amounts of ligustilide, γ-terpinene and β-caryophyllene, increased in leaves after being damaged by insects. Polyacetylenes also increased in the roots of damaged plants. In addition, as prompt responses, increases in the proteins relating to hydrogen peroxide synthesis and decreases in the proteins concerned with a non-urgent response to pathogenic attack were clarified by proteomic analysis. These results indicate that cultivation methods using the chemical-ecological response of the plant can contribute to the production of higher-quality crude drugs derived from A. acutiloba.