Essential oils and volatile emission from leaves and flowers of three Helichrysum species (H. arenarium, H. nudifolium, H. graveolens) were analysed by GC-MS and SPME analysis. A total of 112 components were identified in the EOs representing 92.3–99.9% of the whole oil composition, while 109 constituents were identified in the spontaneous volatile emission accounted for 78.7–98.8% of the total volatile organic compounds (VOCs). Sesquiterpenes represented the main class of constituents in the EOs of the majority studied species. β-caryophyllene was the key compound of H. arenarium and H. nudifolium leaves, even though (E)-2-hexenal showed high percentage in H. arenarium leaves. H. graveolens EO showed a good amount of diterpenes (cembrene and beyerene), absent in the other species. SPME analyses were carried out for the first time to complete the chemotaxonomic investigation on the volatile organic constituents of these plants.
AngioniA, BarraA, ArlorioM, CoissonJD, RussoMT, PirisiFM, SattaM, CabrasP. (2003) Chemical composition, plant genetic differences, and antifungal activity of the essential oil of Helichrysum italicum G. Don ssp. microphyllum (Willd) Nym. Journal of Agricultural and Food Chemistry, 51, 1030–1034.
2.
LourensACU, ReddyD, BaserKHC, ViljoenAM, Van VuurenSF. (2004) In vitro biological activity and essential oil composition of four indigenous South African Helichrysum species. Journal of Ethnopharmacology, 95, 253–258
3.
LourensACU, ViljoenAM, HeerdenFR. (2008) South African Helichrysum species: a review of the traditional uses, biological activity and phytochemistry. Journal of Ethnopharmacology, 119, 630–652
4.
LourensACU, Van VuurenSF, ViljoenAM, DavidsH, Van HeerdenFR. (2011) Antimicrobial activity and in vivo cytotoxicity of selected South African Helichrysum species. South African Journal of Botany, 77, 229–235.
5.
Moroni-FortunatoI, MontemurroC, RutaC, PerrriniR, SabettaW, BlancoA. (2010) Essential oils, genetic relationships and in vitro establishment of Helichrysum italicum (Roth) G. Don spp. italicum from wild Mediterranean germplasm. Industrial Crops and Products, 32, 639–649.
6.
UsaiM, FoddaiM, BernardiniAF, MuselliA, CostaJ, MarchettiM. (2010) Chemical composition and variation of the essential of wild Sardinian Helichrysum italicum G. Don subsp. Microphyllum (Willd.) Nym from vegetative period to post-blooming. Journal of Essential Oil Research, 22, 373–380.
7.
PreedyVR. (2015) Essential Oils in Food Preservation, Flavor and Safety. Academic press, King's College London, London, UK.
8.
ViegasA, Palmeira de OliveiraA, SalgueiroL, Martinez de OliveiraJ, Palmeira de OliveiraR. (2014) Helichrysum italicum from traditional use to scientific data. Journal of Ethnopharmacology, 151, 54–65.
9.
JudzentieneA, ButkieneR. (2006) Chemical composition of the essential oils of wild Helichrysum arenarium (L.) with differently coloured inflorescences from Eastern Lithuania. Journal of Essential Oil Research, 18, 80–83.
10.
CzinnerE, LemberkovicsE, Bihátsi-KarsaiE, VitányiG, LelikL. (2000) Composition of the essential oil of Helichrysum arenarium (L.) Moench. Journal of Essential Oil Research, 12, 728–730.
11.
JägerAK, HutchingsA, Van StadenJ. (1996) Screening of Zulu medicinal plants for prostaglandina-synthesis inhibitors. Journal of Ethnopharmacology, 52, 95–100.
12.
ErogluHE, HamzaogluE, AksoyA, BudakU, AlbayrakS. (2010) Cytogenetic effects of Helichrysum arenarium in human lymphocytes cultures. Turkish Journal of Biology, 34, 253–259.
13.
SkakunNP, StepanovNYu. (1988) Comparative evaluation of the hepatoprotective, antioxidant and choleretic activity of flavonoid drugs. Vracebnoe Delo, 12, 52–54.
14.
StaffordGI, JägerAK, Van StadenJ. (2005) Activity of traditional South African sedative and potentially CNS-acting plants in the GABA-benzodiazepine receptor assay. Journal of Ethnopharmacology, 100, 210–215.
15.
JarzyckaA, LewińskaA, GancarzR, WilkKA. (2013) Assessment of extracts of Helichrysum arenarium, Crataegus monogyna, Sambucus nigra in photoprotective UVA and UVB; photostability in cosmetic emulsions. Journal of Photochemistry and Photobiology B: Biology, 128, 50–57.
16.
HilliardOM. (1983) Gnaphaliinae parte 1. In: LeistnerOA. Flora of Southern Africa. Botanical Research Institute, Pretoria, 1–233.
17.
CervelliC. (2016) Il fiore del sole: aspetti biologici e applicative delle specie di Helichrysum. Casabianca, Sanremo (Italy).
18.
KomarovVL; SchischkinBK; ShetlerSG; FetGN. (1999) Flora of the USSR, Compositae, Tribes Eupatorieae, Astereae, Inuleae, Ambrosieae, Heliantheae and Helenieae. Washington, D.C.Amerind Publishing.
19.
SezikE, TabataM, YesiladaE, HondaG, GotoK, IkeshiroY. (1991) Traditional medicine in Turkey I. Folk medicine in Northeast Anatolia. Journal of Ethnopharmacology, 35, 191–196.
20.
YesiladaE, HondaG, SezikE, TabataM, FujitaT, TanakaT, TakedaY, TakaishiY. (1995) Traditional use in Turkey. V. Folk medicine in the Taurus Mountains. Journal of Ethnopharmacology, 46, 133–152.
21.
AslanM., OrhanDD, OrhanN, SezikE, YesiladaE. (2007) A study of antidiabetic and antioxidant effects of Helichrysum graveolens capitulums in streptozotocin-induced diabetic rats. Journal of Medicinal Food, 10, 396–400.
22.
SüntarI, Küpeli AkkolE, KelesH, YesiladaE, SarkerSD. (2013) Exploration of the wound healing potential of Helichrysum graveolens (Bieb.) Sweet: isolation of apigenin as an active component. Journal of Ethnopharmacology, 149, 103–110.
23.
OnaranM, OrhanN, FarahvashA, EkinHN, KocabιyιkM, GönülİI, Şenİ, AslanM. (2016) Successful treatment of sodium oxalate induced urolithiasis with Helichrysum flowers. Journal of Ethnopharmacology, 186, 322–328.
24.
MaggioA, BrunoM, GuarinoR, SenatoreF, IlardiV. (2016) Contribution to a taxonomic revision of the Sicilian Helichrysum taxa by PCA analysis of their essential-oil compositions. Chemistry & Biodiversity, 13, 151–159.
25.
RoussisV, TsoukatouM, PetrakisPV, ChinouI, SkoulaM, HarborneJ. (2000) Volatile constituents of four Helichrysum species growing in Greece. Biochemical Systematic and Ecology, 28, 163–175.
26.
OrnanoL, VendittiA, SannaC, BalleroM, MaggiF, LupidiG, BramucciM, QuassintiL, BiancoA. (2015) Chemical composition and biological activity of the essential oil from Helichrysum microphyllum Cambesss. Ssp. Tyrrhenicum Bacch., Brullo e Giusso growing in La Maddalena Archipelago, Sardinia. Journal of Oleo Science, 64, 19–26.
27.
BagciE, ElkiranO, EvrenH. (2013) Constituents of the essential oils of Helichrysum graveolens (Bieb.) Sweet from Turkey. Asian Journal of Chemistry, 25, 7254–7256.
28.
MaffeiMA, GertschJ, AppendinoG. (2011) Plant volatiles: production, function and pharmacology. Natural Products Reports, 28, 1359–1380.
29.
OjiK, ShafaghatA. (2012) Constituents and antimicrobial activity of the essential oils from flower, leaf and stem of Helichrysum armenium. Natural Product Communications, 7, 671–674.
30.
CavalliJ, RanariveloL, RatsimbasonM, BernardiniA, CasanovaJ. (2001) Constituents of the essential oil of six Helichrysum species from Madagascar. Flavour and Fragrance Journal, 16, 253–256.
31.
LeonardiM, AmbryszewskaKE, MelaiB, FlaminiG, CioniPL, ParriF, PistelliL. (2013) Essential oil composition of Helichrysum italicum (Roth) G. Don ssp. Italicum from Elba Island (Tuscany, Italy). Chemistry & Biodiversity, 10, 343–355.
32.
LawalOA, OgunwandeIA, KasaliAA, OpokuAR, OyedejiAO. (2015) Chemical composition, antibacterial and cytotoxic activities of essential oil from the leaves of Helichrysum odoratissimum grown in South Africa. Journal of Essential Oil Bearing Plants, 18, 236–241.
33.
ReidelRVB, MelaiB, CioniPL, FlaminiG, PistelliL.2016. Aroma profile of Rubus ulmifolius flowers and fruits during different ontogenic phases. Plant & Biosystems, 13, 1776–1784.
34.
PistelliL, GiovanelliS, MargariP, ChiappeS. (2016) Considerable effect of dimethylimidazolium dimethylphosphate in cinnamon essential oil extraction by hydrodistillation. RSC Advances, 6, 52421–52426.
35.
AscrizziR, CioniPL, GiustiG, PistelliL, FlaminiG. (2016) Patterns in volatile emission of different aerial parts of Caper (Capparis spinosa). Chemistry & Biodiversity, 13, 904–912.
