New Steroidal Glycoside and Flavonoid Constituents from Ophiopogon japonicus

Abstract

Chromatographic separations of a methanolic extract of Ophiopogon japonicus tubers resulted in the isolation of a new steroidal glycoside, (25R)-ruscogenin 1-O-(4-O-sulfo)-β-D-fucopyranoside (1), a new C-methylflavonol, 3,4′-dimethoxy-3′,5,5′,7-tetrahydroxy-8-methylflavone (2), and a known flavonol, 3,4′-dimethoxy-3′,5,5′,7-tetrahydroxyflavone (3). The C-methylflavonol was reported for the first time from the genus Ophiopogon. Compound 1 showed moderate cytotoxicity against SK-Mel-2 and KB cells with IC₅₀ values of 24.3 and 28.8 μM, respectively. Compound 2 was active on SK-Mel-2 cells (IC₅₀ 20.3 μM).