The concentration of trigonelline in dry seeds of fenugreek (Trigonella foenum-graecum) was 29±3 μmol/g fresh weight. Trigonelline occurred in cotyledons and embryonic axes of the seedlings, but it was found mainly in the above-ground parts of the young fenugreek plants. 15NH4+-feeding experiments suggest that the de novo biosynthesis of trigonelline from NH4+ occurs mainly in roots. Trigonelline, which is formed in roots using inorganic nitrogen, is transported to the stems and accumulates in leaves.
AshiharaH, LudwigI, KatahiraR, YokotaT, FujimuraT, CrozierA. (2015) Trigonelline and related nicotinic acid metabolites: occurrence, biosynthesis, taxonomic considerations, and their roles in planta and in human health. Phytochemistry Reviews, 14, 765-798.
2.
KatohA, HashimotoT. (2004) Molecular biology of pyridine nucleotide and nicotine biosynthesis. Frontier in Bioscience, 9, 1577-1586.
3.
ZandiP, BasuSK, KhatibaniLB, BalogunMO, AremuMO, SharmaM, KumarA, SenguptaR, LiX, LiY, TashiS, HediA, Cetzal-IxW. (2014) Fenugreek (Trigonella foenum-graecum L.) seed: a review of physiological and biochemical properties and their genetic improvement. Acta Physiologiae Plantarum, 37, 1-14.
4.
JohnsE. (1885) Ueber die Alkaloide des Bockshornsamens. Berichte der Deutschen Chemischen Gesellschaft, 18, 2518-2523.
5.
MatsuiA, YinY, YamanakaK, IwasakiM, AshiharaH. (2007) Metabolic fate of nicotinamide in higher plants. Physiologia Plantarum, 131, 191-200.
6.
ZhengXQ, HayashibeE, AshiharaH. (2005) Changes in trigonelline (N-methylnicotinic acid) content and nicotinic acid metabolism during germination of mungbean (Phaseolus aureus) seeds. Journal of Experimental Botany, 56, 1615-1623.
7.
YinY, MatsuiA, SakutaM, AshiharaH. (2008) Changes in pyridine metabolism profile during growth of trigonelline-forming Lotus japonicus cell cultures. Phytochemistry, 69, 2891-2898.
8.
AshiharaH, YinY, KatahiraR, WatanabeS, MimuraT, SasamotoH. (2012) Comparison of the formation of nicotinic acid conjugates in leaves of different plant species. Plant Physiology and Biochemistry, 60, 190-195.
9.
SasamotoH, AshiharaH. (2014) Effect of nicotinic acid, nicotinamide and trigonelline on the proliferation of lettuce cells derived from protoplasts. Phytochemistry Letters, 7, 38-41.
10.
AshiharaH, WatanabeS. (2014) Accumulation and function of trigonelline in non-leguminous plants. Natural Product Communications, 9, 795-798.
11.
DengWW, AshiharaH. (2015) Occurrence and de novo biosynthesis of caffeine and theanine in seedlings of tea (Camellia sinensis). Natural Product Communications, 10, 703-706.
12.
ShojiT, HashimotoT. (2011) Nicotine biosynthesis. In Plant Metabolism and Biotechnology, John Wiley & Sons, Ltd, Chichester, 191-216.
13.
MurashigeT, SkoogF. (1962) A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiologia Plantarum, 15, 473-497.
14.
DengWW, OgitaS, AshiharaH. (2008) Biosynthesis of theanine (γ-ethylamino-L-glutamic acid) in seedlings of Camellia sinensis. Phytochemistry Letters, 1, 115-119.
