Nineteen compounds, including seven triterpenoids (1–7), five steroids (8–12), four cyclohexenone derivatives (13–16), two benzenoid glycosides (17 and 18) and one lignan (19), were isolated and separated from the leaves of Pandanus utilis through bioactivity-guided fractionation. Among them, one new lanosterol-type triterpenoid was found and named as (24R)-24-methyl-5α-4-demethyllanosta-9(11),25-dien-3β-ol (1). The structures of the isolates were determined by mass and spectroscopic analyses, and the compounds were subjected to anti-inflammatory, anti-oxidative and cytotoxic assays.
VaughanRE, WiehePO. (1953) The genus Pandanus in the Mascarene Islands. Botanical Journal of the Linnean Society of London, 55, 1–33.
2.
ChongHZ, YeapSK, RahmatA, AkimAM, AlitheenNB, OthmanF, Gwendoline-EeCL. (2012) In vitro evaluation of Pandanus amaryllifolius ethanol extract for induction of cell death on non-hormone dependent human breast adenocarcinoma MDA-MB-231 cell via apoptosis. BMC Complementary and Alternative Medicine, 12, 134.
3.
PeungvichaP, ThirawarapanSS, WatanabeH. (1996) Hypoglycemic effect of water extract of the root of Pandanus odorus. Biological and Pharmaceutical Bulletin, 19, 364–366.
4.
PeungvichaP, TemsiririrkkulR, PrasainJK, TezukaY, KadotaS, ThirawarapanSS, WatanabeH. (1998) 4-Hydroxybenzoic acid: a hypoglycemic constituent of aqueous extract of Pandanus odorus root. Journal of Ethnopharmacology, 62, 79–84.
5.
PeungvichaP, ThirawarapanSS, WatanabeH. (1998) Possible mechanism of hypoglycemic effect of 4-hydroxybenzoic acid, a constituent of Pandanus odorus root. The Japanese Journal of Pharmacology, 78, 395–398.
6.
SasidharanS, SumathiV, JegathambigaiNR, LathaLY. (2011) Antihyperglycaemic effects of ethanol extracts of Carica papaya and Pandanus amaryfollius leaf in streptozotocin-induced diabetic mice. Natural Product Research, 25, 1982–1987.
7.
ZhangX, WuC, WuH, ShengL, SuY, ZhangX, LuanH, SunG, SunX, TianY, JiY, GuoP, XuX. (2013) Anti-hyperlipidemic effects and potential mechanisms of action of the caffeoylquinic acid-rich Pandanus tectorius fruit extract in hamsters fed a high fat-diet. PLoS ONE, 8, e61922.
8.
OoiLSM, SunSSM, OoiVEC. (2004) Purification and characterization of a new antiviral protein from the leaves of Pandanus amaryllifolius (Pandanaceae). The International Journal of Biochemistry & Cell Biology, 36, 1440–1446.
9.
TanMA, TakayamaH, AimiN, KitajimaM, FranzblauSG, NonatoMG. (2008) Antitubercular triterpenes and phytosterols from Pandanus tectorius Soland. var. laevis. Journal of Natural Medicines, 62, 232–235.
YanoK, AkihisaT, TamuraT, MatsumotoT. (1992) Four 4a-methylsterols and triterpene alcohols from Neolitsea aciculata. Phytochemistry, 31, 2093–2098.
12.
LiHT, DjerassiC. (1982) Structural and stereochemical aspects of base-catalyzed double bond isomerization of sterols with unsaturated side chains. The Journal of Organic Chemistry, 47, 4298–4303.
13.
InadaA, IkedaY, MurataH, InatomiY, NakanishiT, BhattacharyyaK, KarT, BocelliG, CantoniA. (2005) Unusual cyclolanostanes from leaves of Pandanus boninensis. Phytochemistry, 66, 2729–2733.
14.
MantschHH, SmithICP. (1973) A study of solvent effects on the 13C nuclear magnetic resonance spectra of cholesterol, pyridine, and uridine. Canadian Journal of Chemistry, 51, 1384–1391.
15.
HabibMR, NikkonF, RahmanM, HaqueME, KarimMR. (2007) Isolation of stigmasterol and β-sitosterol from methanolic extract of root bark of Calotropis gigantea (Linn). Pakistan Journal of Biological Sciences, 10, 4174–4176.
16.
GopalakrishnanM, NarayananGS, GrenzM. (1990) Nonsaponifiable lipid constituents of Cardamom. Journal of Agricultural Food Chemistry, 38, 2133–2136;
17.
HoaNT, DienPH, QuangDN. (2014) Cytotoxic steroids from the stem barks of Pandanus tectorius. Research Journal of Phytochemistry, 8, 52–56.
18.
ÖksüzS, UlubelenA, BarlaA. (2002) Terpenoids and aromatic compounds from Euphorbia heteradena. Turkish Journal of Chemistry, 26, 457–463.
19.
SilvaM, VieiraLMM, AlmeidaAP, SilvaAMS, SecaAML, BarretoMC, NetoAI, PedroM, PintoE, KijjoaA. (2013) Chemical study and biological activity evaluation of two Azorean macroalgae: Ulva rigida and Gelidium microdon. Oceanography, 1, 102.
20.
MatsunamiK, OtsukaH, TakedY. (2010) Structural revisions of blumeno C glucoside and byzantionoside B. Chemical and Pharmaceutical Bulletin, 58, 438–441.
21.
WenP, HanH, WangR, WangN, YaoX. (2007) C-glycosylfavones and aromatic glycosides from Campylotropis hirtella. Asian Journal of Traditional Medicines, 2, 149–153.
22.
ZahoorA, SiddiquiIN, KhanA, AhmadVU, AhmedA, HassanZ, KhanSS, IqbalS. (2010) Two new glycosides from Conyza bonariensis. Natural Product Communications, 5, 1099–1102.
23.
SchumacherB, ScholleS, HölzlJ, KhudeirN, HessS, MüllerCE. (2002) Lignans isolated from valerian: identification and characterization of a new olivil derivative with partial agonistic activity at A1 adenosine receptors. Journal of Natural Products, 65, 1479–1485.
24.
HwangTL, YehSH, LeuYL, ChernCY, HsuHC. (2006) Inhibition of superoxide anion and elastase release in human neutrophils by 3′-isopropoxychalcone via a cAMP-dependent pathway. British Journal of Pharmacology, 148, 78–87.
25.
LuYR, FooLY. (2000) Antioxidant and radical scavenging activities of polyphenols from apple pomace. Food Chemistry, 68, 81–85.
26.
MosmannT. (1983) Rapid colorimetric assay for cellular growth and survival: application to proliferation and cytotoxicity assays. Journal of Immunological Methods, 65, 55–63.
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