The phytochemical investigation of the methanolic extract of the white rot fungus Meripilus giganteus resulted in the isolation and identification of compl mixtures of free fatty acids (1), monoacylglycerols (2), cerebrosides (3), ergosterol (4) and ergosterol peroxide (5). The structures of the isolated lip metabolites (1-5) were determined by chemical and spectroscopic methods. The antioxidant activity of the whole MeOH extract of the fungus was evaluat through in vitro model systems, such as 2,2-diphenyl-1-picrylhydrazyl (DPPH) and superoxide anion. In all two systems, the results indicated that the extra of the fungus showed the same free-radical-scavenging activity with SC50 data of 47.70 μg/mL, compared with the positive control quercetin (DPPH assa None of the isolated compounds (1-5) showed a significant activity. Compounds 2-4 were isolated from Meripilus giganteus for the first time.
LindequistU., NiedermeyerT.H.J., JulichW.D. (2005) The pharmacological potential of mushrooms. eCAM, 2(3), 285–299.
2.
KaramanM., KaišarevićS., SomborskiJ., KebertM., MatavuljM. (2009) Biological activities of the lignicolous fungus Meripilus giganteus (Pers.: Pers.) Karst. Archives of Biological Science, Belgrade, 61, 853–861.
3.
NarbeG., LindequistU., TeuscherE., FrankeP., VainiotaloP., BasnerR. (1991) The chemistry of immunosoppressive active fractions of Meripilus giganteus (Pers. Ex Fr.) Karst., the fiant spore. Die Pharmazie, 46, 738–740.
4.
MizunoM., MinatoK.I., KawakamiS., TatsuokaS., DenpoY., TsuchidaH. (2001) Contents of anti-tumor polysaccharides in certain mushrooms and their immunomodulating activities. Food Science and Technology Research, 7, 31–34.
5.
KafodL.V., KaupinnenM.S., AndersenL.N., ClausenI.G. (1997) An enzyme with galactanase activity and its cDNA sequence from Meripilus giganteus, PCT Int. Appl. WO9732013 A1
6.
SchmidtG., KringsU., Nimtz BergerR.G. (2012) A surfactant tolerant laccase of Meripilus giganteus, World Journal of Microbiology & Biotechnology, 28, 1623–1632.
7.
KalyoncuF., OskayM., SağlamH., ErdoğanT.F., TamerA.U. (2010) Antimicrobial and antioxidant activities of mycelia of 10 wild mushroom species, Journal of Medical Food, 13, 415–419.
8.
TomasiS., Lohézic-Le DévéhatF., SauleauP., BézivinC., BoustieJ. (2004) Cytotoxic activity of methanol extracts from Basidiomycete mushrooms on murine cancer cell lines, Die Pharmazie - An International Journal of Pharmaceutical Sciences, 59, 290–293.
9.
KaramanM., JovinE., MalbasaR., MatavulyM., PopovićM. (2010) Medicinal and edible lignicolous fungi as natural sources of antioxidative and antibacterial agents, Phytotherapy Research, 24, 1473–81
10.
HelrichK. (1990) Association of Official Analytical Chemists, Official Methods of Analysis. 15th Ed. Arlington, VA.
11.
WalkerT.E., LondonR.E., WhaleyT.W., BarkerR., MatwiyoffN.A. (1976) Carbon-13 nuclear magnetic resonance spectroscopy of [1-13C] enriched monosaccharides. signal assignments and orientational dependence of geminal and vicinal carbon-carbon and carbon-hydrogen spin-spin coupling constants, Journal of the American Chemical Society, 98, 5807–5813.
12.
HiguchiR., InagakiM., TogawaK., Miyamoto, KomoriT. (1994) Isolation and structure of cerebrosides of the sea cucumberPentacta australis, Liebigs Annalen der Chemie, 653–658.
13.
JinW., RinehartK.L., Jares-ErijmanE.A. (1994) Ophidiacerebrosides: cytotoxic glycosphingolipids containing a novel sphingosine from a sea star, Journal of Organic Chemistry, 59, 144–147.
14.
ScribeP., GuezennectJ., DaganutJ., PepeC., SaliotA. (1988) Identification of the position and the stereochemistry of the double bond in monounsaturated fatty acid methyl esters by gas chromatography/mass spectrometry of dimethyl disulfide derivatives. Analytical Chemistry, 60, 928–931.
15.
ChengS.Y., WenZ.H., ChiouS.F., TsaiC.W., WangS.K., HsuC.H., DaiC.F., ChiangM.Y., WangW.H., DuhC.Y. (2009) Ceramide and cerebrosides from the octocoral Sarcophyton ehrenbergi.Journal of Natural Products, 72, 465–468.
16.
TanJ.W., DongZ.J., LiuJ.K. (2003) New cerebrosides from the Basidiomycete Cortinarius tenuipes.Lipids, 38, 81–84.
17.
MengT.X., IshikawaH., ShimizuK., OhgaS., KondoR. (2012) A glucosylceramide with antimicrobial activity from the edible mushroom Pleurotus citrinopileatus.Journal of Wood Science, 58, 81–86.
18.
KawaiG., IkedaY. (1985) Structure of biologically active and inactive cerebrosides prepared from Schizophyllum commune, Journal of Lipid Research, 26, 338–343
19.
WrightJ.L.C. (1979) The occurrence of ergosterol and (22E,24R)–24-ethylcholesta-5,7,22-trien-3β-ol in the unicellular chlorophyte Dunaliella tertiolecta.Canadian Journal of Chemistry, 57, 2569–2571.
20.
McCorkindaleN.J. (1976) In The Fungi Vol. 2 Edited by SmithJ.E., and BerryD.R.. Edward Arnold Publishers, London p. 369.
KimD.S., BackN.I., OhS.R., JungK.Y., LeeI.S., KimJ.H., LeeH.K. (1997) anticomplementary activity of ergosterol peroxide from Naematoloma fasciculare and reassignment of NMR data. Archives of Pharmacal Research, 20, 201–205.
23.
GarcíaA., Bocanegra-GarcíaV., Palma-NicolásJ.P., RiveraG. (2012) Recent advances in antitubercular natural products. European Journal of Medicinal Chemistry, 49, 1–23.
24.
BloisM.S. (1958) Antioxidant determination by the use of a stable free radical. Nature, 29, 1199–1200.
25.
HuangD., OuB., PriorR.L. (2005) The chemistry behind antioxidant capacity assays. Journal of Agricultural and Food Chemistry, 53, 1841–1856.
26.
FrankelE.N., MeyerA.S. (2000) The problems of using one dimensional methods to evaluate multifunctional food and biological antioxidants. Journal of the Science of Food and Agriculture, 80, 1925–1941.
