The essential oils from different parts of Litsea cubeba, collected from the highlands of Sarawak, were isolated and their chemical compositions analyzed. This study demonstrated significant variations in the chemical compositions and the chemical profiles of the volatiles and could provide valuable supplementary information on the geographical variations of the species. The fruit essential oil was dominated by citronellal, d-limonene and citronellol, while the leaf oil was high in eucalyptol and α-terpineol. High concentrations of citronellal and citronellol in both the root and bark oils were identified. In the stem, the oil was dominated by eucalyptol, d-limonene and α-terpineol. The activity of the oils against brine shrimp larvae, bacteria, yeast and fungi was determined. The oils were toxic against brine shrimp larvae with LC50 values ranging from 25.1 – 30.9 μL/mL. The oils also demonstrated a wide spectrum of inhibition against microorganisms with inhibition zones between 19.5 – 46.7 mm against Gram-positive bacteria and 10.5 – 90.0 mm against yeast and fungi. However, the oils were not active against Gram-negative bacteria.
KaiY, ChengFW, ChunXY, ZhuFG, RuiQS, ShanSG, ShuSD. (2014) Bioactivity of essential oil of Litsea cubeba from China and its main compounds against two stored product insects. Journal of Asia-Pacific Entomology, 17, 459–466.
2.
KoK, JuntarajumnongW, ChandrapatyaA. (2009) Repellency, fumigant and contact toxicities of Litsea cubeba (Lour.) Persoon against Sitophilus zeamais Motschulsky and Tribolium castaneum (Herbst). Kasetsart Journal (Natural Science), 43, 56–63.
3.
WiartC. (2006) Medicinal Plants of Asia and the Pacific. Taylor & Francis Group LLC, New York. pp 18–21.
4.
SyalizaAH, RuthWWT, JovitaA, TuCL (2009) Small scale hydrodistillation of essential oil from Litsea cubeba. 4th Global Summit on medicinal and Aromatic Plants, 1–5 December 2009. Kuching. p 325.
5.
SyalizaAH, LaiLS, TuCL, JovitaA. (2007) Tenum: An essential oil from the Highlands of Sarawak. Poster session presented at: Proceedings of the International Conference on Natural Resources and Environmental Management and Environmental Safety and Health, (NREM and ESH 2007), 27–29 November 2007, Kuching, Sarawak.
6.
ChaudhurySN. (1981) Muga Silk Industry. Directorate of sericulture and weaving. Government of Assam, Guwahati, India.
ChenY, WangY, ZhouG, LiP, ZhangS. (2008) Key mediators modulating TAG synthesis and accumulation in woody oil plants. Journal of Chinese Medicine, 7, 4743–4749.
9.
HoCL, Jie-PingeO, LiuYC, HungCP, TsaiMC, LiaoPC, WangEI, ChenYL, SuYC. (2010) Compositions and in vitro anticancer activities of the leaf and fruit oils of Litsea cubeba from Taiwan. Natural Product Communications, 5, 617–620.
10.
SiraprapaT, AkeW. (2007) 28-Day repeated dose oral toxicity study of Litsea cubeba essential oil in Sprague-Dawley Rats. Thai Journal of Pharmaceutical Sciences, 31, 74–82.
11.
ZhaoO, ZhouJW, BanDM. (2010) Analysis of volatile oil from different parts of Litsea cubeba (in Chinese). Journal of Chinese Medicinal Material, 33, 1417–1419.
12.
SiLL, ChenYC, HanXJ, ZhanZY, TianSP, CuiQQ, WangYD. (2012) Chemical composition of essential oils of Litsea cubeba harvested from its distribution areas in China. Molecules, 17, 7057–7066.
13.
SaikiaAK, ChetiaD, D'ArrigoM, SmeriglioA, StranoT, RubertoG. (2013) Screening of fruit and leaf essential oils of Litsea cubeba Pers. from north-east India – chemical composition and antimicrobial activity. Journal of Essential Oil Research, 25, 330–338.
14.
NathS, HazarikaA, BaruahA, SarmaK. (1996) Essentials oils of Litsea cubeba (Pers) - An additional chemotype of potential industrial value from Northeastern India. Journal of Essential Oil Research, 8, 575–576.
15.
BighelliA, MuselliA, CasanovaJ, NguyenTT, VuVA, BessièreJM. (2005) Chemical variability of Litsea cubeba leaf oil from Vietnam. Journal of Essential Oil Research, 17, 86–88.
16.
ChengMC, ChengYS. (1983) Composition of the essential oils of Litsea kostermanin Chang, Litsea gerciae Vidal and Litsea cubeba (Lour.) Persoon. Journal of the Chinese Chemical Society, 30, 59–62,
17.
RusliS, NurdjannahN. (1993) The characteristic of some prospective essential oils in Indonesia. Plant Physiology and Biochemistry, 6, 31–37.
18.
LawrenceBM. (1996) Progress in essential oils. Perfumer and Flavorist, 21, 62–68.
19.
OyenLP, NguyenXD. (Eds). (1999) Plant Resources of South-East Asia: Essential oil plants, Backhuys Publishers, Leiden, Netherlands, p 277.
20.
MuchtaridiM, AnasS, AntonA, SlametB. (2005) Analisis senyawa aktif dari minyak atsiri kulit batang Ki Lemo (Litsea cubeba Lour. Pers) yang menekan aktivitas lokomotor mencit. Majalah Farmasi Indonesia, 16, 1–16.
21.
WangHW, LiuYQ. (2010) Chemical composition and antibacterial activity of essential oils from different parts of Litsea cubeba. Chemistry & Biodiversity, 7, 229–235.
22.
WangY, JiangZT, LiR. (2012) Antioxidant activity, free radical scavenging potential and chemical composition of Litsea cubeba essential oil. Journal of Essential Oil Bearing Plants, 15, 134–143.
23.
HuangKC. (1993) The Pharmacology of Chinese Herbs, CRC Press, Boca Raton, Florida, USA, p 234.
24.
HuangXW, FengYC, HuangY, LiHL. (2013) Potential cosmetic application of essential oil extracted from Litsea cubeba fruits from China. Journal of Essential Oil Research, 25, 112–119.
25.
ParkTG, BolchCJ, HallegraeffGM. (2007) Larval Crassostrea bivalve and Artemia brine shrimp bioassays to assess toxicity and micropredation by the heterotrophic dinoflagellates Cryptoperidiniopsis brodyi and Pfiesteria piscicida from Australian waters. Journal of Plankton Research, 29, 791–801.
26.
JiangZ, AkhtarY, BradburyR, ZhangX, IsmanM. (2009) Comparative toxicity of essential oils of Litsea pungens and Litsea cubeba and blends of their major constituents against the Cabbage Looper, Trichoplusia ni. Journal of Agriculture Food Chemistry, 57, 4833–4837.
27.
SeoS, KimJ, LeeS, ShinC, ShinS, ParkI. (2009) Fumigant antitermitic activity of plant essential oils and components from Trachyspermum ammi, Pimenta dioica, Carum carvi, Anethum graveolens, Pelargonium graveolens and Litsea cubeba oils against Japanese termite. Journal of Agricultural and Food Chemistry, 57, 6596–6602.
28.
ChoudhuryS, AhmedR, BarthelA, LeclerP. (1998) Composition of the stem, flowers and fruit oils of Litsea cubeba Pers. from two locations of Assam, India. Journal of Essential Oil Research, 10, 381–386.
29.
StewartD. (2005) Chemistry of essential oils made simple: God's love manifest in molecules, NAPSAC Reproductions, Missouri, pp 235–237
30.
Lis-BalchinM. (2006) Aromatherapy Science: A Guide for Healthcare Professionals. Pharmaceutical Press, London, UK, pp 34–35.
31.
ArceT, ArnH. (2004) Flavornet and human odor space. Online Article. Retrieved July 2013, from Flavornet: http://falvornet.org.index.html
32.
InouyeS, TakizawaT, YamaguchiH. (2001) Antibacterial activity of essential oils and their major constituents against respiratory tract pathogens by gaseous contact. Journal of Antimicrobial Chemotherapy, 47, 565–573.
33.
NikaidoH. (1994) Prevention of drug access to bacterial targets: Permeability barriers and active efflux. Science, 264, 382–388.
34.
BakkaliF, AverbeckS, AverbeckD. (2008) Biological effects of essential oils – A review. Food Chemistry Toxicology, 46, 446–475.
35.
KnoblochK, PauliA, IberB, WeigandH, WeisN. (1989) Antibacterial and antifungal properties of essential oil components. Journal of Essential Oil Research, 1, 118–119.
36.
McLaughlinJ. (1991) Grown gall tumors on potato disc and brine shrimp lethality: Two simple bioassays for higher plants screening and fractionation. In Assay for Bioactivity. HostettmannK. (Ed), Academic Press, San Diego. 2–32.
37.
PrabuseenivasanS, JayakumarM, IgnacimuthuS. (2006) In vitro antibacterial activity of some plant essential oils. BMC Complementary and Alternative Medicine, 6, 1–8.
38.
NCCLS. (2002) Performance Standards for Antimicrobial Susceptibility Testing. 8th Informational Supplement. M100 S12. National Committee for Clinical Laboratory Standards, Villanova.
