This work aimed to assess the effect of combining two pomegranate (Punica granatum L.) cultivars at different rates on the ellagitannin content, antioxidant capacity, and total phenolic content of varietal pomegranate juices. Widely distinct juices made from Mollar de Elche and Wonderful cultivars were used for the elaboration of blended juices. They were stored for 70 days at both room and refrigeration temperatures. This study revealed a significant cultivar effect on the stability of main pomegranate ellagitannins (punicalagins, punicalins, punicalagin-like compound, and ellagic acid derivatives) and on the antioxidant capacity measured by the ABTS+ and DPPH•in vitro assays. Blended juices enhanced and/or retained the initial ellagitannin content and antioxidant capacity of pure juices during storage. Thus, blending varietal juices could be suggested as a promising alternative to the development of fresh juices with a high, stable phytochemical load.
MenaP, Gironés-VilaplanaA, MorenoDA, García-VigueraC. (2011) Pomegranate fruit for health promotion: Myths and realities. Functional Plant Science and Biotechnology, 5, 33–42.
2.
MenaP, MartíN, García-VigueraC. (2014) Chapter 18 - The impact of processing and storage on the (poly)phenolic fraction of pomegranate (Punica granatum L.) juices. In Processing and Impact on Antioxidants in Beverages. PreedyV. (Ed) Academic Press, San Diego. 173–184.
3.
Vázquez-AraújoL, Nuncio-JáureguiPN, CherdchuP, HernándezF, ChambersE, Carbonell-BarrachinaAA. (2014) Physicochemical and descriptive sensory characterization of Spanish pomegranates: Aptitudes for processing and fresh consumption. International Journal of Food Science and Technology, 49, 1663–1672.
4.
MenaP, García-VigueraC, Navarro-RicoJ, MorenoDA, BartualJ, SauraD, MartíN. (2011) Phytochemical characterisation for industrial use of pomegranate (Punica granatum L.) cultivars grown in Spain. Journal of the Science of Food and Agriculture, 91, 1893–1906.
5.
MenaP, MartíN, García-VigueraC. (2014) Varietal blends as a way of optimizing and preserving the anthocyanin content of pomegranate (Punica granatum L.) juices. Journal of Agricultural and Food Chemistry, 62, 6936–6943.
6.
GilMI, Tomás-BarberánFA, Hess-PierceB, HolcroftDM, KaderAA. (2000) Antioxidant activity of pomegranate juice and its relationship with phenolic composition and processing. Journal of Agricultural and Food Chemistry, 48, 4581–4589;
7.
FischerUA, CarleR, KammererDR. (2011) Identification and quantification of phenolic compounds from pomegranate (Punica granatum L.) peel, mesocarp, aril and differently produced juices by HPLC-DAD-ESI/MSn. Food Chemistry, 127, 807–821.
8.
LarrosaM, García-ConesaMT, EspínJC, Tomás-BarberánFA. (2010) Ellagitannins, ellagic acid and vascular health. Molecular Aspects of Medicine, 31, 513–539;
9.
Rodriguez-MateosA, VauzourD, KruegerCG, ShanmuganayagamD, ReedJ, CalaniL, MenaP, DelRio D, CrozierA. (2014) Bioavailability, bioactivity and impact on health of dietary flavonoids and related compounds: an update. Archives of Toxicology, 88, 1803–1853;
10.
ZanottiI, Dall'AstaM, MenaP, MeleL, BruniR, RayS, Del RioD. (2015) Atheroprotective effects of (poly)phenols: focus on cell cholesterol metabolism. Food & Function, 6, 13–31.
11.
AlighourchiH, BarzegarM, AbbasiS. (2008) Anthocyanins characterization of 15 Iranian pomegranate (Punica granatum L.) varieties and their variation after cold storage and pasteurization. European Food Research and Technology, 227, 881–887;
12.
MiguelG, FontesC, MartinsD, NevesA, AntunesD. (2007) Evolution of anthocyanins in pomegranate juice (Punica granatum L.) of two cultivars “Mollar” and “Assaria” during cold storage. Sciences des Aliments, 27, 431–438.
13.
Hernández-HerreroJA, FrutosMJ. (2011) Degradation kinetics of pigment, colour and stability of the antioxidant capacity in juice model systems from six anthocyanin sources. International Journal of Food Science and Technology, 46, 2550–2557;
14.
SadilovaE, StintzingFC, KammererDR, CarleR. (2009) Matrix dependent impact of sugar and ascorbic acid addition on color and anthocyanin stability of black carrot, elderberry and strawberry single strength and from concentrate juices upon thermal treatment. Food Research International, 42, 1023–1033.
15.
BorgesG, CrozierA. (2012) HPLC-PDA-MS fingerprinting to assess the authenticity of pomegranate beverages. Food Chemistry, 135, 1863–1867;
16.
CalaniL, BeghèD, MenaP, Del RioD, BruniR, FabbriA, Dall'AstaC, GalavernaG. (2013) Ultra-HPLC-MSn (poly)phenolic profiling and chemometric analysis of juices from ancient Punica granatum L. cultivars: A nontargeted approach. Journal of Agricultural and Food Chemistry, 61, 5600–5609;
17.
MenaP, CalaniL, Dall'AstaC, GalavernaG, García-VigueraC, BruniR, CrozierA, Del RioD. (2012) Rapid and comprehensive evaluation of (poly)phenolic compounds in pomegranate (Punica granatum L.) juice by UHPLC-MSn. Molecules, 17, 14821–14840.
18.
BorgesG, MullenW, CrozierA. (2010) Comparison of the polyphenolic composition and antioxidant activity of European commercial fruit juices. Food & Function, 1, 73–83.
19.
Aguilera-CarboA, AugurC, Prado-BarraganLA, Favela-TorresE, AguilarCN. (2008) Microbial production of ellagic acid and biodegradation of ellagitannins. Applied Microbiology and Biotechnology, 78, 189–199.
20.
González-MolinaE, MorenoDA, García-VigueraC. (2009) A new drink rich in healthy bioactives combining lemon and pomegranate juices. Food Chemistry, 115, 1364–1372;
21.
Pérez-VicenteA, SerranoP, AbellánP, García-VigueraC. (2004) Influence of packaging material on pomegranate juice colour and bioactive compounds during storage. Journal of the Science of Food and Agriculture, 84, 639–644.
22.
ZafrillaP, FerreresF, Tomás-BarberánFA. (2001) Effect of processing and storage on the antioxidant ellagic acid derivatives and flavonoids of red raspberry (Rubus idaeus) jams. Journal of Agricultural and Food Chemistry, 49, 3651–3655.
23.
TalcottST, LeeJH. (2002) Ellagic acid and flavonoid antioxidant content of muscadine wine and juice. Journal of Agricultural and Food Chemistry, 50, 3186–3192.
24.
FischerUA, DettmannJS, CarleR, KammererDR. (2011) Impact of processing and storage on the phenolic profiles and contents of pomegranate (Punica granatum L.) juices. European Food Research and Technology, 233, 797–816.
25.
HagerTJ, HowardLR, PriorRL. (2010) Processing and storage effects on the ellagitannin composition of processed blackberry products. Journal of Agricultural and Food Chemistry, 58, 11749–11754.
26.
De VasconcelosMC, BennettR, RosaE, Ferreira-CardosoJV, NunesF. (2010) Industrial processing of chestnut fruits (Castanea sativa Mill.) -Effects on nutrients and phytochemicals Acta Horticulturae, 866, 611–617.
27.
MenaP, MartíN, SauraD, ValeroM, García-VigueraC. (2013) Combinatory effect of thermal treatment and blending on the quality of pomegranate juices. Food and Bioprocess Technology, 6, 3186–3199.
28.
TzulkerR, GlazerI, Bar-IlanI, HollandD, AviramM, AmirR. (2007) Antioxidant activity, polyphenol content, and related compounds in different fruit juices and homogenates prepared from 29 different pomegranate accessions. Journal of Agricultural and Food Chemistry, 55, 9559–9570.
29.
GancelAL, FeneuilA, AcostaO, PérezAM, VaillantF. (2011) Impact of industrial processing and storage on major polyphenols and the antioxidant capacity of tropical highland blackberry (Rubus adenotrichus). Food Research International, 44, 2243–2251.
