Free accessResearch articleFirst published online 2014-12
A GC-FID Validated Method for the Quality Control of Eucalyptus globulus Raw Material and its Pharmaceutical Products,and GC-MS Fingerprinting of 12 Eucalyptus Species
In this work we have validated a method to standardize and control the quality of Eucalyptus globulus raw material and phytomedicines containing either the essential oil or the fluid extract of this plant in the final formulation. Internal standardization provided a simple, fast, and reproducible GC-FID analytical method that accurately quantified 1,8-cineol in different E. globulus sub-products, such as its essential oil, dried leaves, fluid extract, and syrup. In addition, GC-MS identification of the main compounds of E. globulus species afforded fingerprints for the qualitative analysis of different Eucalyptus species.
CalixtoJ.B. (2000) Efficacy, safety, quality control, marketing and regulatory guidelines for herbal medicines (phytotherapeutic agents). Brazilian Journal of Medical and Biological Research, 33, 179–189.
2.
BarnesJ. (2003) Quality, efficacy and safety of complementary medicines: fashions, facts and the future. Part I. Regulation and quality. British Journal of Clinical Pharmacology, 55, 226–233.
3.
SousaJ.P.B., BuenoP.C.P., GregórioL.E., Da Silva FilhoA.A., FurtadoNajc, SousaM.L., BastosJ.K. (2007) A reliable quantitative method for the analyses of phenolic compounds in Brazilian propolis by reverse high performance liquid chromatography. Journal of Separation Science, 30, 2656–2665.
4.
GongF., LiangY.Z., XieP.S., ChauF.T. (2003) Information theory applied to chromatographic fingerprint of herbal medicine for quality control. Journal of Chromatography A, 1002, 25–40.
5.
AlonsoJ. (2004) Tratado de Fitofármacos y Nutracéuticos (1st ed.), Corpus libros, Rosário.
6.
CimangaK., KambuK., TonaL., ApersS., De BruyneT., HermansN., TotteJ., PietersL., VlietinckA.J. (2002) Correlation between chemical composition and antibacterial activity of essential oils of some aromatic medicinal plants growing in the Democratic Republic of Congo. Journal of Ethnopharmacology, 79, 212–220.
7.
Daroui-MokaddemH., KaboucheA., BouachaM., SoumatiB., El-AzzounyA., BruneauC, KaboucheZ. (2010) GC/MS analysis and antimicrobial activity of the essential oil of fresh leaves of Eucalyptus globulus, and leaves and stems of Smyrnium olusatrum from Constantine (Algeria). Natural Product Communications, 5, 1669–1672.
8.
TroncosoC., PerezC., HernandezV., Sanchez-OlateM., RiosD., MartinA.S., BecerraJ. (2013) Induction of defensive response in Eucalyptus globulus plants and its persistence in vegetative propagation. Natural Product Communications, 8, 397–400.
9.
BlumenthalM. (1998) The Complete Commission E Monographs.Integration Medicine Communications, Massachusetts.
10.
GruenwaldJ., BrenlerT., JaenickeC. (2000) PDR for Herbal Medicines.Medical Economics Company Inc, Montvale.
11.
Brasil, Ministério da Saúde, Agência Nacional de Vigilância Sanitária (ANVISA). (2004) Resolução RE n. 89 de 16 de março de 2004 - Lista para registro simplificado de fitoterápicos.
12.
World Health Organization (WHO). (1992) Quality control methods for medicinal plant material.World Health Organization, Geneva.
13.
Farmacopéia Brasileira 4.ed. Parte I. (1996) Eucalipto (Monografia 27). Ateneu, São Paulo.
14.
YoudenW.J., SteinerE.H. (1975) Statistical manual of AOAC – Association of Official Analytical Chemistry.AOAC, Washington.
15.
ClementR.E. (1990) Gas Chromatography: Biochemical, Biomedical and Clinical Applications.John Wiley and Sons Inc., New York.
16.
Farmacopéia Brasileira 1.ed. Parte I. (1926) Companhia Editora Nacional, São Paulo.
