Chemical Composition of Angelica pancicii Essential Oil Determined by Liquid and Headspace GC-MS Techniques

Abstract

The essential oil of the Balkan endemic species, Angelica pancicii, obtained by hydrodistillation, was analyzed by GC and GC-MS, applying the liquid injection mode. These results were compared with the chemical composition of volatiles achieved by the “headspace” injection mode, followed by GC and GC-MS (HS-GC-MS). A total of 40 compounds were identified in the essential oil (98.8% of the total oil) and 44 by HS-GC-MS (99.8% of the total oil). The most abundant class of compounds in both cases was monoterpenoids, which formed 92.7% of the essential oil (97.7% by HS-GC-MS) of total identified compounds. The major components in both cases were β-phellandrene (54.9% and 60.1%, respectively), α-pinene (14.5% and 20.1%, respectively) and α-phellandrene (4.5% and 4.3%, respectively).

Keywords

Angelica pancicii Essential oil Chemical composition GC-MS Headspace

References

Shchipitsyna

O.S.

, Efremov

A.A.

(2011) Composition of Ethereal Oil Isolated from Various Vegetative Parts of Angelica from the Siberian Region, Russian Journal of Bioorganic Chemistry, 37, 888–892.

Andreev

(1984) Angelica pancicii Vand., A. archangellica L. In Red Data Book of the People's Republic of Bulgaria. Vol. 1 Plants. Velčev

(Ed). Publishing House of the Bulgarian Academy of Sciences, Sofia, Bulgaria. 266.

Peev

, Anchev

, Ivanova

, Kozhucharov

, Petrova

, Tzoneva

(1993) Biodiversity of the higher plants in Bulgaria. In National Strategy for Preservation of Biodiversity. Vol. 1, Sakalyan

, Meini

(Eds), Biodiversity Support Program (in Bulgarian), 73–124.

Euro Med Plant Base Website, source: Hand, R. (2011) http://www.emplantbase.org/

(a) Peev

(1982) Angelica L. In Flora Reipublicae Popularis Bulgaricae. Vol. 8, Velčev V (ed), In Aedibus Acad. Sci. Bulgaricae, Serdicae (in Bulgarian). 207–211; (b) Dzhuneva S, Konakchiev A., Evstatieva L. (2008) Preliminary comparative study of the essential oils of the species of genus Angelica L. growing in Bulgaria. Proceedings of the Fifth Conference on Medicinal and Aromatic Plants of Southeast European Countries, (5th CMAPSEEC), Brno, Czech Republic, 140; (c) Yankova E. (2004) Comparative anatomical study of the fruits of Angelica pancicii and Angelica sylvestris (Apiaceae) distributed in the Bulgarian flora. Phytologia Balcanica, 10, 61–67.

Yankova

, Cherneva

(2007) Notes on the species distribution of genus Angelica in Bulgaria. Phytologia Balcanica, 13, 189–192.

(a) Bernard

(2001) Essential Oils of Three Angelica L. Species Growing in France. Part II: Fruit Oils. Journal of Essential Oil Research, 13, 260–263; (b) Sigurdsson S, Ögmundsdottir HM, Gudbjarnason S. (2005) The Cytotoxic Effect of Two Chemotypes of Essential Oils from the Fruits of Angelica archangelica L. Anticancer Research, 25, 1877–1880.

(a) Özek

, Özek

, Başer

K.H.C.

, Duran

, Sagiroglu

(2008) Composition of the essential oils of Angelica sylvestris L. var. sylvestris isolated from the fruits by different isolation techniques. Journal of Essential Oil Research, 20, 408–411; (b) Van den Dool H, Kratz PD. (1963) A generalization of the retention index system including linear temperature programmed gas-liquid partition chromatography. Journal of Chromatography A, 11, 463–471; (c) Adams RP. (2007) Identification of Essential Oil Components by Gas Chromatography-Mass Spectroscopy. Allured Publishing Corporation, Carol Stream, Illinois; (d) National Institute of Standards and Technology, NIST Chemistry WebBook, Nist Standard Reference database Website. http://webbook.nist.gov/chemistry/ (2005).

Stein

S.E.

(1990) National Institute of Standards and Technology (NIST) Mass Spectral Database and Software, Version 3.02.