The chemical composition of the essential oils of two endemic plants of Turkey, Stachys rupestris Montbret et Aucher ex Benth. and Salvia heldreichiana Boiss. ex Benth., were obtained by hydrodistillation and studied by GC and GC–MS. In all, 46 compounds were identified, 22 for S. rupestris accounting for 94.6 % of the total oil and 30 for S. heldreichiana, accounting for 91.9 % of the total oil. The presence of diterpenoids (50.7%) characterized the oil from S. rupestris, while S. heldreichiana oil was rich in oxygenated sesquiterpenes (78.9%). The essential oils were evaluated for their in vitro potential cytotoxic activity on three human cancer cell lines. The oil of S. rupestris showed the higher antiproliferative activity against PC-3 and MCF-7 cancer cell lines.
BakkaliF., AverbeckS., AverbeckD., IdaomarM. (2008) Biological effects of essential oils –A review. Food and Chemical Toxicology, 46, 446–475.
2.
De MartinoL., ManciniE., MarandinoA., Rolim de AlmeidaL.F., De FeoV. (2012) Chemistry and antigerminative activity of essential oils and monoterpenoids from Mediterranean plants. Current Bioactive Compounds, 8, 13–49.
3.
AdorjanB., BuchbauerG. (2010) Biological properties of essential oils: an updated review. Flavour and Fragrance Journal, 25, 407–426.
4.
CarsonC.F., HammerK.A. (2011) Chemistry and bioactivity of essential oils. In Lipids and Essential Oils as Antimicrobial Agents, ThormarH. (Ed.). John Wiley and Sons, Chichester, 203–238.
5.
El-MeleigyM.A., AhmedM.E., ArafaR.A., EbrahimN.A., El-KholanyE.E. (2010) Cytotoxicity of four essential oils on some human and bacterial cells. Journal of Medical Case Reports, 5, 143–159.
6.
BostancıogluR.B., KürkçüogluM., BaşerK.H.C., KoparalA.T. (2012) Assesment of anti-angiogenic and anti-tumoral potentials of Origanum onites L. essential oil. Food and Chemical Toxicology, 50, 2002–2008.
7.
ZuY., YuH., LiangL., FuY., EfferthT., LiuX., WuN. (2010) Activities of ten essential oils towards Propionibacterium acnes and PC-3, A-549 and MCF-7 cancer cells. Molecules, 15, 3200–3210.
8.
MosaddikM.A. (2003) In vitro cytotoxicity of tanshinones isolated from Salvia miltiorrhiza Bunge against P388 lymphocytic leukemia cells. Phytomedicine, 10, 682–685.
9.
De SousaA.C., AlvianoS., BlankA.F., AlvesP.B., AlvianoC.S., GattassC.R. (2004) Melissa officinalis L. essential oil: antitumoral and antioxidant activities. Journal of Pharmacy and Pharmacology, 56, 677–681.
10.
BallP.W. (1972) Stachys L. In Flora Europaea, TutinT.G. (Ed.), Cambridge University Press, Cambridge, 154.
11.
SkaltsaH., BermejoP., LazariD., SilvanA.M., SkaltsounisA.L., SanzA., AbadM.J. (2000) Inhibition of progtaglandin E2 and leukotriene C4 in mouse peritoneal macrophages and thromboxane B2 production in human platelets by flavonoids from Stachys chrysantha and Stachys candida. Biological and Pharmaceutical Bulletin, 23, 47–53.
12.
KhanaviM., ManayiA., LotfiM., AbbasiR., MajdzadehM., OstadN. (2012) Investigation of cytotoxic activity in four Stachys species from Iran. Iranian Journal of Pharmaceutical Research, 11, 589–593.
13.
GorenA.C., PiozziF., AkcicekE., KılıçT., CarıkcıS, MozioĝluE., SetzerW.N. (2011) Essential oil composition of twenty-two Stachys species (mountain tea) and their biological activities. Phytochemistry Letters, 4, 448–453.
14.
HartwellJ.L. (1982) Plants used against cancer: a survey.Quarterman Publications Inc., Massachusetts.
15.
AmirghofranZ., BahmaniM., AzadmehrA., JavidniaK. (2006) Anticancer effects of various Iranian native medicinal plants on human tumor cell lines. Neoplasma, 53, 428–433.
16.
OztürkM., DuruM.E., Aydogmus-OztütrkF., HarmandarM., MahliçliM., KolakU., UlubelenA. (2009) GC-MS analysis and antimicrobial activity of essential oil of Stachys cretica subsp. smyrnaea. Natural Product Communications, 4, 109–114.
17.
LazarevićJ.S., PalićR.M., RadulovićN.S., RistićN.R., StojanovićG.S. (2010) Chemical composition and screening of the antimicrobial and antioxidative activity of extracts of Stachys species. Journal of Serbian Chemical Society, 75, 1347–1359.
ŞerbetçiT., DemirciB., Bozkurt-Güzelç, KültürŞ, ErgüvenM., BaşerK.H.C. (2010) Essential oil composition, antimicrobial and cytotoxic activities of two endemic Stachys cretica subspecies (Lamiaceae) from Turkey. Natural Product Communications, 5, 1369–1374.
20.
KhanaviM., ManayiA., LotfiM., AbbasiR., MajdzadehM., OstadS.N. (2012) Investigation of cytotoxic activity in four Stachys species from Iran. Iranian Journal of Pharmaceutical Research, 11, 589–593.
21.
CellatK., GülŞ, EverestA. (2011) Investigation of essential oil composition of Stachys rupestris Montbret et Aucher ex Bentham from Mersin. Poster discussion, Lokman Hekim Journal Supplement, pp. 58.
22.
UlubelenA., BirmanH., ÖksüzS., TopçuG., KolakU., BarlaA., VoelterW. (2002) Cardioactive diterpenes from Salvia eriophora. Planta Medica, 68, 818–821.
23.
WuY.B., NiZ.Y., ShiQ.W., DongM., KiyotaH., GuY.C., CongB. (2012) Constituents from Salvia species and their biological activities, A review. Chemical Reviews, 112, 5967–6026.
24.
BasalmaD., GürbüzB., SarihanE.O., İpekA., ArslanN., DuranA., KendirH. (2007) Essential oil composition of Salvia heldreichiana Boiss. ex Bentham described endemic species from Turkey. Asian Journal of Chemistry, 19, 2130–2134.
25.
AkinM., DemirciB., BagcıY., BaserK.H.C. (2010) Antibacterial activity and composition of the essential oils of two endemic Salvia sp. from Turkey. African Journal of Biotechnology, 9, 2322–2327.
26.
PiozziF., BrunoM. (2011) Diterpenoids from roots and aerial parts of the genus Stachys. Records of Natural Products, 5, 1–11.
27.
KamatouG.P.P., Van ZylR.L., Van VuurenS.F., FigueiredoA.C., BarrosoJ.G., PedroL.G., ViljoenA.M. (2008) Seasonal variation in essential oil composition, oil toxicity and the biological activity of solvent extracts of three South African Salvia species. South African Journal of Botany, 74, 230–237.
28.
CardileV., RussoA., FormisanoC., RiganoD., SenatoreF. (2009) Essential oils of Salvia bracteata and Salvia rubifolia from Lebanon: chemical composition, antimicrobial activity and inhibitory effect on human melanoma cells. Journal of Ethnopharmacology, 126, 265–272.
29.
SertelS., EichhornT., PlinkertP.K., EfferthT. (2011) Anticancer activity of Salvia officinalis essential oil against HNSCC cell line (UMSCC1). HNO, 59, 1203–1208.
30.
ConfortiF., MenichiniF., FormisanoC., RiganoD., SenatoreF., ArnoldN.A., PiozziF. (2009) Comparative chemical composition, free radical-scavenging and cytotoxic properties of essential oils of six Stachys species from different regions of the Mediterranean area. Food Chemistry, 116, 898–905.
31.
JunN.J., MosaddikA., MoonJ.Y., JangK.C., LeeD.S., AhnK.S., ChoS.K. (2011) Cytotoxic activity of (3-caryophyllene oxide isolated from Jeju Guava (Psidium cattleianum Sabine) leaf. Records of Natural Products, 5, 242–246.
32.
European Pharmacopoeia, 5th ed. (2004) Council of Europe, Strasbourg Cedex, France, Volume I, 217–218.
33.
JenningsW., ShibamotoT. (1980) Qualitative Analysis of Flavour and Fragrance Volatiles by Glass Capillary Gas Chromatography.Academic Press, New York, NY, USA.
34.
DaviesN.W. (1990) Gas chromatographic retention indices of monoterpenes and sesquiterpenes on methyl silicone and Carbowax 20M phases. Journal of Chromatography, 503, 1–24.
35.
AdamsR.P. (2007) Identification of Essential Oil Components by Gas Chromatography/Mass Spectrometry, 4th Ed., Carol Stream, Illinois, USA.
36.
MosmannT., (1983) Rapid colorimetric assay for cellular growth and survival: Application to proliferation and cytotoxicity assays. Journal of Immunological Methods, 65, 55–63.
