Broussonetia papyrifera (L.) Vent. and Lonicera japonica Thunb. have been used in recent medicinal research for their antioxidative and anti-inflammatory properties. The present study investigated the therapeutic efficacy of B. papyrifera and L. japonica ethanolic extracts in a murine model of ovalbumin-induced asthma, in which intra-peritoneal (IP) injections and aerosol ovalbumin delivery were used to induce allergic asthma. Bronchioalveolar lavage fluid (BALF), serum samples, lungs and livers were collected from the experimental groups. In the groups treated with B. papyrifera and L. japonica extracts, CD3, CD4, serum IgE and IL-4 levels; activities of matrix metalloproteinase (MMP)-2 and MMP-9; and eotaxin levels in the BALF significantly decreased to near normal levels. Results of a histopathological analysis showed that the level of inflammation and mucous secretions reduced in the treated groups compared to the corresponding levels in the other groups. Moreover, results of a serum enzymatic analysis showed the non-toxic nature of the extracts in the B. papyrifera and L. japonica treated groups. Taken together, these results clearly indicate that the B. papyrifera and L. japonica extracts may be very effective against asthma and inflammation related diseases.
BurrM.L., WatD., EvansC., DunstanF.D., DoullI.J. (2006) Asthma prevalence in 1973, 1988 and 2003. Thorax, 61, 296–299.
2.
Centers for Disease Control and prevention (CDC). (2011) Vital signs: asthma prevalence, disease characteristics, and self-management education: United States, 2001–2009. MMWR. Morbidity and Mortality Weekly Report, 60, 547–552.
3.
RomagnaniS. (2000) The role of lymphocytes in allergic disease. Journal of Allergy and Clinical Immunology, 105, 399–408.
4.
HsiehC.S., HeimbergerA.B., GoldJ.S., O'GarraA., MurphyK.M. (1992) Differential regulation of T helper phenotype development by interleukins 4 and 10 in an alpha beta T-cell-receptor transgenic system. Proceedings of the National Academy of Sciences of the United States of America, 89, 6065–6069.
5.
JutelM., PichlerW.J., SkrbicD., UrwylerA., DahindenC., MullerU.R. (1995) Bee venom immunotherapy results in decrease of IL-4 and IL-5 and increase of IFN-gamma secretion in specific allergen-stimulated T cell cultures. Journal of Immunology, 154, 4187–4194.
6.
EliasJ.A., LeeC.G., ZhengT., MaB., HomerR.J., ZhuZ. (2003) New insights into the pathogenesis of asthma. Journal of Clinical Investigation, 111, 291–297.
7.
LarcheM., RobinsonD.S., KayA.B. (2003) The role of T lymphocytes in the pathogenesis of asthma. Journal of Allergy and Clinical Immunology, 111, 450–463.
8.
HoontrakoonR., KaileyJ.M., BrattonD.L. (1999) IL-4 and TNF alpha synergize to enhance eosinophil survival. Journal of Allergy and Clinical Immunology, 103, S239–S239.
9.
KellyE.A., BusseW.W., JarjourN.N. (2000) Increased matrix metalloproteinase-9 in the airway after allergen challenge. American Journal of Respiratory and Critical Care Medicine, 162, 1157–1161.
10.
GoetzlE.J., BandaM.J., LeppertD. (1996) Matrix metalloproteinases in immunity. Journal of Immunology, 156, 1–4.
11.
SohnH.Y., KwonC.S., SonK.H. (2010) Fungicidal effect of prenylated flavonol, papyriflavonol A, isolated from Broussonetia papyrifera (L.) Vent. against Candida albicans. Journal of Microbiology and Biotechnology, 20, 1397–1402.
12.
KwakW.J., MoonT.C., LinC.X., RhynH.G., JungH., LeeE., KwonD.Y., SonK.H., KimH.P., KangS.S., MurakamiM., KudoI., ChangH.W. (2003) Papyriflavonol A from Broussonetia papyrifera inhibits the passive cutaneous anaphylaxis reaction and has a secretory phospholipase A2-inhibitory activity. Biological and Pharmaceutical Bulletin, 26, 299–302.
13.
RyuK.H., RheeH.I., KimJ.H., YooH., LeeB.Y., UmK.A., KimK., NohJ.Y., LimK.M., ChungJ.H. (2010) Anti-inflammatory and analgesic activities of SKLJI, a highly purified and injectable herbal extract of Lonicera japonica. Bioscience, Biotechnology, and Biochemistry, 74, 2022–2028.
14.
JinJ.H., LimH., KwonS.Y., SonK.H., KimH.P. (2010) Anti-inflammatory activity of the total flavonoid fraction from Broussonetia papyrifera in combination with Lonicera japonica. Biomolecules and Therapeutics, 18, 197–204.
15.
MullerV., GalffyG., EszesN., LosonczyG., BizziA., NicoliniG., ChrystynH., TamasiL. (2011) Asthma control in patients receiving inhaled corticosteroid and long-acting beta2-agonist fixed combinations. A real-life study comparing dry powder inhalers and a pressurized metered dose inhaler extrafine formulation. BMC Pulmonary Medicine, 11, 40.
16.
PelaiaG., GallelliL., RendaT., RomeoP., BuscetiM.T., GrembialeR.D., MaselliR., MarsicoS.A., VatrellaA. (2011) Update on optimal use of omalizumab in management of asthma. Journal of Asthma and Allergy, 4, 49–59.
17.
ChippsB.E., FligliomeniM., SpectorS. (2012) Omalizumab: An update on efficacy and safety in moderate-to-severe allergic asthma. Allergy and Asthma Proceedings, 33, 377–385.
18.
ChungK.F., CaramoriG., AdcockI.M. (2009) Inhaled corticosteroids as combination therapy with beta-adrenergic agonists in airways disease: present and future. European Journal of Clinical Pharmacology, 65, 853–871.
19.
BruniF.M., De LucaG., VenturoliV., BonerA.L. (2009) Intranasal corticosteroids and adrenal suppression. Neuroimmunomodulation, 16, 353–362.
20.
KovacevicA., SchwahnB., SchusterA. (2010) Hyperlactic acidosis as metabolic side-effect of albuterol and theophylline in acute severe asthma. Klinische Pädiatrie, 222, 271–272.
21.
BurggraafJ., WestendorpR.G., in't VeenJ.C., SchoemakerR.C., SterkP.J., CohenA.F., BlauwG.J. (2001) Cardiovascular side effects of inhaled salbutamol in hypoxic asthmatic patients. Thorax, 56, 567–569.
22.
ShinI.S., LeeM.Y., LimH.S., HaH., SeoC.S., KimJC, and ShinH.K. (2012) An extract of Crataegus pinnatifida fruit attenuates airway inflammation by modulation of matrix metalloproteinase-9 in ovalbumin induced asthma. PLos One, 7, e45734.
23.
PanahiY., TavanaS., SahebkarA., MasoudiH., MadanchiN. (2012) Impact of adjunctive therapy with Chlorella vulgaris extract on antioxidant status, pulmonary function, and clinical symptoms of patients with obstructive pulmonary diseases. Scientia Pharmaceutica, 80, 719–730.
24.
DiamantZ., MantzouranisE., BjermerL. (2009) Montelukast in the treatment of asthma and beyond. Expert Review of Clinical Immunology, 5, 639–658.
25.
de SouzeF.C., GobbatoN.B., MacielR.G., PradoC.M., MartinsM.D., LeickE.A., TiberioI.F. (2013) Effects of corticosteroid, montelukast and iNOS inhibition on distal lung with chronic inflammation. Respiratory Physiology and Neurobiology, 185, 435–445.
26.
RohS.S., KimS.H., LeeY.C., SeoY.B. (2008) Effects of radix adenophorae and cyclosporine A on an OVA-induced murine model of asthma by suppressing to T cells activity, eosinophilia, and bronchial hyperresponsiveness. Mediators of Inflammation, 781425.
27.
MajoriM., PiccoliM.L., BertaccoS., CuomoA., CantiniL., PesciA. (1997) Inhaled beclomethasone dipropionate downregulates CD4 and CD8 T-lymphocyte activation in peripheral blood of patients with asthma. Journal of Allergy and Clinical Immunology, 100, 379–382.
28.
CoffmanR.L., OharaJ., BondM.W., CartyJ., ZlotnikA., PaulW.E. (1986) B cell stimulatory factor-1 enhances the IgE response of lipopolysaccharide-activated B cells. Journal of Immunology, 136, 4538–4541.
29.
DoucetC., Brouty-BoyeD., Pottin-ClemenceauC., JasminC., CanonicaG.W., AzzaroneB. (1998) IL-4 and IL-13 specifically increase adhesion molecule and inflammatory cytokine expression in human lung fibroblasts. International Immunology, 10, 1421–1433.
30.
DabbaghK., TakeyamaK., LeeH.M., UekiI.F., LausierJ.A., NadelJ.A. (1999) IL-4 induces mucin gene expression and goblet cell metaplasia in vitro and in vivo. Journal of Immunology, 162, 6233–6237.
TanR.J., FattmanC.L., NiehouseL.M., TobolewskiJ.M., HanfordL.E., LiQ., MonzonF.A., ParksW.C., OuryT.D. (2006) Matrix metalloproteinases promote inflammation and fibrosis in asbestos-induced lung injury in mice. American Journal of Respiratory Cell and Molecular Biology, 35, 289–297.
33.
CataldoD., MunautC., NoelA., FrankenneF., BartschP., FoidartJ.M., LouisR. (2000) MMP-2- and MMP-9-linked gelatinolytic activity in the sputum from patients with asthma and chronic obstructive pulmonary disease. International Archives of Allergy and Immunology, 123, 259–267.
34.
LeeM.Y., LeeN.H., JungD., LeeJ.A., SeoC.S., LeeH., KimJ.H., ShinH.K. (2010) Protective effects of allantoin against ovalbumin (OVA)-induced lung inflammation in a murine model of asthma. International Immunopharmacology, 10, 474–480.
