Glucosinolates (GLs) were characterized in the seed and root of Aurinia leucadea (Guss.) C. Koch and A. sinuata (L.) Griseb., and quantified according to the ISO 9167–1 official method based on the HPLC analysis of desulfo-GLs. Glucoalyssin (GAL, 1), glucobrassicanapin (GBN, 2) and glucoberteroin (GBE, 3) were the major GLs identified in A. leucadea and A. sinuata. GC/MS analysis of the volatile fractions obtained after enzyme hydrolysis showed that they mostly contain isothiocyanates (ITCs) originating from the parent GLs. On this basis and from previous reports, C-5 alkyl GLs 1, 2, and 3 can be considered as chemotaxonomic markers of the Aurinia genus.
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