Russian propolis from the Perm Region was studied by GC-MS, its antibacterial activity was determined, and the bioactive compounds (total phenolics and flavonoids) were quantified by spectrophotometric methods. It was found that Permian propolis belonged to a bee-glue type of triple plant origin: aspen-birch-poplar. Such propolis type has not yet been described. It contains many constituents which could be responsible for diverse biological activities: antibacterial, antifungal, antiviral, antioxidant, anti-inflammatory, and anti-tumor. The combination of the protective excretions of three plant species, resulting in the presence of secondary plant metabolites of many different structural classes gives reason to believe that the pharmacological actions are implemented in different, complementary mechanisms. Together with the detected antibacterial activity, this observation leads to the expectation that such combination could be especially effective in treatment of infections of different origin..
PopravkoAS, SokolovIV, TorgovIV. (1984) [Derivatives of unsaturated aromatic alcohols in propolis and styrax resin]. Khimiya prirodnykh soedinenii, 19, 152-160 (in Russian).
5.
SokolovIV, TorgovIV. (1989) [Conjugates of trans-coniferyl alcohol in propolis and its sources]. Khimiya prirodnykh soedinenii, 24, 319-324 (in Russian).
6.
PopravkoSA. (1977) [Chemical composition of propolis and its standardization]Pchelovodstvo, 97, 21-23 (in Russian).
7.
PopravkoSA. (1978) Chemical composition of propolis, its origin and standardization. In A remarkable hive product: propolis.HarnajV. (Ed.) Apimondia Publishing House, Bucharest. 15–18.
8.
LiF, AwaleS, TezukaY, KadotaS (2010) Cytotoxicity of constituents from Mexican propolis against a panel of six different cancer cell lines. Natural Product Communications, 5, 1601–1606.
9.
VeraN, SolórzanoE, OrdoñezR, MaldonadoL, BedascarrasbureE, IslaMI. (2011) Chemical composition of Argentinean propolis collected in extreme regions and its relation with antimicrobial and antioxidant activities. Natural Product Communications, 6, 823–827.
10.
FidalgoLM, RamosIS, ParraMG, Cuesta-RubioO, HernándezIM, FernándezMC, PiccinelliAL, RastrelliL. (2011) Activity of Cuban propolis extracts on Leishmania amazonensis and Trichomonas vaginalis. Natural Product Communications, 6, 973–976.
PopravkoSA, TikhomirovaVI, VulfsonNS. (1985). [Comparative study of the chemical composition and biological activity of propolis and its sources]. In Propolis, Apimondia Publishing House, Bucharest. 35-37 (in Russian).
13.
PletnevMY, SolovevaTS, BlinovDA. (2011) [Colloid-chemical properties of water dispersion of propolis obtained by change of solvent]. Vestnik MITKhT, 6, 82-84 (in Russian).
14.
ZagretdinovAF. (2009) [Influence of propolis collection on the dynamics of brood rearing and productivity of bee colonies]. Agrarnyi vestnik Urala, 60, 37–39 (in Russian).
15.
BanjkovskiiVV, PopovDM, LyakinaMN, BanjkovskiiDV. (2009). [Purificarion of raw propolis]. Farmatziya, 6, 37–39 (in Russian).
16.
SarafAS, KalmykovAA, DenisovAK. (2007) [On the effect of the application of a new original domestic preparation Prostopin in treatment of acute and chronic prostatitis and benign hyperplasia of the prostate gland]. Trudnii pacient, 12-13, 13–15 (in Russian).
17.
EvdokimovVV. (2010) [Treatment of acute and chronic prostatitis]. Trudnii pacient, 73, 40-44 (in Russian).
18.
FaramazyanA. (2007) [Propolis in treatment of stomach and duodenal ulcers]. Pchelovodstvo, 56, 56–58 (in Russian).
19.
LazebnikLB, DubtsovaEA, KsjianenkoVI, KomissarenkoIA. (2007) [Propolis, its antimicrobial properties and use in treatment of gastritis and ulcers]. Traditsionnaya meditsina, 11, 46–50 (in Russian).
20.
BaginskiiMK. (2007) [Propolis in treatment of otorhinolaryngological diseases]. Rossiiskaya rinologiya, 2, 55–56 (in Russian).
21.
GreenawayW, ScaysbrookT, WhatleyFR. (1987) The analysis of bud exudate of Populus x euramericana, and of propolis, by gas chromatography-mass spectrometry. Proceedings of the Royal Society, London, B23, 249 – 272.
22.
BankovaV, PopovaM, BogdanovS, SabatiniAG. (2002) Chemical composition of European propolis: expected and unexpected results. Zeitschrift fuer Naturforschung, 57c, 530 – 533.
23.
GreenawayW, ScaysbrookT, WhatleyFR. (1999) The composition and plant origin of propolis: a report of work at Oxford. Bee World, 71, 107–118.
24.
BankovaV, KulevaL., (1989) [Phenolic compounds of propolis from different regions of Bulgaria]. Zhivotnovandi nauki, 26, 94-98 (in Bulgarian).
25.
IsidorovVA, BrzozowskaM, CzyżewskaU, GlinkaL. (2008) Gas chromatographic investigation of phenylpropenoid glycerides from aspen (Populus tremula L.) buds. Journal of Chromatography A, 1198-1199, 196–201.
26.
PopravkoSA, KononenkoGP, TikhomirovaVI, VulfsonNS. (1979) [Secondary metabolites of birch. IV. Identification of groups of flavonoid aglycones in silver birch buds (Betula verrucosa)]. Bioorganichskaya khimiya, 5, 1662–1667 (in Russian).
27.
KononenkoGP, PopravkoSA, VulfsonNS. (1979) [Secondary metabolites of birch. V. Mass spectral method for qualitative analysis of flavonoids aglycones in silver birch buds (Betula verrucosa)]. Bioorganichskaya khimiya, 5, 1836–1842 (in Russian).
28.
WollenweberE. (1975) Flavonoidmuster im Knospenexkret der Betulaceen. Biochemical Systematics and Ecology, 3, 47–52.
29.
KononenkoGP, PopravkoSA, VulfsonNS. (1975) [Flavonoid aglycones of the buds of silver birch (Betula verrucosa)]. Bioorganichskaya khimiya, 1, 506–511 (in Russian).
30.
ZangL-Y, CosmaG, GardnerH, ShiX, CastranovaV, VallyathanV., (2000) Effect of antioxidant protection by p-coumaric acid on low-density lipoprotein cholesterol oxidation. American Journal of Physiology - Cell Physiology, 279, C954-C960.
31.
ItagakiS, KurokawaT, NakataC, SaitoY, OikawaS, KobayashiM, HiranoT, IsekiK., (2009) In vitro and in vivo antioxidant properties of ferulic acid: A comparative study with other natural oxidation inhibitors. Food Chemistry, 114, 466–471.
32.
MetznerJ, BekemeierH, PaintzM, SchneidewindE (1979) On the antimicrobial activity of propolis and propolis constituents. Pharmazie, 34, 97–102.
33.
BurdockGA. (1998) Review of the biological properties and toxicity of bee propolis (propolis). Food & Chemical Toxicology, 36, 347–363.
34.
BanskotaAH, TezukaY, KadotaS., (2001) Recent progress in pharmacological research of propolis. Phytotherapy Research, 15, 561–571.
35.
BankovaV. (2009) Chemical diversity of propolis makes it a valuable source of new biologically active compounds. Journal of ApiProduct & ApiMedical Science, 1, 23 – 28.
36.
WollenweberE. (1988). Occurrence of flavonoid aglycones in medicinal plants. Progress in Clinical Biological Research, 280, 45–55.
37.
AtkinsonP, BlakemanJP. (1982). Seasonal occurrence of an antimicrobial flavanone, sakuranetin, associated with glands of leaves of Ribes nigrum. New Phytologist, 92, 63–74.
LimH, SonKH, ChangHW, BaeKH, KangSS, KimHP. (2008) Anti-inflammatory activity of pectolinarigenin and pectolinarin isolated from Cirsium chanroenicum. Biological & Pharmaceutical Bulletin, 31, 2063–2067.
40.
GunasekeraSP, KinghornAD, CordellGA, FarnsworthNR. (1981) Plant anticancer agents. XIX. Constituents of Aquilaria malaccensis. Journal of Natural Products, 44, 569–572.
41.
BanskotaAH, NagaokaT, SumiokaLY, TezukaY. (2002) Antiproliferative activity of the Netherlands propolis and its active principles in cancer cell lines. Journal of Ethnopharmacology, 80, 67–73.
42.
PopovaM, BankovaV, ButovskaD, PetkovV, Nikolova-DamyanovaB, SabatiniAG, MarcazzanGL, BogdanovS., (2004) Validated methods for the quantification of biologically active constituents of poplar-type propolisPhytochemical Analysis, 15, 235–240.
43.
PopovaM, BankovaV, BogdanovS, TsvetkovaI, NaydenskiC, MarcazzanG-L, SabatiniA.-G. (2007) Chemical characteristics of poplar type propolis of different geographic origin. Apidologie, 38, 306–311.
44.
BonvehiJ, CollF., (1994). Phenolic composition of propolis from China and from South America. Zeitschrift fuer Naturforschung, 49c, 712–718.
