The Effect of Pyridinecarbothioamides on Isoflavonoid Production in Genista tinctoria Cultures in Vitro

Abstract

Previous studies showed that derivatives of pyrazinecarboxylic acid used as elicitors increased either flavonoid or flavonolignan production. The effect of 2-(3-bromobenzylsulfanyl) pyridine-4-carbothioamide on isoflavonoids production in Genista tinctoria culture in vitro was tested. The highest isoflavonoid content (0.3 mg.g⁻¹ DW) in callus culture was found after elicitor application at a concentration of 2.96 × 10⁻⁵ mol.L⁻¹ after 24 h of treatment. The maximum daidzein production (0.2 mg.g⁻¹ DW) was detected after 48 h of elicitor treatment at a concentration of 2.96 × 10⁻³ mol.L-1 and after 24 h of elicitor treatment at a concentration of 2.96 × 10⁻⁵ mol.L⁻¹. Suspension culture produced the highest amount of isoflavonoids (0.3 mg.g⁻¹ DW) after elicitor application at concentrations of 2.96 × 10⁻⁴ mol.L⁻¹ after 24 and 168 h of treatment, and 2.96 × 10⁻⁵ mol.L⁻¹ after 168 h of treatment. The highest content of genistin (0.3 mg. g⁻¹ DW) was detected after 24 h of elicitor treatment at a concentration of 2.96 × 10⁻⁴ mol.L⁻¹. The maximum content of daidzein (3.80 mg.L⁻¹) released into the suspension culture medium was found after elicitor application at a concentration of 2.96 × 10⁻⁴ mol.L⁻¹ after 12 h of treatment.