Marrubium globosum Montbr. et Auch. ex Benth. ssp. libanoticum Boiss. (Lamiaceae) is a medicinal plant used in Lebanon for the treatment of inflammatory diseases, asthma, coughs and other pulmonary and urinary problems. The goal of our study was to assess the biological activity of M. globosum by testing different extracts of the aerial parts for their antiproliferative activity against human melanoma cells using the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay. The chloroform fraction showed the greatest activity. The compounds isolated from the extracts were also tested: the mixture of (13S)-9α,13α-epoxylabda-6β(19),16(15)-diol dilactone and (13R)-9α,13α-epoxylabda-6β(19),16(15)-diol dilactone was the most active fraction, with an IC50 value of 29.2 ± 0.06 μg/mL.
GrassiaA, SenatoreF, ArnoldAN, BrunoM, PiozziF, RiganoD, FormisanoC., (2006) Chemical composition and antimicrobial activity of the essential oils from aerial parts of two Marrubium sp. (Lamiaceae) growing wild in Lebanon. Polish Journal of Chemistry, 80, 623–628.
4.
RiganoD, ArnoldAN, BrunoM, FormisanoC, GrassiaA, PiacenteS, PiozziF, SenatoreF., (2006) Phenolic compounds of Marrubium globosum ssp. libanoticum from Lebanon, Biochemical Systematics and Ecology, 34, 256–258.
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RiganoD, GrassiaA, BorrelliF, AvielloG, PiozziF, BrunoM, ApostolidesNA, CapassoR, SenatoreF., (2006) Phytochemical and pharmacological studies on the acetonic extract of Marrubium globosum ssp. libanoticum. Planta Medica, 72, 575–578.
RiganoD, FormisanoC, BasileA, LavitolaA, SenatoreF, RosselliS, BrunoM., (2007) Antibacterial activity of flavonoids and phenylpropanoids from Marrubium globosum ssp. libanicoticum. Phytotherapy Research, 21, 395–397.
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RiganoD, AvielloG, BrunoM, FormisanoC, RosselliS, CapassoR, SenatoreF, IzzoAA, BorrelliF., (2009) Antispasmodic effects and structure-activity relationships of labdane diterpenoids from Marrubium globosum ssp. libanoticum. Journal of Natural Products, 72, 1477–1481.
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KariotiA, SkopelitiM, TsitsilonisO, HeilmannJ, SkaltsaH., (2007) Cytotoxicity and immunomodulating characteristics of labdane diterpenes from Marrubium cylleneum and Marrubium velutinum. Phytochemistry, 68, 1587–1594.
10.
MohamedAS. (2010) Anticancer activity of Marrubium alysson L. and its phenolic constituents, In Recent Progress in Medicinal Plants, Vol. 27, AwaadAS, GovilJN, SinghVK (Eds). Studium Press LLC. 185–193.
11.
AlkhatibR, JohaS, CheokM, RoumyV, IdziorekT, PreudhommeC, QuesnelB, SahpazS, BailleulF, HennebelleT., (2010) Activity of ladanein on leukemia cell lines and its occurrence in Marrubium vulgare. Planta Medica, 76, 86–87.
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ArgyropoulouA, SamaraP, TsitsilonisO, SkaltsaH., (2012) Polar constituents of Marrubium thessalum Boiss. & Heldr. (Lamiaceae) and their cytotoxic/cytostatic activity. Phytotherapy Research, 26, 1800–1806.
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ZaraiZ, KadriA, Ben ChobbaI, Ben MansourR, BekirA, MejdoubH, GharsallahN., (2011) The in-vitro evaluation of antibacterial, antifungal and cytotoxic properties of Marrubium vulgare L. essential oil grown in Tunisia. Lipids In Health And Disease, 10:161, 1–8.
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MarrelliM, ConfortiF, StattiGA, CachetX, MichelS, TillequinF, MenichiniF., (2011) Biological potential and structure-activity relationships of most recently developed vascular disrupting agents: an overview of new derivatives of natural combretastatin A-4. Current Medicinal Chemistry, 18, 3035–3081.
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ConfortiF, TundisR, MarrelliM, MenichiniF, StattiGA, De CindioB, MenichiniF, HoughtonPJ. (2010) Protective effect of Pimpinella anisoides ethanolic extract and its constituents on oxidative damage and its inhibition of nitric oxide in lipopolysaccharide-stimulated RAW 264.7 macrophages. Journal of Medicinal Food, 13, 137–141.
