Chromolithic Method Development,Validation and System Suitability Analysis of Ultra-Sound Assisted Extraction of Glycyrrhizic Acid and Glycyrrhetinic Acid from Glycyrrhiza glabra
Free accessResearch articleFirst published online August, 2012
Chromolithic Method Development,Validation and System Suitability Analysis of Ultra-Sound Assisted Extraction of Glycyrrhizic Acid and Glycyrrhetinic Acid from Glycyrrhiza glabra
An ultrasound-assisted extraction and chromolithic LC method was developed for simultaneous determination of glycyrrhizic acid (GA) and glycyrrhetinic acid (GL) from the root extract of Glycyrrhiza glabra using RPLC-PDA. The developed method was validated according to the International Conference on Harmonisation. The method exhibited good linearity (r2 >0.9989) with high precision and achieved good accuracies between 97.5 to 101.3% of quantitative results. The method is more sensitive and faster (resolved within ten minutes) than the earlier developed methods using normal LC columns.
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