In this paper we analyzed the antiprotozoal effects of eighteen Cuban propolis extracts (brown, red and yellow type) collected in different geographic areas, using Leishmania amazonensis (as a model of intracellular protozoa) and Trichomonas vaginalis (as a model of extracellular protozoa). All evaluated propolis extracts caused inhibitory effect on intracellular amastigotes of L. amazonensis. However, cytotoxicity on peritoneal macrophages from BALB/c mice was observed. Only five samples decreased the viability of T. vaginalis trophozoites at concentrations lower than 10 μg/mL. No correlation between the type of propolis and antiprotozoal activity was found. Cuban propolis extracts demonstrated activity against both intracellular and extracellular protozoa model, as well as the potentialities of propolis as a natural source to obtain new antiprotozoal agents.
BankovaV., De CastroS.L., MarcucciM.C.Propolis: recent advances in chemistry plant and plant origin. (2000) Apidologie, 31, 3–15; (b) Cuesta-Rubio O, Piccinelli AL, Fernandez MC, Hernández IM, Rosado A, Rastrelli L. (2007) Chemical characterization of Cuban propolis by HPLC-PDA, HPLC-MS, and NMR: the brown, red, and yellow Cuban varieties of propolis. Journal of Agricultural and Food Chemistry, 55, 7502-7509.
2.
De CastroS.L.Propolis: biological and pharmacological activities. Therapeutic uses of this bee-product. (2001) Annal Review of Biomedical Science, 3, 49–83; (b) Duran N, Koc A, Oksuz H, Tamer C, Akaydin Y, Kozlu T, Celik M. (2006) The protective role of topical propolis on experimental keratitis via nitric oxide levels in rabbits. Molecular Cellular Biochemistry, 281, 153-61; (c) Gekker G, Hu S, Spivak M, Lokensgard JR, Peterson PK. (2005) Anti-HIV-1 activity of propolis in CD4(+) lymphocyte and microglial cell cultures. Journal of Ethnopharmacology, 102, 158-63; (d) Sforcin JM, Fernandez A, Lopes CA, Funari SR, Bankova V. (2001) Seasonal effect of Brazilian propolis on Candida albicans and Candida tropicalis. Journal of Venomous Animal Toxins, 7, 139-144; (e) Dantas AP, Salomão K, Barbosa HS, De Castro SL. (2006) The effect of Bulgarian propolis against Trypanosoma cruzi and during its interaction with host cells. Memorias do Instituto Oswaldo Cruz, 101, 207-211; (f) Freitas FS, Shinohara L, Sforcin JM, Guimara S. (2006) In vitro effects of propolis on Giardia duodenalis trophozoites. Phytomedicine, 13, 170–175.
3.
AyresD.C., MarcucciM.C., GiorgioS. (2007) Effects of Brazilian propolis on Leishmania amazonensis. Memorias do Instituto Oswaldo Cruz, 102, 215–20; (b) Machado GM, Leon LL, De Castro SL. (2007) Activity of Brazilian and Bulgarian propolis against different species of Leishmania. Memorias do Instituto Oswaldo Cruz; 102, 73-77; (c) Duran G, Duran N, Culha G, Ozcan B, Oztas H, Ozer B. (2008) In vitro antileishmanial activity of Adana propolis samples on Leishmania tropica: a preliminary study. Parasitology Research, 102, 1217-1225.
4.
XuB.H., ShiM.Z. (2006) An in vitro test of propolis against Trichomonas vaginalis. Zhongguo Ji Sheng Chong Xue Yu Ji Sheng Chong Bing Za Zhi, 24, 477–8; (b) Ledón N, Casacó A, González R, Merino N, González A, Tolón Z. (1996) Efectos antipsoriásico, antiinflamatorio y analgésico del propóleo rojo colectado en Cuba. Revista Cubana Farmacia, 30, 28-32; (c) Cuesta O, Cuellar A, Rojas N, Aquino R, Velez H, Rastrelli L. (1999) A polyisoprenylated benzophenone from Cuban propolis. Journal of Natural Products, 62, 1013-1015; (d) Popolo A, Piccinelli AL, Morello S, Cuesta-Rubio O, Sorrentino R, Rastrelli L, Pinto A. (2009) Antiproliferative activity of Brown Cuban Propolis extract on Human Breast Cancer Cells. Natural Product Communications, 4, 1711-1716.
5.
BaileyM.S., LockwoodD.N.J. (2007) Cutaneous leishmaniasis. Clinics in Dermatology, 25, 203–211; (b) Desjeux P. (2001) The increase in risk factors for leishmaniasis worldwide. Transactions Royal of Society of Tropical Medicine and Hygiene, 95, 239-243.
6.
KhaomekP., IchinoC., IshiyamaA., SekiguchiH., NamatameM., RuangrungsiN., SaifahE., KiyoharaH., OtoguroK., OmuraS., YamadaH. (2008) In vitro antimalarial activity of prenylated flavonoids from Erythrina fusca. Journal Naturat Medicine, 62, 217–220; (b) Sartorelli P, Carvalho CS, Reimão JQ, Ferreira MJ, Tempone AG. (2009) Antiparasitic activity of biochanin A, an isolated isoflavone from fruits of Cassia fistula (Leguminosae). Parasitology Research, 104, 311-314; (c) Khan IA, Avery MA, Burandt CL, Goins DK, Mikell JR, Nash TE, Azadegan A, Walker LA. (2000) Antigiardial activity of isoflavones from Dalbergia frutescens bark. Journal of Natural Products, 63, 1414-1416; (d) Takahashi M, Fuchino H, Sekita S, Satake M, Kiuchi F. (2006) In vitro leishmanicidal constituents of Millettia pendula. Chemical & Pharmaceutical Bulletin, 54, 915-917; (e) Kraft C, Jenett-Siems K, Siems K, Gupta MP, Bienzle U, Eich E. (2000) Antiplasmodial activity of isoflavones from Andira inermis. Journal of Ethnopharmacology, 73, 131-135.
7.
Global Prevalence and incidence of selected curable sexually transmitted infections (World Health Organization, Geneva, 2001), www.who.int/docstore/hiv/GRST/006.htm
8.
MárquezI.H., CampoM., Cuesta-RubioO., PiccinelliA.L., RastrelliL. (2005) Polyprenylated benzophenone derivatives from Cuban propolis. Journal of Natural Products, 68, 931–934; (b) Piccinelli AL, Cuesta-Rubio O, Márquez IH, Campo MF, de Simone F, Rastrelli L. (2005) Isoflavonoids isolated from Cuban propolis. Journal of Agricultural and Food Chemistry, 53, 9010-9016; (c) Márquez IH, Cuesta-Rubio O, Campo MF, Rosado A, Montes de Oca RP, Piccinelli AL, Rastrelli L. (2010) Studies on the constituents of Yellow Cuban Propolis: GC-MS determination of triterpenoids and flavonoids. Journal of Agricultural and Food Chemistry, 58, 4725–4730.
9.
RojasL., FragaJ., SariegoI. (2004) Genetic variability between Trichomonas vaginalis isolates and correlation with clinical presentation. Infection Genetic and Evolution, 4, 5–8; (b) Caio E, Lima D, Kaplan MAC, Nazareth M, Rossi-Bergmann B. (1999) Selective effect of 2′,6′-dihydroxy-4′methoxychalcone isolated from Piper aduncum on Leishmania amazonensis. Antimicrobial Agents and Chemotherapy, 43, 1234-1241; (c) Delorenzi JC, Attias M, Gattass CR, Andrade M, Rezende C, Da Cunha A, Henriques A, Bou-Habib DC, Saraiva BEM. (2001) Antileishmanial activity of an indole alkaloid from Peschiera australis. Antimicrobial Agents and Chemotherapy, 45, 1349-1354; (d) Sladowski D, Steer SJ, Clothier RH, Balls M. (1993) An improve MTT assay. Journal of Immunology Methods, 157, 203-207; (e) Bodley AL, McGarry MW, Shapiro TA. (1995) Drug cytotoxicity assay for African Trypanosomes and Leishmania Species. Journal Infection Diseases, 172, 1157-1159; (f) Tiuman TS, Ueda-Nakamura T, Cortez DAG, Dias Filho BP, Morgado-Díaz JA, de Souza W, Nakamura CV. (2005) Antileishmanial activity of parthenolide, a sesquiterpene lactone isolated from Tanacetum parthenium. Antimicrobial Agents and Chemotherapy, 49, 176-182.
