A simple, sensitive and reliable reversed phase Rapid Resolution Liquid Chromatography (RRLC) method was developed and validated for six biologically active compounds (salidroside, tyrosol, rosarin, rosavin, rosin and rosiridin) in Rhodiola rosea L. roots and powder extracts. The method uses a Phenomenex C18 (2)-HST column at 40°C with a neutral gradient system mobile phase (H2O and acetonitrile), a flow rate of 1.0 mL/min, and UV detection wavelengths set at 205 and 254 nm, simultaneously. Baseline separation of the six active compounds was achieved within 8 minutes. The average percentages of rosavins (rosarin, rosavin, and rosin) in authentic R. rosea roots and root powder extracts were quantitatively determined and a characteristic R. rosea roots RRLC profile was established. The RRLC method is accurate and sensitive; in addition, it effectively increases the sample analysis throughput compared with conventional HPLC.
FillionV.J., SaleemA., RochefortG., AllardM., CuerrierA., ArnasonJ.T. (2008) Phytochemical analysis of Nunavik Rhodiola rosea L. Natural Product Communications, 3, 721–726.
5.
PanossianA., WikmanG., SarrisJ. (2010) Rosenroot (Rhodiola rosea): Traditional use, chemical composition, pharmacology and clinical efficacy. Phytomedicine, 17, 481–493.
6.
ShevtsovV.A., ZholusB.I., ShervarlyV.I., Vol'skijV.B., KorovinY.P., KhristichM.P., RoslyakovaN.A., WikmanG. (2003) A randomized trial of two different doses of a SHR-5 Rhodiola rosea extract versus placebo and control of capacity for mental work. Phytomedicine, 10, 95–105.
7.
MaC., TangJ., WangH., GuX., TaoG. (2008) Simultaneous determination of five active compounds in Rhodiola L. by RP-LC. Chromatographia, 67, 383–388.
LeeM.W., LeeY.A., ParkH.M. (2000) Antioxidative phenolic compounds from roots of Rhodiola sachalinensis. Archives of Pharmacal Research, 23, 455–458.
10.
OhsugiM., FanW., HaseK. (1999) Active-oxygen scavenging activity of traditional nourishing-tonic herbal medicines and active constituents of Rhodiola sacra. Journal of Ethnopharmacology, 67, 111–119.
11.
DubichevA.G., KurkinB.A., ZapesochnayaG.G., VornotzovE.D. (1991) Study of Rhodiola rosea root chemical composition using HPLC. Chemico-Pharmaceutical Journal, 2, 188–193.
12.
GanzeraM., YaylaY., KhanI.A. (2001) Analysis of the marker compounds of Rhodiola rosea L. (golden root) by reversed phase high performance liquid chromatography. Chemical and Pharmaceutical Bulletin, 49, 465–467.
13.
KurkinV.A., ZapesochnayaG.G., GorbunovY.N., NukhimovaskiiE.L., ShreterA.I., ShchavlinskiiA.N. (1986) Chemical investigations on some species of Rhodiola L. and Sedum L. genera and problems of their chemotaxonomy. Rastitel'nye Resursy, 22, 310–319.
14.
Yourui-SuoH.W., YuL.L., JinM.Y., HanQ.W. (2004) Analysis of five pharmacologically active compounds from Rhodiola for natural product drug discovery with capillary electrophoresis. Chromatographia, 60, 589–595.
15.
ShuY.H., XiaoL.H., XingG., Chen-ZhiD.H. (2003) Determination of p-tyrosol and salidroside in three samples of Rhodiola crenulata and one of Rhodiola kirilowii by capillary zone electrophoresis. Analytical and Bioanalytical Chemistry, 377, 370–374.
16.
YuM., YanL., NingY. (2007) Simultaneous determination of salidroside and tyrosol in extracts of Rhodiola L. by microwave assisted extraction and high-performance liquid chromatography. Journal of Pharmaceutical and Biomedical Analysis, 45, 510–515.
17.
TolonenA., PakonenM., HohtolaA., JalonenJ. (2003) Phenylpropanoid glycosides from Rhodiola rosea. Chemical and Pharmaceutical Bulletin, 467–470.
18.
LinhP.T., YoungH.K., HongS.P., JianJ.J., KangJ.S. (2000) Quantitative determination of salidroside and tyrosol from the underground part of Rhodiola rosea by high performance liquid chromatography. Archives of Pharmacal Research, 23, 349–352.
19.
PetsaloA., JalonenJ., TolonenA. (2006) Identification of flavonoids of Rhodiola rosea by liquid chromatography- tandem mass spectrometry. Journal of Chromatography A, 1112, 224–231.
20.
TolonenA., UusitaloJ. (2004) Fast screening method for the analysis of total flavonoids content in plants and foodstuffs by high performance liquid chromatography/electrospray ionization time-of-flight mass spectrometry with polarity switching. Rapid Communications in Mass Spectrometry, 18, 3113–3322.
21.
AvulaB., WangY.H., AliZ., SmillieT.J., FilionV., CuerrierA., ArnasonJ.T., KhanI.A. (2009) RP-HPLC determination of phenylalkanoids and monoterpenoids in Rhodiola rosea and identification by LC-ESI-TO. Biomedical Chromatography, 23, 865–872.
22.
StanevaJ., TodorovaM., NeykovN., EvstatievaL. (2009) Ultrasonically assisted extraction of total phenols and flavonoids from Rhodiola rosea. Natural Product Communications, 4, 935–938.
