Free accessResearch articleFirst published online 2010-12
Comparison of Antibacterial Activity of Natural and Hydroformylated Essential Oil of Thymus capitatus Growing Wild in North Sardinia with Commercial Thymus Essential Oils
Thymus capitatus growing wild in Sardinia showed different essential oil composition if grown surrounding Cagliari than in north Sardinia. Here we verify the composition and antimicrobial activity of the oil to make it suitable for the cosmetic and confectionery industries. With the aim of improving the scent and the antimicrobical activity of T. capitatus essential oil, a hydroformylation reaction was carried out to transform the unsaturated components of the oil into the corresponding aldehydes. The essential oil of T. capitatus exhibited a significant antibacterial activity (MIC 0.125-0.5 mg/mL), and was also found effective on C. albicans (MIC 0.125 mg/mL). After hydroformylation, several new irregular terpenoid aldehydes were detected. The perfume of the new terpenic-like aldehydes is very agreeable and, therefore, the acceptability of the aroma is remarkably improved, but the antimicrobial activity was not increased.
SenatoreF. (1996) Influence of harvesting time on yield and composition of the essential oil of a thyme (Thymus pulegioides L.) growing wild in Campania (southern Italy). Journal of Agriculture and Food Chemistry, 441327-1332.
2.
ArrasG, UsaiM. (2001) Fungitoxic activity of 12 essential oils against four postharvest citrus pathogens: chemical analysis of Thymus capitatus oil and its effect in subatmospheric pressure conditions. Journal of Food Protection, 641025-1029.
3.
CosentinoS, TuberosoCig, PisanoB, SattaM, MasciaV, ArzediE, PalmasF. (1999) In-vitro antimicrobial activity and chemical composition of Sardinian Thymus essential oils. Letters in Applied Microbiology, 29130-135.
4.
De GioannisB, MarongiuB, PorceddaS. (2001)Isolation of Thymus herba-barona volatiles by supercritical extraction. Journal of Essential Oil Research, 13, 240–244.
5.
JulianoCA, MattanaA, UsaiM. (2000) Composition and in vitro antimicrobial activity of the essential oil of Thymus herba-barona Loisel. growing wild in Sardinia. Journal of Essential Oil Research, 12516-522.
6.
PignattiS. (1982) Flora D'Italia. Edagricole, Bologna. Vol. II. 488.
7.
CamardaI, ValsecchiF. (1990) Piccoli arbusti, liane e suffrutici spontanei della Sardegna. Carlo Delfino, Sassari. 211.
8.
SolinasV, GessaC, FalchiDelitala L. (1981) High-performance liquid chromatographic analysis of carvacrol and thymol in the essential oil of Thymus capitatus. Journal of Chromatography A, 219332-337.
9.
ArrasG, GrellaGE. (1992) Wild thyme, Thymus capitatus, essential oil seasonal changes and antimycotic activity. The Journal of Horticultural Science and Biotechnology, 67197-202.
10.
ArrasG, PigaA, D'HallewinG. (1994) Effectiveness of Thymus capitatus aerosol application at subatmospheric pressure to control green mold on ‘Avana’ mandarin fruit. Acta Horticulturae (ISHS)International Symposium on Postharvest Treatment of Horticultural Crops, 368382-386.
11.
ArrasG, AgabbioM, PigaA, D'hallewinG. (1995) Fungicide effect of volatile compounds of Thymus capitatus essential oil. Acta Horticulturae, 379593-600.
12.
UsaiM. (2005) Le piante spontanee della Sardegna: Thymus capitatus (L.) Hoffmanns. et Link e Salvia desoleana Atzei e Picci preziosi contenitori di principi biologicamente attivi. Natural1, 47, 72-74.
13.
AtzeiAD. (2003) Le piante nella tradizione popolare della Sardegna. Carlo Delfino, Sassari. 219.
14.
Farmacopea Ufficiale Italiana. (1999) 10th Ed., Droghe vegetali e preparazioni. Istituto Poligrafico e Zecca dello Stato. Roma.
15.
DugoG, LicandroG, CrotoneoA, DugoG. (1983) Sulla genuinità delle essenze agrumarie. Nota I. Caratterizzazione di essenze di limone Siciliane. Essenze e Derivati Agrumari, 53, 173-217.
16.
AdamsRP. (2001) Identification of Essential Oil Components by Gas Chromatography/Quadrupole Mass Spectroscopy. U.S.A. IL, Allured Pub. Corp., Carol Stream.
17.
DaviesNW. (1990) Gas chromatographic retention indices of monoterpenes and sesquiterpenes on methyl silicon and Carbowax 20M phases. Journal of Chromatography A, 5031-24.
18.
HellerSR, MilneGwa. (1983) EPA/NIH mass spectral data base. Washington, DC, U.S. Government Printing Office.
19.
JenningsWG, ShibamotoT. (1980) Qualitative analysis of flavor and fragrance volatiles by glass capillary gas chromatography. New York, Academic Press.
20.
McLaffertyFW, StaufferDB. (1989) The Wiley NBS registry of mass spectral data. John Wiley and Sons, New York.
21.
StenhagenE, AbrahamssonS, McLaffertyFW. (1974) Registry of mass spectral data. John Wiley and Sons, New York.
22.
MarchettiM, SechiB, UsaiM. (2004) Idroformilazione di oli essenziali: un metodo di valorizzazione di essenze agrumarie di scarto. La Chimica e L’ Industria, 8652-56.
23.
PaganelliS, SpanoL, MarchettiM, PiccoloO. (2005) Synthesis of fragrances through safrole and isosafrole hydroformylation. La Chimica e L'Industria, 8794-98.
24.
PaganelliS, CiappaA, MarchettiM, ScrivantiA, MatteoliU. (2006) Hydroformylation of m-diisopropenylbenzene and 1-isopropyl-3-isopropenylbenzene for the preparation of the fragrance Florhydral®. Journal of Molecular. Catalysis A: Chemical, 247138-144.
