Phytochemical investigations of the stems of Tinospora crispa led to the isolation of one new aporphine alkaloid, N-formylasimilobine 2-O-β-D-glucopyranoside (1), along with six known alkaloids, N-formylasimilobine 2-O-β-D-glucopyranosyl-(1→2)-β-D-glucopyranoside (2) (tinoscorside A), N-formylanonaine (3), N-formyldehydroanonaine (4), N-formylnornuciferine (5), N-demethyl-N-formyldehydronornuciferine (6), magnoflorine (7), paprazine (8), N-trans-feruloyltyramine (9), and cytidine (10). The structure of new compound 1 was deduced on the basis of spectroscopic data.
Herbal Medicine Research Group. (2002) Compendium of Medicinal Plants Used in Malaysia; Kuala Lumpur, Institute for Medical Research, Malaysia, 2392-393.
2.
PerryLM. (1980) Medicinal Plants of East and South East Asia; MIT Press, Cambridge, Massachusetts. 268.
3.
SiddiqiMA, NasirE, AliSI. (1974) Flora of West Pakistan; Ferozsons, Karachi, 745.
4.
MauryaR, WazirV, KapilRS. (1994) Biotherapeutic diterpene glycosides from Tinospora species. Journal of Indian Chemical Society, 71361-364.
5.
SinghSS, PandeySC, SrivastavaS, GuptaVS, PatroB, GhoshAC. (2003) Chemistry and medicinal properties of Tinospora cordifolia (Guduchi). Indian Journal of Pharmacology, 3583-91.
6.
StévignyC, BaillyC, Quetin-LeclercqJ. (2005)Cytotoxic and antitumor potentialities of aporphinoid alkaloids. Current. Medicinal Chemistry-Anticancer Agents, 5173-182.
7.
ChoudharyMI, IsmailM, ShaariK, AbbaskhanA, SattarSA, LajisNH, Atta-ur-Rahman (2010) cis-Clerodane-type furanoditerpenoids from Tinospora crispa. Journal of Natural Products, 73541-547.
8.
SariA, GrayAI, SariyarG. (2004) A new dehydroaporphine alkaloid from Papaver fugax. Natural Product Research, 18265-268.
9.
RufinoAR, BrantAjc, SantosJbo, FerreiraMjp, EmerencianoVP. (2005) Simple method for identification of skeletons of aporphine alkaloids from 13C NMR data using artificial neural networks. Journal of Chemical Information and Modelling, 45645-651.
10.
ShammaM. (1972) The Isoquinoline Alkaloids. Academic Press, New York. 208-209.
11.
KiemPV, MinhCV, DatNT, KinhLV, HangDT, NamNH, CuongNX, HuongHT, LauTV. (2010) Aporphine alkaloids, clerodane diterpenes, and other constituents from Tinospora cordifolia. Fitoterapia, doi:10.1016/j.fitote.2010.01.005.
12.
PachalyP, AdnanAZ, WillG. (1992) Zur NMR-spektroskopie von N-acylaporphin-alkaloiden aus Tinospora crispa. Planta Medica, 58184-187.
13.
MenacheryMD, BlakeGW, BeiswengerC, FreyerA. (1995) Alkaloids from the neutral fraction of Telitoxicum krukovii. Heterocycles, 411425-1430.
14.
NozakaT, MorimotoI, IshinoM, OkiysuT, KondohH, KyogokuK, SugawaraY, IwasakiH. (1987) Mutagenic principles in Sinomeni caulis et rhizome. 1. The structure of a mutagenic alkaloid, N-demethyl-N-formyldehydronornuciferine, in the neutral fraction of the methanolic extract. Chemical & Pharmaceutical Bulletin, 352844-2848.
15.
Barbosa-FilhoJM, Da-CunhaEvl, CornelioML, DiasCds, GrayAI. (1997) Cissaglaberrimine, an aporphine alkaloid from Cissampelos glaberrima. Phytochemistry, 44959-961.
FukudaN, YonemitsuM, KimuraT. (1983) Studies on the constituents of the stem of Tinospora tuberculata (Beumee). Chemical & Pharmaceutical Bulletin, 31156-161.
18.
DekeboA, KashiwagiT, TebayashiS, KimC. (2007) Nitrogenous ovipositional deterrents in the leaves of sweet pepper (Capsicum annuum) at the mature stage against the leaf miner, Liriomyza trifolii (Burgess). Bioscience Biotechnology & Biochemistry, 71421-426.
19.
KamerlingJP, GerwigGJ, VliegenthartJfg, ClampJR. (1975)Characterization by gas-liquid chromatography-mass spectrometry and proton-magnetic resonance spectroscopy of pertrimethylsilyl methyl glycosides obtained in the methanolysis of glycoproteins and glycopeptides. Biochemical Journal, 151491-495.
