Natural plant extracts often contain compounds that are useful in pest management applications. The essential oil of Eupatorium capillifolium (dog-fennel) was investigated for antifungal and insecticidal activities. Essential oil obtained by hydrodistillation of aerial parts was analyzed by gas chromatography (GC) and gas chromatography–mass spectrometry (GC/MS). The major components were determined to be thymol methyl ether (=methyl thymol) (36.3%), 2,5-dimethoxy-p-cymene (20.8%) and myrcene (15.7%). Antifungal activity of the essential oil was weak against the plant pathogens Colletotrichum acutatum, C. fragariae, and C. gloeosporioides in direct bioautography assay. The E. capillifolium oil showed promising repellent activity against the yellow fever mosquito Aedes aegypti, whereas the oil exhibited moderate activity against the mosquito's first instar larvae in a high throughput bioassay. Topical applications of the oil showed no activity against the blood-feeding female adults of A. aegypti. Eupatorium capillifolium essential oil showed a linear dose response between adult lace bug (Stephanitis pyrioides) mortality and increasing oil concentration in an adulticidal activity bioassay. The dog-fennel oil was more potent than the conventional insecticide malathion. In conclusion, these combined results showed Eupatorium capillifolium oil is a promising novel source of a biological insecticide with multiple modes of action.
CarterSW. (1989) A review of the use of synthetic pyrethroids in public health and vector pest control. Journal of Pest Science, 27, 361–374.
2.
KolaczinskiJH, CurtisCF. (2004) Chronic illness as a result of low-level exposure to synthetic pyrethroid insecticides. Food and Chemical Toxicology, 42, 697–706.
3.
IsmanMB. (2000) Plant essential oils for pest and disease management. Crop Protection, 19, 603–608.
4.
SilvaWJ, DoriaGAA, MaiaRT, NunesRS, CarvalhoGA, BlankAF, AlvesPB, MarcalRM, CavalcantiSCH. (2008) Effects of essential oils on Aedes aegypti larvae: Alternatives to environmentally safe insecticides. Bioresource Technology, 99, 3251–3255.
5.
SchmidtGJ, SchillingEE. (2000) Phylogeny and biogeography of Eupatorium (Asteraceae: Eupatorieae) based on nuclear ITS sequence data. American Journal of Botany, 87, 716–726.
6.
KingRM, RobinsonH. (1987) The genera of Eupatorieae (Asteraceae). Monographs in systematic botany, Missouri Botanical Garden, 22, 1–581.
7.
TimmeSL. (2007) Wildflowers of Mississippi. University Press of Mississippi, Jackson, Mississippi, 74–76.
ClavinML, GorzalczanyS, MinoJ, CarinaKadarian C, MartinoV, FerraroG, AcevedoC. (2000) Antinociceptive effect of some Argentine medicinal species of Eupatorium. Phytotherapy Research, 14, 275–277.
11.
AbadMJ, BermejoP S.SanchezPalomino S, ChiribogaX, CarrascoL. (1999) Antiviral activity of some South American medicinal plants. Phytotherapy Research, 13, 142–146.
12.
ChevallierA. (1996) The encyclopedia of medicinal plants. DK Publishing Inc., New York, 206–207.
13.
HabtemariamS. (2001) Antiinflammatory activity of the antirheumatic herbal drug, gravel root (Eupatorium purpureum): Further biological activities and constituents. Phytotherapy Research, 15, 687–690.
14.
ZouFC, DuanG, XieYJ, ZhouY, DongGD, LinRQ, ZhuXQ. (2009) Molluscicidal activity of the plant Eupatorium adenophorum against Oncomelania hupensis, the intermediate host snail of Schistosoma japonicum. Annals of Tropical Medicine and Parasitology, 103, 549–553.
YankanchiSR, PatilSR. (2009) Field efficacy of plant extracts on larval populations of Plutella xylostella L. and Helicoverpa armigera Hub. and their impact on cabbage infestation. Journal of Biopesticides, 2, 32–36.
17.
RuffinengoS, EguaraM, FlorisI.FaverinC, BailacP, PonziM. (2005) LD50 and repellent effects of essential oils from Argentinean wild plant species on Varroa destructor. Journal of Economic Entomology, 98, 651–655.
18.
MacedoME, ConsoliRAGB, GrandiTSM, AnjosAMG dos, OliveiraAB de, MendesNM, QueirozRO, ZaniCL. (1997) Screening Asteraceae (Composite) plant extracts for larvicidal activity against Aedes fluviatilis (Diptera: Culicidae). Memórias do Instituto Oswaldo Cruz, 92, 565–570.
19.
PinoJA, RosadoA, FuentesV. (1998) Essential oil of Eupatorium capillifolium (Lam.) Small from Cuba. Journal of Essential Oil Research, 10, 79–80.
20.
DomínguezXA, GomezME, GomezPA, VillarrealAN, RomboldC. (1971) Physical data on the essential oils of five Composite plants. Planta Medica, 19, 52–54.
21.
WangX, WedgeDE, TabancaN, JohnsonRD, CutlerSJ. PaceP, SmithBJ, ZhouL. (2008) Development of A miniaturized 24-well strawberry leaf disk bioassay for evaluating natural fungicides. Natural Product Communications, 3, 1079–1084.
22.
HoelD, PridgeonJW, BernierUR, ChauhanK, MeepagalaK, CantrellC. (2010) Departments of Defense and Agriculture team up to develop new insecticides for mosquito control. Wingbeats, 21, 19–34.
23.
KatritzkyAR, WangZ, SlavovS, TsikoliaM, DobchevD, AkhmedovNG, HallCD, BernierUR, ClarkGG, LinthicumKJ. (2008) Synthesis and bioassay of novel mosquito repellents predicted from chemical structure. Proceedings of the National Academy of Sciences, 105, 7359–7364.
24.
BernierUR, FurmanKD, KlineDL, AllanSA, BarnardDR. (2005) Comparison of contact and spatial repellency of catnip oil and N,N-Diethyl-3-methylbenzamide (Deet) against mosquitoes. Journal of Medical Entomology, 42, 306–311.
25.
SampsonBJ, TabancaN, KirimerN, DemirciB, BaserKHC, KhanIA, SpiersJM, WedgeDE. (2005) Insecticidal activity of 23 essential oils and their major compounds against adult Aphis pseudobrassicae (Davis) (Aphididae: Homoptera). Pest Management Science, 61, 1122–1128.
26.
KoenigWA, JoulainD, HochmuthDH. (2004) Terpenoids and related constituents of essential oils. MassFinder 3. Ed. HochmuthD.H.Convenient and Rapid Analysis of GCMS, incorporating, Hamburg, Germany.
27.
McLaffertyFW, StaufferDB. (1989) The Wiley/NBS registry of mass spectral data.J. Wiley and Sons, New York.
28.
JoulainD, KoenigWA. (1998) The atlas of spectra data of sesquiterpene hydrocarbons.E.B.Verlag, Hamburg.
29.
ESO 2000. (1999) The complete database of essential oils.Boelens Aroma Chemical Information Service, The Netherlands.
30.
JenningsWG, ShibamotoT. (1980) Quantitative analysis of flavor and fragrance volatiles by glass capillary GC.Academic Press, New York.
31.
TabancaN, WedgeDE, WangX, DemirciB, BaserKHC, ZhouL, CutlerSJ. (2008) Chemical composition and antifungal activity of Angelica sinensis essential oil against three Colletotrichum species. Natural Product Communications, 3, 1073–1078.
32.
PoseyKH, SchreckCE. (1981) An airflow apparatus for selecting female mosquitoes for use in repellent and attraction studies. Mosquito News, 41, 566–568.
33.
PridgeonJW, BecnelJJ, ClarkGG, LinthicumKJ. (2009) A high throughput screening method to identify potential pesticides for mosquito control. Journal of Medical Entomology, 46, 335–341.
34.
PridgeonJW, PereiraRM, BecnelJJ, AllanSA, ClarkGG, LinthicumKJ. (2008) Susceptibility of Aedes aegypti, Culex quinquefasciatus Say, and Anopheles quadrimaculatus Say to 19 pesticides with different modes of action. Journal of Medical Entomology, 45, 82–87.
35.
SampsonBJ, McLaughlinJL, WedgeDE. (2003) Evaluating the extract of the Paw Paw tree as a botanical insecticide. Arthropod Management Tests, 28, L5.
36.
WedgeDE, TabancaN, SampsonBJ, WerleC, DemirciB, BaserKHC, NanP, DuanP, LiuZ. (2009) Antifungal and insecticidal activity from two Juniperus essential oils. Natural Product Communications, 4, 123–127.
