Inula oculus-christi L. (Compositae) extract was chromatographed and three sesquiterpene lactones ergolide, gaillardin and pulchellin C were isolated. The structures of these compounds were determined by analysis of their spectroscopic data, and their crystal structures were defined using X-ray crystallography; the isolation of ergolide and pulchellin C is reported for the first time from this species. These three compounds were evaluated for their in vitro cytotoxic activity against MDBK, MCF7 and WEHI164 cells; ergolide and gaillardin exhibited lower and significantly different IC50 values compared with pulchellin C (p<0.001).
YangC, WangCM, JiaZJ. (2003) Sesquiterpenes and other constituents from the aerial parts of Inula japonica. Planta Medica, 69, 662–666.
5.
LiuS, LiuH, YanW, ZhangL, BaiN, HoCT. (2004) Studies on 1-O-acetylbritannilactone and its derivative, (2-O-butyloxime-3-phenyl)-propionyl-1-O-acetylbritannilactone ester. Bioorganic & Medicinal Chemistry Letters, 14, 1101–1114.
6.
SongYJ, LeeDY, KimSN, LeeKR, LeeHW, HanJW, KangDW, LeeHY, KimYK. (2005) Apoptotic sesquiterpene lactone ergolide through the inhibition of NF-kB signaling pathway. Journal of Pharmacy and Pharmacology, 57, 1591–1597.
7.
ChenCN, HuangHH, WuCL, LinCP, HsuJT, HsiehHP, ChuangSE, LaiGM. (2007) Isocostunolide, a sesquiterpene lactone, induces mitochondrial membrane depolarization and caspase-dependent apoptosis in human melanoma cells. Cancer Letters, 246, 237–252.
8.
XieHG, ChenH, CaoB, ZhangHW, ZouZM. (2007) Cytotoxic germacranolide sesquiterpene from Inula cappa. Chemical & Pharmaceutical Bulletin (Tokyo), 55, 1258–60.
9.
MosaddeghM, OstadSN, NaghibiF, HamzelooMoghadam M. (2006) Cytotoxic effects of five species of Inula against some tumor cell lines. Iranian Journal of Pharmaceutical Sciences, 2, 203–208.
10.
VajsV, NevešćaninM, MacuraS, JuranićN, MonecovićN, MilosavlijevićS. (2003) Sesquiterpene lactones from aerial parts of Inula oculus-christi. Fitoterapia, 74, 508–510.
11.
SchagenJD, StraverL, van MeursF, WilliamsG. (1989) CAD4 Version 5.0, Delft Instrument X-ray diffraction.
12.
RaeAD. (1996) RAELS. A comprehensive Constrained Least Squares Refinement Program, University of New University of New South Wales.
13.
JohnsonC. (1976) ‘ORTEP-II', Oak Ridge National Laboratory, Tennessee, USA.
RustaiyanA, ZareK, BinyazT, FazlalizadehG (1989) A seco-guaianolide and other sesquiterpene lactones fromPostia bombycina, Phytochemistry, 29, 3127–3129.
16.
GonzalezRomero MA, VillaescusaCastillo L, DiazLanza AM, BartolomeEsteban C, FernandezMatellano L. (2001) Phytochemistry and pharmacological studies of Inula montana L. Recent Research Developments in Phytochemistry, 5, 255–268.
17.
AlleyMC, ScudieroDA, MonkesA, HurseyML, CzerwinskiM J, FineD L, AbbottB J, MayoJG, ShoemakerRH, BoydMR. (1988) Feasibility of drug screening with panel of human tumor cell lines using a microculture tetrazolium assay. Cancer Research, 48, 589–601.