The essential oils from Foeniculum vulgare commercial aerial parts and fruits were isolated by hydrodistillation, with different distillation times (30 min, 1 h, 2 h and 3 h), and analyzed by GC and GC-MS. The antioxidant ability was estimated using four distinct methods. Antibacterial activity was determined by the agar diffusion method. Remarkable differences, and worrying from the quality and safety point of view, were detected in the essential oils. trans-Anethole (31-36%), α-pinene (14-20%) and limonene (11-13%) were the main components of the essentials oil isolated from F. vulgare dried aerial parts, whereas methyl chavicol (= estragole) (79-88%) was dominant in the fruit oils. With the DPPH method the plant oils showed better antioxidant activity than the fruits oils. With the TBARS method and at higher concentrations, fennel essential oils showed a pro-oxidant activity. None of the oils showed a hydroxyl radical scavenging capacity >50%, but they showed an ability to inhibit 5-lipoxygenase. The essential oils showed a very low antimicrobial activity. In general, the essential oils isolated during 2 h were as effective, from the biological activity point of view, as those isolated during 3 h.
MuckensturmB, FoechterlenD, ReduronJ-P, DantonP, HildenbrandM. (1997) Phytochemical and chemotaxonomic studies of Foeniculum vulgare. Biochemical Systematics and Ecology, 25, 353–358.
2.
CoelhoJAP, PereiraAP, MendesRL, PalavraAMF. (2003) Supercritical carbon dioxide extraction of Foeniculum vulgare volatile oil. Flavour and Fragrance Journal, 18, 316–319.
3.
OktayM, GülçinI, KüfrevioğrluÖI. (2003) Determination of in vitro antioxidant activity of fennel (Foeniculum vulgare) seed extracts. LWT-Food Science and Technology, 36, 263–271.
4.
DamjanovićB, LepojevićŽ, ŽivkovićV, TolićA. (2005) Extraction of fennel (Foeniculum vulgare Mill.) seeds with supercritical CO2: Comparison with hydrodistillation. Food Chemistry, 92, 143–149.
5.
ÖzcanM, ChalchatJC. (2006) Effect of collection time on chemical composition on the essential oil of Foeniculum vulgare subsp. piperitum growing wild in Turkey. European Food Research and Technology, 224, 279–281.
6.
Mímica-DukićN, KujundžićS, SokovićM, CouladisM. (2003) Essential oil composition and antifungal activity of Foeniculum vulgare Mill. obtained by different distillation conditions. Phytotherapy Research, 17, 368–371.
7.
ZellerA, RychlikM. (2006) Character impact odorants of fennel fruits and fennel tea. Journal of Agricultural and Food Chemistry, 54, 3686–3692.
8.
BarazaniO, CohenY, FaitA, DiminshteinS, DudaiN, RavidU, PutievskyE, FriedmanJ. (2002) Chemotypic differentiation in indigenous populations of Foeniculum vulgare var. vulgare in Israel. Biochemical Systematics and Ecology, 30, 721–731.
9.
RavidU, PutievskyE, SnirN. (1983) The volatile components of oleoresins and the essential oils of Foeniculum vulgare in Israel. Journal of Natural Products, 6, 848–851.
10.
CavaleiroCMF, RoqueOL, da CunhaAP. (1993) Contribution for the characterization of Portuguese fennel chemotypes. Journal of Essential Oil Research, 5, 223–225.
11.
MarottiM, PiccagliaR, GiovanelliE, DeansSG, EagleshamE. (1994) Effects of variety and ontogenic stage on the essential oil composition and biological activity of fennel (Foeniculum vulgare Mill.). Journal of Essential Oil Research, 6, 57–62.
12.
BernáthJ, NémethÉ, KattaaA, HéthelyiÉ. (1996) Morphological and chemical evaluation of fennel (Foeniculum vulgare Mill.) populations of different origin. Journal of Essential Oil Research, 8, 247–253.
13.
BarazaniO, FaitA, CohenY, DiminshteinS, RavidU, PuticvskyE, LewinshnE, FriedmanJ. (1999) Chemical variation among indigenous populations of Foeniculum vulgare var. vulgare in Israel. Planta Medica, 65, 486–489.
14.
MiraldiE. (1999) Comparison of the essential oils from ten Foeniculum vulgare Miller samples of fruits of different origin. Flavour and Fragrance Journal, 14, 379–382.
15.
Garcia-JiménezN, Pérez-AlonsoMJ, Velasco-NegueruelaA. (2000) Chemical composition of fennel oil, Foeniculum vulgare, Miller from Spain. Journal of Essential Oil Research, 12, 159–162.
16.
PiccagliaR, MarottiM. (2001) Characterization of some Italian types of wild fennel (Foeniculum vulgare Mill.). Journal of Agricultural and Food Chemistry, 49, 239–244.
17.
YaminiY, SefidkonF, PourmotazaviSM. (2002) Comparison of essential oil composition of Iranian fennel (Foeniculum vulgare) obtained by supercritical carbon dioxide extraction and hydrodistillation methods. Flavour and Fragrance Journal, 17, 345–348.
18.
ÖzcanMM, ChalchatJC, ArslanD, AteşA, ünverA. (2006) Comparative essential oil composition and antifungal effect of bitter fennel (Foeniculum vulgare ssp. piperitum) fruit oils obtained during different vegetation. Journal of Medicinal Food, 9, 552–561.
19.
RubertoG, BarattaMT, DeansSG, DormanHJD. (2000) Antioxidant and antimicrobial activity of Foeniculum vulgare and Crithmum maritimum essential oils. Planta Medica, 66, 687–693.
20.
Puertas-MejíaM, HillebrandS, StashenkoE, WinterhalterP. (2002) In vitro radical scavenging activity of essential oils from Columbian plants and fractions from oregano (Origanum vulgare L.) essential oil. Flavour and Fragrance Journal, 17, 380–384.
21.
SinghG, KapoorIPS, PandeySK, SinghUK, SinghRK. (2002) Studies of essential oils: part 10; Antibacterial activity of volatile oils of some spices. Phytotherapy Research, 16, 680–682.
22.
StashenkoEE, PuertasMA, MartínezJR. (2002) SPME determination of volatile aldehydes for evaluation of in-vitro antioxidant activity. Analytical and Bioanalytical Chemistry, 373, 70–74.
23.
CantorePL, IacobellisNS, de MarcoA, CapassoF, SenatoreF. (2004) Antibacterial activity of Coriandrum sativum L. and Foeniculum vulgare Miller var. vulgare (Miller) essential oils. Journal of Agricultural and Food Chemistry, 52, 7862–7866.
24.
DadalioğrluI, EvrendilekGA. (2004) Chemical compositions and antibacterial effects of essential oils of Turkish oregano (Origanum minutiflorum), bay laurel (Laurus nobilis), Spanish lavender (Lavandula stoechas L.), and fennel (Foeniculum vulgare) on common foodborne pathogens. Journal of Agricultural and Food Chemistry, 52, 8255–8260.
25.
ErtürkÖ, ÖzbucakTBBayrakA. (2006) Antimicrobial activities of some medicinal essential oils. Herba Polonica, 52, 58–66.
26.
PoliteoO, JukićM, MilošM. (2006) Chemical composition and antioxidant activity of essential oils of twelve spice plants. Croatica Chemica Acta, 79, 545–552.
27.
MataAT, ProençaC, FerreiraAR, SerralheiroMLM, NogueiraJMF, AraújoMEM. (2007) Antioxidant and antiacetylcholinesterase activities of five plants used as Portuguese food spices. Food Chemistry, 103, 778–786.
28.
PortuguesaFarmacopeia (1995) Monografias do funcho amargo e doce, fruto. (8th ed.) Instituto Nacional da Farmácia e do Medicamento (INFARMED), Lisboa, Portugal, 1995, pp. 2141
29.
MassoudH. (1992) Study on the essential oils in seeds of some fennel cultivars under Egyptian environmental conditions. Planta Medica, 58, A681–A682.
30.
GuillénMD, ManzanosMJ. (1996) A study of several parts of the plant Foeniculum vulgare as a source of compounds with industrial interest. Food Research International, 29, 85–88.
31.
KrügerH, HammerK. (1999) Chemotypes of fennel (Foeniculum vulgare Mill.)Journal of Essential Oil Research, 11, 79–82.
32.
HuangD, OuB, PriorRL. (2005) The chemistry behind antioxidant capacity assays. Journal of Agricultural and Food Chemistry, 53, 1841–1856.
33.
Sánchez-MorenoC, LarrauriJA, Saura-CalixtoF. (1998) A procedure to measure the antiradical efficiency of polyphenols. Journal of the Science and Food Agriculture, 76, 270–276.
34.
RubertoG, BarattaMT. (2000) Antioxidant activity of selected essential oil components in two lipid model systems. Food Chemistry, 69, 167–174.
35.
HinneburgI, DormanHJD, HiltunenR. (2006) Antioxidant activities of extracts from selected culinary herbs and spices. Food Chemistry, 97, 122–129.
36.
LourensACU, ReddyD, BaserKHC, ViljoenAM, van VuurenSF. (2004) In vitro biological activity and essential oil composition of four indigenous South African Helichrysum species. Journal of Ethnopharmacology, 95, 253–258.
37.
BurtS. (2004) Essential oils: their antibacterial properties and potential applications in foods – a review. International Journal of Food Microbiology, 94, 223–253.
38.
Council of Europe (COE) (2007) European Directorate for the Quality of Medicines. European Pharmacopoeia 6th Edition. Strasbourg.
39.
BloisMS. (1958) Antioxidant determinations by the use of a stable free radical. Nature, 181, 1199–1200.
40.
DormanHJD, DeansSG, NobleRC. (1995) Evaluation in vitro of plant essential oils as natural antioxidants. Journal of Essential Oil Research, 7, 645–651.
41.
BarattaMT, DormanHJD, DeansSG, BiondiDM, RubertoG. (1998) Chemical composition, antimicrobial and antioxidative activity of laurel, sage, rosemary, oregano and coriander essential oils. Journal of Essential Oil Research, 10, 618–627.
42.
ChungSK, OsawaT, KawakishiS. (1997) Hydroxyl radical scavenging effects of species and scavengers from brown mustrad (Brassica nigra). Bioscience, Biotechnology and Biochemistry, 61, 118–123.
43.
FrumY, VilijoenA. (2006) In vitro 5-lipoxygenase activity of three indigenous South African aromatic plants used in traditional healing and the stereospecific activity of limonene in the 5-lipoxygenase assay. Journal of Essential Oil Research, 18, 85–88.
44.
FaleiroML, MiguelMG, LadeiroF, VenâncioF, TavaresR, BritoJC, FigueiredoAC, BarrosoJG, PedroLG. (2003) Antimicrobial activity of essential oils isolated from Portuguese endemic species of Thymus. Letters in Applied Microbiology, 36, 35–40.
