The chemical composition of the essential oil from peppermint (Mentha x piperita L.) was analyzed by GC/FID and GC-MS. The main constituents were menthol (40.7%) and menthone (23.4%). Further components were (+/-)-menthyl acetate, 1,8-cineole, limonene, β-pinene and β-caryophyllene. Peppermint oil possessed antiradical activity with respect to DPPH (diphenyl picryl hydrazyl) and hydroxyl (OH•) radicals, exercising stronger antioxidant impact on the OH• radical. The concentrations required for 50% inhibition of the respective radical (IC50) were 860 μg/mL for DPPH and 0.26 μg/mL for OH•. Peppermint essential oil demonstrated antioxidant activity in a model linoleic acid emulsion system in terms of inhibiting conjugated dienes formation by 52.4% and linoleic acid secondary oxidized products generation by 76.9% (at 0.1% concentration).
EvansRJ, ReyhoutGS. (1992) Alternates to synthetic antioxidants. In: Science and Human Nutrition, CharalambousG. (Ed). Elsevier Science, Amsterdam, NL.27.
2.
PokornyJ. (1991) Natural antioxidants for food use. Trends in Food Science and Technology, 2, 223–227.
3.
GordonMH, WengXC. (1992) Antioxidant properties of extracts from tanshen (Salvia miltriorrhiza Bunge). Food Chemistry, 44, 119–122.
4.
KimSY, KimJH, KimSK, OhMJ, JungMY. (1994) Antioxidant activities of selected oriental herb extracts. Journal of the American Oil Chemists Society, 71, 633–640.
5.
PolitiM, RodriguesC, GiãoM, PintadoM, CastroP. (2008) Antioxidant principles and volatile constituents from the Northwestern Iberian mint “erva-peixeira”, Mentha cervina. Natural Product Communications, 3, 2065–2068.
6.
MoscianoG, FasanoM, CassidyJ, ConnellyK, MazeikoP, MontenegroA. (1993) Organoleptic characteristics of flavor materials. Perfumer and Flavorist, 18, 38–41.
7.
KavrayanD, AqydemirT. (2001) Partial purification and characterization of polyphenoloxidase from peppermint (Mentha x piperita). Food Chemistry, 74, 147–154.
8.
Moldao-MartinsM, Beirao-da-CostaS, NevesC, CavalieroC, SalguerioL, Beirao-da-CostaML. (2004) Olive oil flavored by the essential oils of Mentha x piperita and Thymus mastichina L. Food Quality and Preference, 15, 447–452.
LawrenceB.Essential Oils 1995– 2000. (2003). Allured Publishing Corporation, Carol Stream. USA.
12.
LawrenceB. (1993) Progress in essential oils. Perfumer and Flavorist, 18, July/August, 59–72.
13.
LooA, RichardH. (1992) Origine et proprietes des epices et des aromates bruts. In: RichardH. (Ed), Eoices et Aromatespp. 18-22, Nature, Paris, France: TEC and DOC – Lavoisier.
14.
AdamsRP. (2001) Identification of Essential Oil Components by Gas Chromatography/Quadrupole Mass Spectroscopy.Allured, Illinois.
15.
DaviesNW. (1990) Gas chromatographic retention indices of monoterpenes and sesquiterpenes on methyl silicone and Carbowax 20M phases. Journal of Chromatography, 503, 1–24.
16.
JenningsW, ShibamotoT. (1980) Qualitative analysis of flavour and fragrance volatiles by glass capillary gas chromatography.Academic Press, New York, NY.
17.
JoulainD, KönigWA. (1998) The Atlas of Spectral Data of Sesquiterpene Hydrocarbons.E.B.-Verlag, Hamburg.
18.
KondjoyanN, BerdaquéJ-L. (1996) A compilation of relative retention indices for the analysis of aromatic compounds.Edition du Laboratoire Flaveur. Saint Genes Champanelle
19.
ArctanderS. (1969) Perfume and flavour chemicals (aroma chemicals).Arctander Publishing, Montclair.
20.
BauerK, GarbeD, SurburgH. (1997) Common fragrance and flavor materials, 3rd Ed., Wiley-VCH Verlag GmbH, Weinheim.
AruomaO, LaughtonM, HalliwellB. (1989) Carnosin, homocarnosin and anserine: Could they act as antioxidants in vivo. Biochemistry Journal, 264, 863–869.
24.
HalliwellB, GutteridgeJ, AruomaO. (1987) The deoxyribose method: A simple “test tube” assay for determination of rate constants for reaction of hydroxyl radicals. Analytical Biochemistry, 165, 215–219.
25.
RubertoA, BarattaM. (2000) Antioxidant activity of selected essential oil components in two lipid model systems. Food Chemistry, 69, 167–174.
26.
MensorLL, MenezesFS, LeitaoGG, ReisAS, SantosTS, CoubeCS, LeitaoSG. (2001) Screening of Brazilian plant extracts for antioxidant activity by the use of DPPH free radical method. Phytotherapy Research, 15, 127–130.
27.
YenG, ChangY, SuS. (2003) Antioxidant activity and active compounds of rice koji fermented with Aspergillus candidus. Food Chemistry, 83, 49–54.
28.
ZainolM, Abd-HamidA, YusofS, MuseR. (2003) Antioxidative activity and total phenolic compounds of leaf, root and petiole of four accessions of Centella asiatica (L) Urban. Food Chemistry, 81, 575–581.
29.
JirovetzL, BuchbauerG, BailS, DenkovaZ, SlavchevA, StoyanovaA, SchmidtE, GeisslerM. (2009) Antimicrobial activity of essential mint and peppermint oils and some of its main compounds. Journal of Essential Oil Research, 21, 363–366.
