Chemical constituents of the essential oil from the leaves of Minthostachys mollis (Kunth) Griseb Vaught var. mollis collected in January 2008 at Tuñame, Trujillo State, Venezuela, were separated and identified by GCMS analysis. The essential oil was obtained by hydrodistillation and thirteen components (98.5% of the sample) were identified by comparison with the Wiley GCMS library data base. The two major components were pulegone (55.2%) and trans-menthone (31.5%). The essential oil showed a significant inhibitory effect against Gram-positive and Gram-negative bacteria, especially Bacillus subtilis and Salmonella typhi (4 μg/mL).
Schmidt-LebuhnAN. (2008) Monophyly and phylogenetic relationships of Minthostachys (Labiatae, Nepetoideae) examined using morphological and nr I TS data. Plant Systematics and Evolution, 270, 25–38.
2.
HeinrichM. (1992) Economic botany of American Labiatae. In: HarleyRM, ReynoldsT. (Eds.), Advances in Labiate Science. Royal Botanical Gardens, Kew, pp. 475–488.
3.
HammondGB, FernándezID, VillegasLF, ViasbergAJ. (1998) A survey of traditional medicinal plants from the Callejón de Huaylas, Department of Ancash, Perú. Journal of Ethnopharmacology, 61, 17–30.
4.
Schimidt-LebuhnAN. (2008) Ethnobotany, biochemistry and pharmacology of Minthostachys (Lamiaceae). Journal of Ethnopharmacology, 118, 343–353.
5.
CarvajalG, ThillyW. (1988) Mutagenic activity of Minthostachys mollis in AHH1 lymphoblast cells. Plant Foods for Human Nutrition, 38, 105–114.
6.
FesterGA, GargalloMA, MartinuzziEA. (1947) Algunas esencias volátiles de San Luis y Córdoba. Anales de Sociedad Científica Argentina, 144, 457–471.
7.
FesterGA. (1958) Substancias raras en esencias volátiles argentinas. Anales de la Asociación Química Argentina, 46, 15–19.
8.
FesterGA, MartinuzziEA, RetamarJA, RicciardiAIA. (1960) Estudios sobre esencias volátiles argentinas. Revista de la Facultad de Ingeniería Quimica, 29, 21–44.
9.
RojasLB, UsubillagaAN. (1995) Essential oil of Minthostachys mollis Grisebach from Venezuela. Journal of Essential Oil Research, 7, 211–213.
MalagónO, VilaR, IglesiasJ, ZaragozaT, CañigueralS. (2003) Composition of the essential oils of four medicinal plants from Ecuador. Flavour and Fragrance Journal, 18, 527–531.
12.
CalleJC, EspinosaAM, NuñezCP, BautistaE, PinzónR. (2004) Actividad insecticida del aceite esencial de Mintostachys mollis (HBK) Griseb y sus componentes. Revista Colombiana de Ciencias Químicas Farmacéuticas, 33, 137–144.
13.
DiazLK, MoromiHN. (2005) Determinación antibacteriana in vitro de Minthostachys mollis (Muña) frente a bacterias orales de importancia estomatológica. Odontología Somarquina, 8, 3–5.
14.
BravoMO, HernándezNE, TereschukML, RomeroCA, AbdalaLR. (2004) Minthostachys mollis (Griseb.) y Lepechinia meyenii (Walp.) Epling. actividad antimicrobiana de sus extractos determinaciones preliminares de sus flavonoides mayoritarios. Revista del CIZA, 5, 7–23.
15.
PrimoV, RoveraM, ZanonS, OlivaM, DemoM, DagheroJ, SabiniL. (2001) Determination of the antibacterial and antiviral activity of the essential oil from Minthostachys verticillata (Griseb.) Epling. Revista Argentina de Microbiología, 33, 113–117.
SandraP, BicchiC. (1987) Capillary gas chromatography in essential oil analysis. Huethig, Heidelberg. 435p.
18.
AdamsRP. (1995) Identification of Essential Oils Components by Gas Chromatography/Mass Spectroscopy. Allured Publishing Corporation, Carol Stream IL, USA. 456p.
19.
DaviesNW. (1990) Gas chromatographic retention indices of monoterpenes and sesquiterpenes on methyl silicone and Carbowax 20 M. phases. Journal of Chromatography A, 503, 1–24.
20.
Clinical and Laboratory Standards Institute. CLSI. (2007) Methods for dilution antimicrobial susceptibility test for bacteria that grow aerobically; Approved standard. Performance standards for antimicrobial susceptibility testing; Sixteenth informational supplement M7-A7.
