A rapid, two-solvent, HPLC-APCI-MS method was developed to identify and quantify four pentacyclic triterpenes (betulinic acid, ursolic acid, α-amyrin and β-amyrin) in extracts of the neotropical medicinal plant Souroubea sympetala. Analysis of plant organs, wood, bark, leaves, immature fruit and flowers, indicated that the phytochemical distribution and quantity of triterpenes varies across the plant, with betulinic acid and ursolic acid the major constituents in the bark, wood, fruit and flowers and the amyrins the major constituents of the leaves.
HeywoodVH. (1993) Flowering Plants of the World.B T Batsford Ltd.London.
2.
SchultesRE, RaffaufRF. (1990) The healing forest: Medicinal and toxic plants of the Northwest Amazonia. Dioscorides Press, Portland, OR
3.
KleinJ. (1978) Susto: The anthropological study of diseases of adaptation. Social Science & Medicine. Part B: Medical Anthropology, 12, 23–28
4.
WHO. (1993) The ICD-10 classification of mental and behavioural disorders: Diagnostic criteria for research. World Health Organization, Geneva
5.
APA. (1994) Diagnostic and statistical manual of mental disorders (4th ed.). American Psychiatric AssociationWashington, DC
6.
PunianiE. (2004) Novel natural product based anti-anxiety therapy and natural insecticides. PhD Thesis.Ottawa-Carleton Chemistry Institute. Ottawa, ON.
7.
AwadR, LevacD, CybulskaP, TrudeauVL, ArnasonJT. (2007) Effects of traditionally used anxiolytic botanicals on enzymes of the γ-amino butyric acid (GABA) metabolism. Canadian Journal of Physiology and Pharmacology, 85, 933–942
8.
AshtonH, YoungAH. (2003) GABA-ergic drugs: Exit stage left, enter stage right. Journal of Psychopharmacology, 17, 174–178
9.
ZwanzgerP, RupprechtR. (2005) Selective GABAergic treatment for panic? Investigations in experimental panic induction and panic disorder. Journal of Psychiatry and Neuroscience, 30, 167–175
10.
HuieCW. (2002) A review of modern sample-preparation techniques for the extraction and analysis of medicinal plants. Analytical and Bioanalytical Chemistry, 373, 23–30.
11.
SchaafO, JarvisA, van der EschSA, GiagnacovoG, OldhamN. (2000) Rapid and sensitive analysis of azadirachtin and related triterpenoids from Neem (Azadirachta indica) by high-performance liquid chromatography–atmospheric pressure chemical ionization mass spectrometry. Journal of Chromatography A, 886, 89–97
12.
ZauggJ, PotteratO, PlescherA, HonermeierB, HamburgerM. (2006) Quantitative analysis of anti-inflammatory and radical scavenging triterpenoid esters in evening primrose seeds. Journal of Agricultural and Food Chemistry, 54, 6623–6628
13.
AragãoGF, CarneiroLMV, JuniorAPF, VieiraLC, BandeiraPN, LemosTLG, VianaGSd.B. (2006) A possible mechanism for anxiolytic and antidepressant effects of alpha- and beta-amyrin from Protium heptaphyllum (Aubl.) March. Pharmacology Biochemistry and Behavior, 85, 827–834.