Two new kaempferol glycosides, kaempferol 3-O-(2″-α-L-rhamnopyranosyl)-β-L-arabinopyranoside-7-O-α-L-rhamnopyranoside and kaempferol 3-O-(2″-α-L-rhamnopyranosyl)-β-L-arabinopyranoside-7-O-α-L-rhamnopyranoside-4′-O-β-D-glucopyranoside were isolated along with four known flavonols from Matthiola longipetala (subsp. livida) (Delile) Maire growing in Egypt. Their structures were established on the basis of detailed chemical and spectral analysis. The cytotoxic activity of the alcoholic extract against Cervix (HELA) and Colon (HCT116) human cell lines showed IC50 values of 22.1 and 53.7 μg/mL, respectively. No activity was found against liver (HEPG2) carcinoma cells.
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