Dried green tea leaves, fortified with more than 190 multi-class pesticides, were analyzed by the newly modified Luke multiresidue method. This consists of acetone-acetonitrile extraction, dichloromethane partition and florisil cartridge purification, eluting with n-hexane-dichloromethane-acetonitrile (50:45:5), which was optimized in this study. The final sample, in n-hexane, was analyzed by GC-ECD and/or NPD. Of the 194 pesticides tested, 147 pesticides were recovered with more than 70% from dried green tea leaves. Eighteen pesticides were not recovered at all with this method.
AnujB. (2002) Environmental problems in tea gardens institute of advanced study in Science & Technology, Khanapana, Guwahati, Assam. Available from: http://envis.nic.in/news/newsletter_rana1.pdf. Accessed May 13, 2007.
Annex to SANCO/17/04 Final. (2002) ANNEX1 to Monitoring of pesticide residues in products of plant origin in the European Union, Norway, Iceland and Liechtenstein, 2002 Report.
5.
HajslovaJ, ZrostilikovaJ. (2003) Matrix effects in (ultra)trace analysis of pesticide residues in food and biotic matrices. Journal of Chromatography A, 1000, 181–197.
6.
LukeMA, FrobergJE, MasumotoHT. (1975) Extraction and cleanup of organochlorine, organophosphate, organonitrogen, and hydrocarbon pesticides in produce for determination by gas-liquid chromatography. Journal of the Association of Official Analytical Chemists, 58, 1020–1026.
7.
SawyerLD. (1985) The Luke et al. method for determining multipesticide residues in fruits and vegetables: collaborative study. Journal of the Association of Official Analytical Chemists, 68, 64–71.
8.
HongYS, ParkHW, ChoiH, MoonJK, KimMJ, KimJE, LeeYD, OhCH, KimJH. (2004) An improved method for multiresidue analysis of pesticides in lettuce, Chinese cabbage and green pepper by gas chromatography. Korean Journal of Environmental Agriculture, 23, 158–169.
9.
Korea Food and Drug Administration. (2002) Residual Pesticide Analysis in Foods. Separate Vol, In: Korea Food Code.Seoul, Korea, 258–263.
10.
KimMJ, JungSS, ParkJS, KimJE, LeeYD, KimJH, OhCH. (2005) Application of the pesticide multiresidue analysis method for potatoes and carrots. Korean Journal of Food Science and Technology, 37, 304–307.
11.
SchenckFJ, LehotaySJ. (1999) Does further clean-up reduce the matrix enhancement effect in gas chromatographic analysis of pesticide residues in food?Journal of Chromatography A, 868, 51–61.