Seven species of the genus Cleome were analysed for formation of exudate flavonoids. The majority of structures found were polymethoxyflavonols with 6- and/or 8-O-substitution. A novel compound, isolated from C. felina, was determined to be 5,3′,4′-triOH-3,6,7,5′-tetraOMe-flavone (1), and a further novel compound, 5,3′-diOH-3,7,8,4′,5′-pentaOMe-flavone (3), was isolated from C. viscosa. Flavones with corresponding substitution have primarily been found in C. droserifolia. Rather simple flavanones such as naringenin-7-Me have so far been found only in one accession of C. hassleriana. The flavonoid profiles of the newly studied species and accessions are discussed in relation to phytochemical, taxonomical and pharmacological data.