A method for the isolation of three compounds from the infusion of leaves of Byrsonima basiloba A. Juss. by high-speed counter-current chromatography (HSCCC) was developed. This technique led to the separation of a novel compound, quercetin 3-O-α-L-rhamnopyranosyl-(1→3)-O-[α-L-rhamnopyranosyl-(1→6)]-β-D-allopyranoside, and two known compounds quercetin-3-O-α-L-rhamnopyranosyl-(1→6)-β-D-galactopyranoside and (+)-catechin in 4 h with purities of over 92%. The structures of the compounds were determined by one- and two-dimensional NMR spectroscopy and HPLC.
SilvaSR, SilvaAP, MunhozCB, SilvaMCJr., MedeirosMB. (Eds) (2001) Guia de Plantas do Cerrado utilizadas na Chapada dos Veadeiros. WWF, Brasília.
2.
FigueiredoME, MichelinDC, SannomiyaM, da SilvaMA, dos SantosLC, de AlmeidaLFR, Souza-BritoARM, SalgadoHRN, VilegasW. (2005) Avaliação química e da atividade anti-diarréica das folhas de Byrsonima cinera DC. (Malpighiaceae). Revista Brasileira de Ciências Farmacêuticas, 41, 1–5.
3.
SannomiyaM, RodriguesCM, CoelhoRG, dos SantosLC, Hiruma-LimaCA, Souza-BritoARM, VilegasW. (2004) Application of preparative high-speed counter-current chromatography for the separation of flavonoids from the leaves of Byrsonima crassa. Niedenzu (IK). Journal of Chromatography A, 1035, 47–51.
4.
SannomiyaM, FonsecaVB, da SilvaMA, RochaLRM, dos SantosLC, Hiruma-LimaCA, Souza-BritoARM, VilegasW. (2005) Flavonoids and antiulcerogenic activity from Byrsonima crassa leaves extracts. Journal of Ethnopharmacology, 97, 1–6.
5.
SannomiyaM, MontoroP, PiacenteS, PizzaC, Souza-BritoARM, VilegasW. (2005) Application of liquid chromatography/electrospray ionization tandem mass spectrometry to the analysis of polyphenolic compounds from an infusion of Byrsonima crassa Niedenzu. Rapid Communications in Mass Spectrometry, 19, 2244–2250.
6.
SannomiyaM, MichelinDC, RodriguesCM, dos SantosLC, SalgadoHRN, Hiruma-LimaCA, Souza-BritoARM, VilegasW. (2005) Byrsonima crassa Niedenzu (IK): antimicrobial activity and chemical study. Revista de Ciências Farmacêuticas Básica e Aplicada, 26, 71–75.
7.
RastrelliL, de TommasiN, BergerI, CaceresA, SaraviaA, de SimoneF. (1997) Glycolipids from Byrsonima crassifolia.Phytochemistry, 45, 647–650.
8.
BejarE, AmarquayeA, ChecC-T, MaloneMH, FongHHS. (1995) Constituents of Byrsonima crassifolia and their spasmogenic activity. International Journal of Pharmacognosy, 33, 25–32.
WangX, WangY, GengY, LiF, ZhengC. (2004) Isolation and purification of honokiol and magnolol from cortex Magnoliae officinalis by high-speed counter-current chromatography. Journal of Chromatography A, 1036, 171–175.
11.
JiangY, LuH-T, ChenF. (2004) Preparative purification of glycyrrhizin extracted from the root of liquorice using high-speed counter-current chromatography. Journal of Chromatography A, 1033, 183–186.
12.
WeiY, ZhangT, XuG, ItoY. (2001) Application of analytical and preparative high-speed counter-current chromatography for separation of lycopene from crude extract of tomato paste. Journal of Chromatography A, 929, 169–173.
13.
LeiL, YangF, ZhangT, TuP, WuL, ItoY. (2001) Preparative isolation and purification of acteoside and 2′-acetyl acteoside from Cistanches salsa (C.A. Mey.) G. Beck by high-speed counter-current chromatography. Journal of Chromatography A, 912, 181–185.
14.
ChenL, HanY, YangF, ZhangT. (2001) High-speed counter-current chromatography separation and purification of resveratrol and piceid from Polygonum cuspidatum.Journal of Chromatography A, 907, 343–346.
15.
MarkhamKR. (1982) Techniques of Flavonoid Identification, Academic Press, London.
16.
AgrawalPK. (1989) Studies in Organic Chemistry, Vol. 39, Carbon-13 NMR of Flavonoids, Elsevier, Amsterdam.
17.
HarborneJB. (1982) The Flavonoids: Advances in Research since 1986, Chapman & Hall, New York.
