(-)-5-Acetoxy-6-(1-benzoyloxy-2-acetoxyethyl)-pyr-3-en-2-one (cleistenolide) and (-)-2,6-diacetoxy-5-hydroxy-cyclohex-3-enylidenemethyl benzoate (cleistodienol) were isolated as novel antimicrobial and cytotoxic constituents of Cleistochlamys kirkii (Annonaceae), together with (Z)-(+)-5-(2,3-dihydroxy-propylidene)-5H-furan-2-one and its acetyl and benzoyl derivatives, (-)-1,6-desoxy-β-senepoxide, pinocembrin and polycarpol. Structural determination was achieved based on spectroscopic and other physical data. The structure of cleistenolide was confirmed by single crystal X-ray crystallographic analysis.
NkunyaMHH. (2005) Unusual metabolites from some Tanzanian indigenous plant species. Pure and Applied Chemistry, 77, 1943–1955.
2.
NkunyaMHH, WeenenH, KoyiNJ, ThijsL, ZwanenburgB. (1987) Cyclohexene epoxides, (+)-pandoxide, (+)-β-senepoxide and (-)-pipoxide, from Uvaria pandensis.Phytochemistry, 26, 2563–2565.
3.
NkunyaMHH, WeenenH, BrayDH, MganiQA, MwasumbiLB. (1991) Antimalarial activity of Tanzanian plants and their active constituents: The genus Uvaria.Planta Medica, 57, 341–343.
4.
WeenenH, NkunyaMHH, MganiQA, AchenbachH, PosthumusMA, WaibelR. (1991) Lucidene, a bis(benzopyranyl) sesquiterpene from Uvaria lucida ssp. lucida. Journal of Organic Chemistry, 56, 5865–5867.
5.
FreiburghausF, JonkerSA, NkunyaMHH, MwasumbiLB, BrunR. (1997) In vitro trypanocidal activity of some rare Tanzanian medicinal plants. Acta Tropica, 67, 181–185.
6.
Sung'hwaF.MginaCA, JonkerSA, NkunyaMHH, WaibelR, AchenbachH. (1999) Ophyrepetalin and other annonaceous acetogenins from Ophrypetalum odoratum.Natural Product Letters, 13, 195–202.
7.
NkunyaMHH, MakangaraJJ, JonkerSA. (2004) Prenylindoles from Tanzanian Monodora and Isolona Species. Natural Product Research, 18, 253–258.
8.
NkunyaMHH, JonkerSA, WachiraSW, KihampaC. (2004) (±)-Schefflone: A trimeric monoterpenoid from the root bark of Uvaria scheffleri.Phytochemistry, 65, 399–404.
9.
MakangaraJJ, HenryL, JonkerSA, NkunyaMHH. (2004) The caulindoles: Dimeric prenylindoles from Isolona cauliflora.Phytochemistry, 65, 227–232.
10.
VerdcourtB. (1971) Flora of Tropical East Africa – Annonaceae, Crown Agents, London, pp 30, 124.
11.
VerzarR, PetriG. (1987) Medicinal plants in Mozambique and their popular use. Journal of Ethnopharmacology, 19, 67–80.
ShemCC, ChouSC, ChouCJ, HoLK. (1996) Total synthesis of acetylmelodorinol. Tetrahedron Asymmetry, 7, 3141–3146.
16.
ManatP, PatoomratanaT, PornchaiP, AttasitL, RentrakalV. (1999) Total synthesis of (S)-(+)-melodorinol and (S)-(+)-acetylmelodorinol. Heterocycles, 51, 795–802.
17.
KodpinidM, SadavongvivadC, ThebtaranonthC, ThebtaranonthY. (1983) Structures of β-senepoxide, tingtanoxide and their diene precursors. Constituentes of Uvaria ferruginea.Tetrahedron Letters, 24, 2019–2022.
18.
LasswellWL, HuffordCD. (1977) Cytotoxic C-benzylated flavonoids from Uvaria chamae.Journal of Organic Chemistry, 42, 1295–1296.
19.
HuffordCD, LasswellWL. (1978) Antimicrobial activities of constituents of Uvaria chamae.Lloydia, 41, 156–160.
20.
BeckerED. (1980) High resolution NMR, theory and chemical application, 2nd Ed, Academic Press, London, pp 102–104.
21.
MeyerBN, FerriginiRN, JacobsenLB, NichoilasDE, McLaughlinJL. (1982) Brine shrimp test: A convenient general bioassay for active plant constituents. Planta Medica, 45, 31–34.
22.
StahlE. (1969) Thin-layer chromatography, a laboratory handbook, Springer-Verlag, New York, p. 857.
23.
MoshiMJ, JosephCC, InnocentE, NkunyaMHH. (2004) In-vitro antibacterial and antifungal activity of extracts and compounds from Uvaria scheffleri. Pharmaceutical Biology, 42, 269–273.