Safed Musli is an important aphrodisiac herb, which forms an essential ingredient of the preparation of more than one hundred Ayurvedic formulations. It has been found to be an ideal aphrodisiac, with none of the negative side effects associated with the chemical-based products. Various plants belonging to the genera Chlorophytum and Asparagus have been in use as aphrodisiacs under the common name of ‘Safed Musli’ because of their white tuberous roots. An AFLP based experiment was carried out to differentiate the members of the ‘Safed Musli’ complex and resolve the authentication problem prevailing in the herbal drug market.
OudhiaP. (2000) Problems perceived by safed musli (Chlorophytum borivilianum) growers of Chhattisgarh (India) region: A study. Journal of Medicinal and Aromatic Plant Sciences, 23, 396–399.
2.
ShariffA, ChennaveeraiahMS. (1972) Karyomorphology of four diploid species of Chlorophytum. Nucleus, 15, 39–45.
3.
BordiaPC, JoshiA, SimlotMM. (1995) Safed moosli. In KL Chadha and Rajendra Gupta (eds.), Advances in Horticulture, Vol. II. Medicinal and aromatic plants. Malhotra Publ. House, New Delhi, India, 429–451.
4.
KhanujaSPS, ShasanyAK, DarokarMP, KumarS. (1999) Rapid isolation of PCR amplifiable DNA from the dry and fresh sample of plants producing large amounts of secondary metabolites and essential oils by modified CTAB procedure. Plant Molecular Biology Reporter, 17, 74.
5.
VosP, HogersR, BleekerM, ReijansM, van de LeeT, HornesM, FrijtersA, PotJ, PalemanJ, KuiperM, ZabeauM. (1995) AFLP a new technique for DNA fingerprinting. Nucleic Acids Research, 23, 4407–4414
6.
JaccardP. (1908) Nouvelles recherches sur la distribution florale. Bulletin de la Societe Vaudoise des Sciences Naturelles, 44, 223–270.
7.
VirkPS, Ford-LloyedBV, JacksonMT, NewburryHJ. (1995) Use of RAPD for the study of diversity within plant germplasm collections. Heredity, 74, 174–179.
8.
JainN, ShasanyAK, SundaresanV, RajkumarS, DarokarMP, BagchiGD, GuptaAK, KumarS, KhanujaSPS. (2003) Molecular diversity in Phyllanthus amarus assessed through RAPD analysis. Current Science, 85, 1454–1458.
9.
HansenM, KraftT, ChristianssonM, NilssonNO. (1999) Evaluation of AFLP in Beta. Theoretical and Applied Genetics, 98, 845–852.
10.
JonesCJ, EdwardsKJ, CastaglioneS, WinfieldMO, SalaF. (1997) Reproducibility testing of RAPD, AFLP and SSR markers in plants by a network of European laboratories. Molecular Breeding, 3, 381–390.
11.
VuylstekeM, MankR, BrugmansB, StamP, KuiperM. (2000) Further characterization of AFLP data as a tool in genetic diversity assessments among maize (Zea mays L.) inbred lines. Molecular Breeding, 6, 265–276.
12.
HillM, WitsenboerH, ZabeauM, VosP, KesseliR, MichelmoreRW. (1996) AFLP fingerprinting as a tool for studying genetic relationships in Lactuca spp. Theoretical and Applied Genetics, 93, 1202–1210.
13.
KoopmanWJM, ZevenbergenMJ, Van den BergRG. (2001) Species relationships in Lactuca S.L. (Lactuceae, Asteraceae) inferred from AFLP fingerprints. American Journal of Botany, 88, 1881–1887.
14.
Alonso-BlancoC, PeetersAJM, KoornneefM, ListerC, DeanC, van den BoschN, PotJ, KuiperMTR. (1998) Development of an AFLP based linkage map of Ler, Col and Cvi Arabidopsis thaliana ecotypes and construction of a Ler/Cvi recombinant inbred line population. Plant Journal, 14, 259–271.
15.
PolancoC, RuizML. (2002) AFLP analysis of somaclonal variation in Arabidopsis thaliana regenerated plants. Plant Science, 162, 817–824.
