Two new lanostanoid triterpenes named 3β-hydroxy-4,4,14-trimethyl-7,11,15-trioxochol-8-en-24-oic acid (1) and 3β-hydroxy-7,11,12,15,23-pentaoxo-lanost-8-en-26-oic acid (2) were isolated from the fruit bodies of Ganoderma lucidum, which is the major component of the antitumor product SunRecome®. Their cytotoxic activities were assayed in vitro against HeLa, p388, SGC-7901, BEL-7402 cell lines, and the results showed that 1 had IC50 values of 18.00 μM against p388 cell, 12.70 μM against Hela cell, 22.00 μM against BEL-7402 cell, 1.50 μM against SGC-7901 cell and 2 had IC50 values of 9.85 μM against p388 cell, 17.10 μM against Hela cell, 51.00 μM against BEL-7402 cell, 42.00 μM against SGC-7901 cell.
“Shennong Bencao Scripture” was the first monograph on traditional Chinese medicinal materials in China. It was recorded totally three hundred and sixty five Chinese medicinal materials in three classes according to their effects and toxicity, including herbs, animals, and minerals, of which, the materials with effects and without toxicity were embodied in the first class.
2.
BaoXF, WangXS, DongQ, FangJN, LiXY. (2002) Structural features of immunologically active polysaccharides from Ganoderma lucidum. Phytochemistry, 59, 175–181.
3.
KomodaY, ShimizuM, SonodaY, SatoY. (1989) Ganoderic acid and its derivatives as cholesterol synthesis inhibitors. Chemical & Pharmaceutical Bulletin, 37, 531–533.
4.
HuH, AhnNS, YangX, LeeYS, KangKS. (2002) Ganoderma lucidum extract induces cell cycle arrest and apoptosis in MCF-7 human breast cancer cell. International Journal of Cancer. Journal International du Cancer, 102, 250–253.
5.
MinBS, GaoJJ, HattoriM, LeiHK, KimYH. (2001) Anticomplement activity of terpenoids from the spores of Ganoderma lucidum. Planta Medica, 67, 811–814.
6.
GaoJJ, MinBS, AhnEM, NakamuraN, LeeHK, HattoriM. (2002) New triterpene aldehydes, lucialdehydes A-C, from Ganoderma lucidum and their cytotoxicity against murine and human tumor cells. Chemical & Pharmaceutical Bulletin, 50, 837–840.
7.
KikuchiT, MatsudaS, KadotaS, MuraiY, OgitaZ. (1985) Ganoderic acid D, E, F, and H and lucidenic acid D, E, and F, new triterpenoids from Ganoderma lucidum. Chemical & Pharmaceutical Bulletin, 33, 2624–2627.
8.
MinBS, GaoJJ, NakamuraN, HattoriM. (2000) Triterpenes from the spores of Ganoderma lucidum and their cytotoxicity against meth-A and LLC tumor cells. Chemical & Pharmaceutical Bulletin, 48, 1026–1033.
9.
EL-MekkawyS, MeselhyMR, NakamuraN, TezukaY, HattoriM, KakiuchiN, ShimotohnoK, KawahataT, OtakeT. (1998) Anti-HIV-1 and anti-HIV-1-protease substances from Ganoderma lucidum. Phytochemistry, 49, 1651–1657.
10.
MinBS, NakamuraN, HattoriM. (1998) Triterpenes from the spores of Ganoderma lucidum and their inhibitory activity against HIV-1 protease. Chemical & Pharmaceutical Bulletin, 46, 1607–1612.
11.
WuTS, ShiLS, KuoSC. (2001) Cytotoxicity of Ganoderma lucidum triterpenes. Journal of Natural Products, 64, 1121–1122.
12.
LinC, TomeW, WonS. (1991) Novel cytotoxic principles of Formosan Ganoderma lucidum. Journal of Natural Products, 54, 998–1002.
KomodaY, NakamuraH, IshiharaS, UchidaM, KohidaH, YamasakiK. (1985) Structures of new terpenoid constituents of Ganoderma lucidum (Fr.) Karst (Polyporaceae). Chemical & Pharmaceutical Bulletin, 33, 4829–4835.
15.
ChenRY, YuDQ. (1991) Studies on the triterpenoid constituents of the spores from Ganoderma lucidum Karst. Acta Pharmaceutica Sinica, 26, 267–273 (in Chinese).
16.
LiuWJ, JiangJF, XiaoD, DingJ. (2002) Down-regulation of telomerase activity via protein phosphatase 2A activation in salvicine-induced human leukemia HL-60 cell apoptosis. Biochemical Pharmacology, 64, 1677–1687.
