The cytotoxic activity of the tropane alkaloids, hederacine A (1) and hederacine B (2), previously isolated from the methanol extract of the aerial parts of Glechoma hederaceae, was assessed by the MTT cytotoxicity assay using colon cancer cell line (CaCo-2). The IC50 values for 1 and 2 were found to be 86.6 and 301.0 μM, respectively.
ShoebM, CelikS, JasparsM, KumarasamyY, MacManusSM, NaharL, Thoo-LinPK, SarkerSD. (2005) Isolation, structure elucidation and bioactivity of schischkiniin, a unique indole alkaloid from the seeds of Centaurea schischkini. Tetrahedron, 61, 9001–9006.
3.
KumarasamyY, CoxPJ, JasparsM, NaharL, SarkerSD. (2003) Isolation, structure elucidation and biological activity of two unique alkaloids, hederacine A and B, from Glechoma hederaceae, Tetrahedron, 59, 6403–6407.
4.
MossmanT. (1983) Rapid colorimetric assay for cellular growth and survival: application to proliferation and cytotoxicity assays, Journal of Immunological Methods, 65, 55–63.
5.
MeyerBN, FerrigniRN, PutnamJE, JacobsenLB, NicholsDE, MclaughlinJL. (1982) Brine shrimp: a convenient bioassay for active plant constituents, Planta Medica, 45, 31–34.
6.
SilvaGL, CuiBL, ChavezD, YouM, ChaiHB, RasoanaivoP, LynnSM, O'NeillMJ, LewisJA, BestermamJM, MonksA, FarnsworthNR, CordellGA, PezzutoJM, KinghornAD. (2001) Modulation of the multidrug-resistance phenotype by new tropane alkaloid aromatic esters from Erythroxylum pervillei, Journal of Natural Products, 64, 1514–1520.
7.
MiQW, CuiBL, LantvitD, Reyes-LimE, ChaiHY, PezzutoJM, KinghornAD, SwansonSM. (2003) Pervilleine F, a new tropane alkaloid aromatic ester that reverses multidrug resistance, Anticancer Research, 23, 3607–3615.