Sage Journals: Discover world-class research

Abstract

Although our previous study confirmed that periostin (PN) was overexpressed in the eutopic and ectopic endometrial stroma of women with endometriosis by immunohistochemitry, the role of PN in the pathophysiology of endometriosis remains unknown. Thus, we aimed to investigate the effects of PN on endometrial stromal cells (ESCs) migration, invasion, adhesion, and proliferation and to further study the mechanism under this process. Eutopic (EuSCs), ectopic (EcSCs), and normal ESCs (NSCs) were isolated and cultured. We evaluated the above-mentioned biology behaviors and the expression of PN, integrin-linked kinase 1 (ILK1), and phospho-Akt (p-Akt) in NSCs, EuSCs as well as EcSCs before and after receiving PN small-interfering RNA (siRNA). The protein and messenger RNA (mRNA) levels of PN were upregulated in EuSCs (P < .05; P = .2261 in proliferative phase and P = .3385 in secretory phase) and EcSCs (P < .001; P < .001 in proliferative phase and P < .05 in secretory phase) compared with NSCs, although there was no significant difference in PN mRNA between EuSCs and NSCs. In EcSCs, abilities of migration, invasion, and adhesion and the expressions of ILK1 and p-Akt were enhanced; and all of those were downregulated after PN siRNA interference. Thus, PN enhanced ESCs migration, invasion, and adhesion due to the ILK1/Akt signal pathway. As an agonist in the development and progression of endometriosis, PN may be a new clinical treatment target of endometriosis.

Keywords

periostin ESCs ILK1/Akt migration invasion adhesion

Get full access to this article

View all access options for this article.

References

Giudice

. Clinical practice. Endometriosis. N Engl J Med. 2010;362 (25):2389–2398.

Sampson

. Peritoneal endometriosis due to menstrual dissemination of endometrial tissue into the peritoneal cavity. Am J Obstet Gynecol. 1927;14 (2):422–469.

Lang

. Cornerstone of study on endometriosis [in Chinese]. Zhonghua Fu Chan Ke Za Zhi. 2005;40 (1):3–4.

Ayala Yanez

, Mota Gonzalez M. Endometriosis: physiopathology and investigation trends (first part) [in Spanish]. Ginecol Obstet Mex. 2007;75 (8):477–483.

Bruner-Tran

Osteen

Taylor

Sokalska

Haines

Duleba

. Resveratrol inhibits development of experimental endometriosis in vivo and reduces endometrial stromal cell invasiveness in vitro. Biol Reprod. 2011;84 (1):106–112.

Taylor

Osteen

Bruner-Tran

. Novel therapies targeting endometriosis. Reprod Sci. 2011;18 (9):814–823.

Kong

Zhang

Liu

. The complementary and alternative medicine for endometriosis: a review of utilization and mechanism. Evid Based Complement Alternat Med. 2014;2014:146383.

Rogers

PAW

D’Hooghe

Fazleabas

. Priorities for endometriosis research: recommendations from an International Consensus Workshop. Reprod Sci. 2009;16 (4):335–346.

Horiuchi

Amizuka

Takeshita

. Identification and characterization of a novel protein, periostin, with restricted expression to periosteum and periodontal ligament and increased expression by transforming growth factor beta. J Bone Miner Res. 1999;14 (7):1239–1249.

10.

Conway

Izuhara

Kudo

. The role of periostin in tissue remodeling across health and disease. Cell Mol Life Sci. 2014;71 (7):1279–1288.

11.

Jin

Norris

. Periostin mediates vascular smooth muscle cell migration through the integrins alphavbeta3 and alphavbeta5 and focal adhesion kinase (FAK) pathway. Atherosclerosis. 2010;208 (2):358–365.

12.

Liu

Huang

Qin

. Inhibition of periostin gene expression via RNA interference suppressed the proliferation, apoptosis and invasion in U2OS cells. Chin Med J (Engl). 2010;123 (24):3677–3683.

13.

Yan

Shao

. Transduction of a mesenchyme-specific gene periostin into 293T cells induces cell invasive activity through epithelial–mesenchymal transformation. J Biol Chem. 2006;281 (28):19700–19708.

14.

Morra

Moch

. Periostin expression and epithelial–mesenchymal transition in cancer: a review and an update. Virchows Arch. 2011;459 (5):465–475.

15.

Cho

Cha

Song

. Enhancement of MSC adhesion and therapeutic efficiency in ischemic heart using lentivirus delivery with periostin. Biomaterials. 2012;33 (5):1376–1385.

16.

Ontsuka

Kotobuki

Shiraishi

. Periostin, a matricellular protein, accelerates cutaneous wound repair by activating dermal fibroblasts. Exp Dermatol. 2012;21 (5):331–336.

17.

Zhang

. Prognostic significance of integrin-linked kinase1 overexpression in astrocytoma. Int J Cancer. 2010;126 (6):1436–1444.

18.

Ahmed

Oliva

Rice

Quinn

. Cell-free 59 kDa immunoreactive integrin-linked kinase: a novel marker for ovarian carcinoma. Clin Cancer Res. 2004;10 (7):2415–2420.

19.

Okamura

Yamaji

Nagashima

. Prognostic value of integrin beta1-ILK-pAkt signaling pathway in non-small cell lung cancer. Hum Pathol. 2007;38 (7):1081–1091.

20.

Yang

Zheng

. Overexpression of ILK1 in breast cancer associates with poor prognosis. Tumour Biol. 2013;34 (6):3933–3938.

21.

Hannigan

Troussard

Dedhar

. Integrin-linked kinase: a cancer therapeutic target unique among its ILK. Nat Rev Cancer. 2005;5 (1):51–63.

22.

Sakai

Docheva

. Integrin-linked kinase (ILK) is required for polarizing the epiblast, cell adhesion, and controlling actin accumulation. Genes Dev. 2003;17 (7):926–940.

23.

Edwards

Thiessen

Dragowska

Daynard

Bally

Dedhar

. Inhibition of ILK in PTEN-mutant human glioblastomas inhibits PKB/Akt activation, induces apoptosis, and delays tumor growth. Oncogene. 2005;24 (22):3596–3605.

24.

Shen

Liu

Zhang

Cui

. Characterization of periostin expression in human endometrium and endometriotic lesions. Gynecol Endocrinol. 2012;28 (10):815–818.

25.

. Autophagy inhibition promotes paclitaxel-induced apoptosis in cancer cells. Cancer Lett. 2011;307 (2):141–148.

26.

Wang

Liu

Chen

Yang

. Silencing of cofilin-1 gene attenuates biological behaviours of stromal cells derived from eutopic endometria of women with endometriosis. Hum Reprod. 2010;25 (10):2480–2488.

27.

Dimitrov

Timeva

Kyurkchiev

. Characterization of clonogenic stromal cells isolated from human endometrium. Reproduction. 2008;135 (4):551–558.

28.

McBride

Walsh

Meleady

Daly

Clynes

. Bromodeoxyuridine induces keratin protein synthesis at a posttranscriptional level in human lung tumour cell lines. Differentiation. 1999;64 (3):185–193.

29.

Berger

Stierkorb

Nickenig

. The role of the AUUUUA hexamer for the posttranscriptional regulation of the AT1 receptor mRNA stability. Biochem Biophys Res Commun. 2005;330 (3):805–812.

30.

Kim

Yoshioka

Tambe

Kushima

Okada

Inoue

. Periostin is down-regulated in high grade human bladder cancers and suppresses in vitro cell invasiveness and in vivo metastasis of cancer cells. Int J Cancer. 2005;117 (1):51–58.

31.

Kudo

. Periostin in fibrillogenesis for tissue regeneration: periostin actions inside and outside the cell. Cell Mol Life Sci. 2011;68 (19):3201–3207.

32.

Shao

Bao

Bai

. Acquired expression of periostin by human breast cancers promotes tumor angiogenesis through up-regulation of vascular endothelial growth factor receptor 2 expression. Mol Cell Biol. 2004;24 (9):3992–4003.

33.

Kii

Nishiyama

. Incorporation of tenascin-C into the extracellular matrix by periostin underlies an extracellular meshwork architecture. J Biol Chem. 2010;285 (3):2028–2039.

34.

Norris

Damon

Mironov

. Periostin regulates collagen fibrillogenesis and the biomechanical properties of connective tissues. J Cell Biochem. 2007;101 (3):695–711.

35.

Shimazaki

Nakamura

Kii

. Periostin is essential for cardiac healing after acute myocardial infarction. J Exp Med. 2008;205 (2):295–303.

36.

Zhu

Fejzo

Anderson

. Periostin promotes ovarian cancer angiogenesis and metastasis. Gynecol Oncol. 2010;119 (2):337–344.

37.

Beliard

Noel

Foidart

. Reduction of apoptosis and proliferation in endometriosis. Fertil Steril. 2004;82 (1):80–85.

38.

Borg

Markwald

. Periostin: more than just an adhesion molecule. Circ Res. 2007;101 (3):230–231.

39.

Takagi

Petre

Walz

Springer

. Global conformational rearrangements in integrin extracellular domains in outside-in and inside-out signaling. Cell. 2002;110 (5):599–511.

40.

Boulter

Van Obberghen-Schilling

. Integrin-linked kinase and its partners: a modular platform regulating cell-matrix adhesion dynamics and cytoskeletal organization. Eur J Cell Biol. 2006;85 (3–4):255–263.

41.

Yin

Pavone

Wei

Kim

. Increased activation of the PI3K/AKT pathway compromises decidualization of stromal cells from endometriosis. J Clin Endocrinol Metab. 2012;97 (1): E35–43.

42.

Samartzis

Noske

Dedes

Fink

Imesch

. ARID1A mutations and PI3K/AKT pathway alterations in endometriosis and endometriosis-associated ovarian carcinomas. Int J Mol Sci. 2013;14 (9):18824–18849.

43.

Franco-Murillo

Miranda-Rodriguez

Rendon-Huerta

. Unremitting cell proliferation in the secretory phase of eutopic endometriosis: involvement of pAkt and pGSK3beta [published online September 6, 2014.]. Reprod Sci. 2014.

44.

Castronovo

Waltregny

Kischel

. A chemical proteomics approach for the identification of accessible antigens expressed in human kidney cancer. Mol Cell Proteomics. 2006;5 (11):2083–2091.

45.

Kudo

Siriwardena

Hatano

Ogawa

Takata

. Periostin: novel diagnostic and therapeutic target for cancer. Histol Histopathol. 2007;22 (10):1167–1174.

Supplementary Material

Please find the following supplemental material available below.

For Open Access articles published under a Creative Commons License, all supplemental material carries the same license as the article it is associated with.

For non-Open Access articles published, all supplemental material carries a non-exclusive license, and permission requests for re-use of supplemental material or any part of supplemental material shall be sent directly to the copyright owner as specified in the copyright notice associated with the article.

0.00 MB

0.43 MB

Periostin Enhances Migration,Invasion,and Adhesion of Human Endometrial Stromal Cells Through Integrin-Linked Kinase 1/Akt Signaling Pathway

Abstract

Keywords

Get full access to this article

References

Supplementary Material