Matrix Metalloproteinases 2 and 9 and E-Cadherin Expression in the Endometrium During the Implantation Window of Infertile Women Before In Vitro Fertilization Treatment
Restricted accessResearch articleFirst published online April, 2015
Matrix Metalloproteinases 2 and 9 and E-Cadherin Expression in the Endometrium During the Implantation Window of Infertile Women Before In Vitro Fertilization Treatment
To evaluate the expression of endometrial matrix metalloproteinases (MMPs) 2 and 9 and E-cadherin in peri-implantation phase of infertile women who have undergone in vitro fertilization (IVF) cycles.
Methods:
This prospective study included 51 patients who underwent endometrial biopsy during the receptive phase in a menstrual cycle prior to IVF treatment. The samples were evaluated by tissue microarray for immunohistochemical study.
Results:
The expression of MMP-2, MMP-9, and E-cadherin in the endometrium prior to IVF treatment was not associated with pregnancy. There was a decrease in E-cadherin immunodetection, the higher the age of the patients, a negative relationship between E-cadherin and MMP-2, and a positive association between MMP-9 and E-cadherin.
Conclusions:
The MMP-2, MMP-9, and E-cadherin are expressed in the endometrium of infertile patients during the receptive phase of the natural menstrual cycle. However, there is no correlation between the expression of these molecules and the clinical IVF outcomes.
NikasG. Pinopodes as markers of endometrial receptivity in clinical practice. Hum Reprod. 1999;14(suppl 2):99–106.
2.
NikasGDeveliogluOHTonerJPJonesHWJr. Endometrial pinopodes indicate a shift in the window of receptivity in IVF cycles. Hum Reprod. 1999;14(3):787–792.
3.
Da MottaELSerafiniP. The treatment of infertility and its historical context. Reprod Biomed Online. 2002;5(1):65–77.
4.
BerghPANavotD. The impact of embryonic development and endometrial maturity on the timing of implantation. Fertil Steril. 1992;58(3):537–542.
5.
NikasGDrakakisPLoutradisD. Uterine pinopodes as markers of the ‘nidation window’ in cycling women receiving exogenous oestradiol and progesterone. Hum Reprod. 1995;10:1208–1213.
6.
NikasGPsychoyosA. Uterine pinopodes in peri-implantation human endometrium. Clinical relevance. Ann N Y Acad Sci. 1997;816:129–142.
7.
WilcoxAJBairdDDWeinbergCR. Time of implantation of the conceptus and loss of pregnancy. N Engl J Med. 1999;340(23):1796–1799.
8.
CavagnaMManteseJC. Biomarkers of endometrial receptivity--a review. Placenta. 2003;24(suppl B):S39–S47.
9.
BartoschCLopesJMBeiresJSousaM. Human endometrium ultrastructure during the implantation window: a new perspective of the epithelium cell types. Reprod Sci. 2011;18(6):525–539.
10.
GellersenBBrosensJ. Cyclic AMP and progesterone receptor cross-talk in human endometrium: a decidualizing affair. J Endocrinol. 2003;178(3):357–372.
11.
PopoviciRMBetzlerNKKrauseMS. Gene expression profiling of human endometrial-trophoblast interaction in a coculture model. Endocrinol. 2006;147:5662–5675.
12.
GellersenBReimannKSamalecosAAupersSBambergerAM. Invasiveness of human endometrial stromal cells is promoted by decidualization and by trophoblast-derived signals. Hum Reprod. 2010;25(4):862–873.
13.
AcostaAAElbergerLBorghiM. Endometrial dating and determination of the window of implantation in healthy fertile women. Fertil Steril. 2000;73(4):788–798.
14.
SerafiniPDa RochaAMDe Toledo OsorioCA. Protein profile of the luteal phase endometrium by tissue microarray assessment. Gynecol Endocrinol. 2009;25(9):587–592.
15.
MassovaIKotraLPMobasheryS. Structural insight into the binding motifs for the calcium ion and the non-catalytic zinc in matrix metalloproteases. Bioorg Med Chem Lett. 1998;8(7):853–858.
16.
MassovaIKotraLPFridmanRMobasheryS. Matrix metalloproteinases: structures, evolution, and diversification. FASEB J. 1998;12(12):1075–1095.
MurphyGNagaseH. Progress in matrix metalloproteinase research. Mol Aspects Med. 2008;29(5):290–308.
19.
CartwrightJEFraserRLeslieKWallaceAEJamesJL. Remodelling at the maternal-fetal interface: relevance to human pregnancy disorders. Reproduction. 2010;140(6):803–813.
20.
GrahamCHLalaPK. Mechanism of control of trophoblast invasion in situ. J Cell Physiol. 1991;148(2):228–234.
21.
AlexanderCMHansellEJBehrendtsenO. Expression and function of matrix metalloproteinases and their inhibitors at the maternal-embryonic boundary during mouse embryo implantation. Development. 1996;122(6):1723–1736.
22.
LecoKJEdwardsDRSchultzGA. Tissue inhibitor of metalloproteinases-3 is the major metalloproteinase inhibitor in the decidualizing murine uterus. Mol Reprod Develop. 1996;45(4):458–465.
23.
DasSK. Regional development of uterine decidualization: molecular signaling by Hoxa-10. Mol Reprod Develop. 2010;77(5):387–396.
24.
GumbinerBM. Cell adhesion: the molecular basis of tissue architecture and morphogenesis. Cell. 1996;84(3):345–357.
25.
HuberOBierkampCKemlerR. Cadherins and catenins in development. Curr Opin Cell Biology. 1996;8(5):685–691.
26.
LarueLAntosCButzS. A role for cadherins in tissue formation. Development. 1996;122(10):3185–3194.
27.
BarthAINathkeISNelsonWJ. Cadherins, catenins and APC protein: interplay between cytoskeletal complexes and signaling pathways. Curr Opin Cell Biol. 1997;9(5):683–690.
28.
CoutifarisCKaoLCSehdevHM. E-cadherin expression during the differentiation of human trophoblasts. Development. 1991;113(3):767–777.
29.
LiuGZhangXLinH. Effects of E-cadherin on mouse embryo implantation and expression of matrix metalloproteinase-2 and -9. Biochem Biophys Res Commun. 2006;343(3):832–828.
30.
EgebladMWerbZ. New functions for the matrix metalloproteinases in cancer progression. Nat Rev Cancer. 2002;2(3):161–174.
31.
NoeVFingletonBJacobsK. Release of an invasion promoter E-cadherin fragment by matrilysin and stromelysin-1. J Cell Sci. 2001;114(pt 1):111–118.
32.
Nawrocki-RabyBGillesCPoletteM. Upregulation of MMPs by soluble E-cadherin in human lung tumor cells. Int J Cancer. 2003;105(6):790–795.
33.
CanelMSerrelsAFrameMCBruntonVG. E-cadherin-integrin crosstalk in cancer invasion and metastasis. J Cell Sci. 2013;126(pt 2):393–401.
34.
KatayamaMHiraiSKamihagiKNakagawaKYasumotoMKatoI. Soluble E-cadherin fragments increased in circulation of cancer patients. Br J Cancer. 1994;69(3):580–585.
35.
KatayamaMHiraiSYasumotoM. Soluble fragments of e-cadherin cell-adhesion molecule increase in urinary-excretion of cancer-patients, potentially indicating its shedding from epithelial tumor-cells. Int J Oncol. 1994;5(5):1049–1057.
36.
DongWZhangHLiJ. Estrogen induces metastatic potential of papillary thyroid cancer cells through estrogen receptor alpha and beta. Int J Endocrinol. 2013;2013:941568.
37.
NoyesRWHertigATRockJ. Dating the endometrial biopsy. Am J Obstet Gynecol. 1975;122(37):262–263.
38.
RobboySJMutterGLPratJBentleyRCRussellPAndersonMC. Endometrium: normal. In: Limited CLaioE, ed. Pathology of the female reproductive tract. Churchill Livingstone, Elsevier limited. 2009.
39.
PiresARAndreiuolo FdaMde SouzaSR. TMA for all: a new method for the construction of tissue microarrays without recipient paraffin block using custom-built needles. Diagn Pathol. 2006;1:14.
40.
ZhaoYBrezinaPHsuCCGarciaJBrinsdenPRWallachE. In vitro fertilization: four decades of reflections and promises. Biochim Biophys Acta. 2011;1810(9):843–852.
41.
DiedrichKFauserBCDevroeyPGriesingerGEvian Annual Reproduction Workshop G. The role of the endometrium and embryo in human implantation. Hum Reprod Update. 2007;13(4):365–377.
42.
PotdarNGelbayaTNardoLG. Endometrial injury to overcome recurrent embryo implantation failure: a systematic review and meta-analysis. Reprod Biomed Online. 2012;25(6):561–571.
43.
BarashADekelNFieldustSSegalISchechtmanEGranotI. Local injury to the endometrium doubles the incidence of successful pregnancies in patients undergoing in vitro fertilization. Fertil Steril. 2003;79(6):1317–1322.
44.
KarimzadeMAOskouianHAhmadiSOskouianL. Local injury to the endometrium on the day of oocyte retrieval has a negative impact on implantation in assisted reproductive cycles: a randomized controlled trial. Arch Gynecol Obstet. 2010;281(3):499–503.
45.
MatsuzakiSDarchaCMaleyssonECanisMMageG. Impaired down-regulation of E-cadherin and beta-catenin protein expression in endometrial epithelial cells in the mid-secretory endometrium of infertile patients with endometriosis. J Clin Endocrinol Metab. 2010;95(7):3437–3445.
46.
PonceletCCornelisFTepperM. Expression of E- and N-cadherin and CD44 in endometrium and hydrosalpinges from infertile women. Fertil Steril. 2010;94(7):2909–2912.
47.
MarinakisGNikolaouD. What is the role of assisted reproduction technology in the management of age-related infertility?Hum Fertil. 2011;14(1):8–15.
48.
ZhangZZhangLJiaL. AP-2alpha suppresses invasion in BeWo cells by repression of matrix metalloproteinase-2 and -9 and up-regulation of E-cadherin. Mol Cell Biochem. 2013;381(1-2):31–39.
49.
NamJMAhmedKMCostesS. beta1-integrin via NF-kappaB signaling is essential for acquisition of invasiveness in a model of radiation treated in situ breast cancer. Breast Cancer Res. 2013;15(4):R60.
