To evaluate the effect of resveratrol on an experimentally induced endometriosis rat model.
Study design:
After endometriotic implants were surgically formed, rats were randomly divided into 2 groups as control group (saline treated, n = 6) and resveratrol group (10 mg/kg/d, n = 6). The inflammatory markers and histopathological changes were assessed at the end of the treatment period.
Results
Our results showed (1) significant reduction in the implant size (P < .0005); (2) significantly decreased levels of vascular endothelial growth factor (VEGF) in the peritoneal fluid and plasma (P < .005); and monocyte chemotactic protein 1 (MCP-1) in the peritoneal fluid (P < .05), (3) highly significant suppression of VEGF expression in the endometriotic tissue (P < .0005); and (4) considerable histological changes in the endometriotic foci following resveratrol treatment.
Conclusion:
Resveratrol appears to be effective on the development of endometriosis through its antiangiogenic and anti-inflammatory properties. Future studies with different doses of resveratrol might provide more comprehensive results regarding the treatment of endometriosis.
YuWFuYCWangW. Cellular and molecular effects of resveratrol in health and disease. J Cell Biochem. 2012;113(3):752–759.
10.
MarkusMAMorrisBJ. Resveratrol in prevention and treatment of common clinical conditions of aging. Clin Interv Aging. 2008;3(2):331–339.
11.
ZhongMChengGFWangWJGuoYZhuXYZhangJT.Inhibitory effect of resveratrol on interleukin 6 release by stimulated peritoneal macrophages of mice. Phytomedicine. 1999;6(2):79–84.
12.
MiuraTMuraokaSIkedaNWatanabeMFujimotoY. Antioxidative and prooxidative action of stilbene derivatives. Pharmacol Toxicol. 2000;86(5):203–208.
13.
ChenCJYuWFuYCWangXLiJLWangW. Resveratrol protects cardiomyocytes from hypoxia-induced apoptosis through the SIRT1-FoxO1 pathway. Biochem Biophys Res Commun. 2009;378(3):389–393.
14.
KangOHJangHJChaeHS. Anti-inflammatory mechanisms of resveratrol in activated HMC-1 cells: pivotal roles of NF-kappaB and MAPK. Pharmacol Res. 2009;59(5):330–337.
15.
CsakiCMobasheriAShakibaeiM. Synergistic chondroprotective effects of curcumin and resveratrol in human articular chondrocytes: inhibition of IL-1beta-induced NF-kappaB-mediated inflammation and apoptosis. Arthritis Res Ther. 2009;11(6):R165.
16.
VernonMWWilsonEA. Studies on the surgical induction of endometriosis in the rat. Fertil Steril. 1985;44(5):684–694.
17.
GüneyMOralBKarahanNMunganT. Regression of endometrial explants in a rat model of endometriosis treated with melatonin. Fertil Steril. 2008;89(4):934–942.
18.
BusquetsSAmetllerEFusterG. Resveratrol, a natural diphenol, reduces metastatic growth in an experimental cancer model. Cancer Lett. 2007;245(1-2):144–148.
19.
KeenanJAWilliams-BoycePKMasseyPJChenTTCaudleMRBukovskyA. Regression of endometrial explants in a rat model of endometriosis treated with the immune modulators loxoribine and levamisole. Fertil Steril. 1999;72(1):135–141.
20.
Harrison-WoolrychMLSharkeyAMCharnock-JonesDSSmithSK. Localization and quantification of vascular endothelial growth factor messenger ribonucleic acid in human myometrium and leiomyomata. J Clin Endocrinol Metab. 1995;80(6):1853–1858.
21.
Bruner-TranKLOsteenKGTaylorHSSokalskaAHainesKDulebaAJ. Resveratrol inhibits development of experimental endometriosis in vivo and reduces endometrial stromal cell invasiveness in vitro. Biol Reprod. 2011;84(1):106–112.
22.
KunduJKShinYKKimSHSurhYJ. Resveratrol inhibits phorbol ester-induced expression of COX-2 and activation of NF-kappaB in mouse skin by blocking kappaB kinase activity. Carcinogenesis. 2006;27(7):1465–1474.
23.
KumarASharmaSS. NF-kappaB inhibitory action of resveratrol: a probable mechanism of neuroprotection in experimental diabetic neuropathy. Biochem Biophys Res Commun. 2010;394(2):360–365.
MahnkeJLDawoodMYHuangJC. Vascular endothelial growth factor and interleukin 6 in peritoneal fluid of women with endometriosis. Fertil Steril. 2000;73(1):166–170.
26.
KupkerWSchultze-MosgauADiedrichK. Paracrine changes in the peritoneal environment of women with endometriosis. Hum Reprod Update. 2000;4(5):719–723.
27.
D’AntonioMMartelliFPeanoSPapoianRBorrelliF. Ability of recombinant human TNF binding protein-1 (r-hTBP-1) to inhibit the development of experimentally-induced endometriosis in rats. J Reprod Immunol. 2000;48(2):81–98.
28.
IwabeTHaradaTTsudoT. Tumor necrosis factor-alpha promotes proliferation of endometriotic stromal cells by inducing interleukin 8 gene and protein expression. J Clin Endocrinol Metab. 2000;85(2):824–829.
29.
KangLHengWYuanABaolinLFangH. Resveratrol modulates adipokine expression and improves insulin sensitivity in adipocytes: relative to inhibition of inflammatory responses. Biochimie. 2010;92(7):789–796.
30.
GazvaniRTempletonA. Peritoneal environment, cytokines and angiogenesis in the pathophysiology of endometriosis. Reproduction. 2002;123(2):217–226.
31.
AriciAOralEAttarETazukeSIOliveDL. Monocyte chemotactic protein-1 concentration in peritoneal fluid in patients with endometriosis and its modulation in human mesothelial cells. Fertil Steril. 1997;67(6):1065–1072.
32.
UlukusMUlukusECTavmergen GokerENTavmergenEZhengWAriciA. Expression of interleukin-8 and monocyte chemotactic protein 1 in women with endometriosis. Fertil Steril. 2009;91(3):687–693.
